The effect of inoculum on the performance of sulfate-reducing columns treating heavy metal contaminated water

Pruden, Amy; Messner, N.; Pereyra, Luciana P.; Hanson, Richard E.; Hiibel, Sage R.; Reardon, Kenneth F.

doi:10.1016/j.watres.2006.11.025

Cited by 81 publications

(64 citation statements)

References 34 publications

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“…The master mix composition and temperature conditions for PCR were described by Pruden et al (2007) and Watanabe et al (2001), respectively. The PCR products were quantified using Low DNA Mass TM Ladder (Invitrogen, Carlsbad, CA) in a 1.2% agarose gel.…”

Section: Pcr Amplificationmentioning

confidence: 99%

“…The controlling influence of microbial inoculum on the treatment of MD (Chang et al, 2000;Jong and Parry, 2003;Pruden et al, 2007) as well as on the bioremediation of other contaminants such as nitrate (Moreno et al, 2005), hydrocarbons (Juteau et al, 2003), chlorinated aliphatics (Hourbron et al, 2000), and polychlorinated biphenyls (Fava and Bertin, 1999) has been demonstrated. However, no studies have systematically examined the relationship between the microbial composition of several types of inocula and their ability to remediate MD.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of microbial community composition and activity in sulfate‐reducing batch systems remediating mine drainage

Pereyra¹,

Hiibel²,

Pruden³

et al. 2008

Biotech & Bioengineering

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Five microbial inocula were evaluated in batch tests for the ability to remediate mine drainage (MD). Dairy manure (DM), anaerobic digester sludge, substrate from the Luttrell (LUTR) and Peerless Jenny King (PJK) sulfate-reducing permeable reactive zones (SR-PRZs) and material from an MD-treatment column that had been inoculated with material from a previous MD-treatment column were compared in terms of sulfate and metal removal and pH neutralization. The microbial communities were characterized at 0, 2, 4, 9, and 14 weeks using denaturing gradient gel electrophoresis and quantitative polymerase chain reaction to quantify all bacteria and the sulfate-reducing bacteria of the genus Desulfovibrio. The cultures inoculated with the LUTR, PJK, and DM materials demonstrated significantly higher rates of sulfate and metal removal, and contained all the microorganisms associated with the desired functions of SR-PRZs (i.e., polysaccharide degradation, fermentation, and sulfate reduction) as well as a relatively high proportion of Desulfovibrio spp. These results demonstrate that inoculum influences performance and also provide insights into key aspects of inoculum composition that impact performance. This is the first systematic biomolecular examination of the relationship between microbial community composition and MD remediation capabilities.

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Section: Pcr Amplificationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of microbial community composition and activity in sulfate‐reducing batch systems remediating mine drainage

Pereyra¹,

Hiibel²,

Pruden³

et al. 2008

Biotech & Bioengineering

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“…Previous studies used molecular methods to identify sulphate-reducing and organic matter degrading taxonomic groups in BCRs [22,43]. These previous methods were insufficient to capture the diversity of microbial species expected in anaerobic, organic-rich biomes.…”

Section: Microbial Communities Supported By the Hay-and Wood-rich Bcrmentioning

confidence: 99%

“…Some previous attempts have been made to identify key functional groups and their activity [21]. The types of microbes introduced into BCRs at start-up influence their operation [22], with inocula previously acclimated to high-sulphate environments performing better than sources obtained from non-sulphate environments, such as livestock manure and anaerobic digesters [23]. Since the properties of organic materials change with age of the BCR, this might be expected to impact the types of microbes present.…”

Section: Introductionmentioning

confidence: 99%

Decline in Performance of Biochemical Reactors for Sulphate Removal from Mine-Influenced Water is Accompanied by Changes in Organic Matter Characteristics and Microbial Population Composition

Mirjafari

Baldwin

2016

Water

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Successful long-term bioremediation of mining-influenced water using complex organic matter and naturally-occurring microorganisms in sub-surface flow constructed wetlands requires a balance between easily and more slowly degrading material. This can be achieved by combining different types of organic materials. To provide guidance on what mixture combinations to use, information is needed on how the ratio of labile to recalcitrant components affects the degradation rate and the types of microbial populations supported. To investigate this, different ratios of wood and hay were used in up-flow column bioreactors treating selenium-and sulphate-containing synthetic mine-influenced water. The degradation rates of crude fibre components appeared to be similar regardless of the relative amounts of wood and hay. However, the nature of the degradation products might have differed in that those produced in the hay-rich bioreactors were more biodegradable and supported high sulphate-reduction rates. Microorganisms in the sulphate-reducing and cellulose-degrading inocula persisted in the bioreactors indicating that bio-augmentation was effective. There was a shift in microbial community composition over time suggesting that different microbial groups were involved in decomposition of more recalcitrant material. When dissolved organic carbon (DOC) was over-supplied, the relative abundance of sulphate-reducers was low even through high sulphate-reduction rates were achieved. As DOC diminished, sulphate-reducers become more prevalent and their relative abundance correlated with sulphate concentrations rather than sulphate-reduction rate.

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“…Particularly notable is the high degree of bacterial diversity that has been observed in these systems using 16S rRNA gene-based methods Pereyra et al 2008;Pruden et al 2007). SRB have been discovered to represent only a small fraction of the microbial community, which makes their detection implementing 16S rRNA gene-targeted fingerprinting methods, such as denaturing gradient gel electrophoresis (DGGE), extremely difficult.…”

Section: Introductionmentioning

confidence: 99%

Response and Recovery of Sulfate-Reducing Biochemical Reactors From Aerobic Stress Events

Perrault¹,

Pereyra²,

Hiibel³

et al. 2009

JASMR

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Abstract. Microbially-mediated treatment of mining-influenced water (MIW) through the implementation of sulfate-reducing biochemical reactors (BCRs) is an attractive option for passive, in situ remediation with low operating costs and reduced maintenance requirements. However, BCRs can be unpredictable in terms of how they recover from environmental stresses such as oxygen exposure. Previous studies have demonstrated that the inoculum can impact performance positively, suggesting that engineered control of the microbial community structure could improve the resilience of BCRs to stress. The purpose of this study was to determine the effect of bioaugmentation and biostimulation on performance and recovery from oxygen stress. Twelve columns (six conditions in duplicate) were packed with a complex organic substrate (wood chips, etc.), inoculated with dairy manure, and fed simulated acid mine drainage containing Fe, Cd, and Zn at pH 5.5. The conditions tested were: 1.) bioaugmentation with cellulose degraders (CD); 2.) bioaugmentation with sulfate reducers (SRB); 3.) biostimulation with ethanol (EtOH); 4.) biostimulation with carboxymethyl cellulose (CMC); 5.) dairy manure only control (DM); 6.) un-inoculated control (CR). Once the columns reached steady state anaerobic performance, they were exposed to oxygen, allowed to reach steady state again, and then oxygen-exposed for a second, longer time. The results indicate that all columns performed well in terms of pH neutralization and removal of sulfate and heavy metals. However, the cellulose and ethanol biostimulated columns appeared to be the most resilient to oxygen exposure and performed best by the end of the experiment (171 days), while the bioaugmented columns performed similarly to the controls. The microbial communities were evaluated at each steady state using a suite of biomolecular tools, including active community profiling (ACP) and quantitative realtime PCR (Q-PCR) targeting key functional groups of sulfate-reducers, cellulosedegraders, fermenters, and methanogens. It was found that the active microbial community structure was distinct among the six column conditions and that bioaugmentation significantly impacted the kinds and diversity of bacteria present following stress events. Functional gene analysis supported these observations, but provided finer resolution regarding the response of each functional group to stress. Additional

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The effect of inoculum on the performance of sulfate-reducing columns treating heavy metal contaminated water

Cited by 81 publications

References 34 publications

Comparison of microbial community composition and activity in sulfate‐reducing batch systems remediating mine drainage

Comparison of microbial community composition and activity in sulfate‐reducing batch systems remediating mine drainage

Decline in Performance of Biochemical Reactors for Sulphate Removal from Mine-Influenced Water is Accompanied by Changes in Organic Matter Characteristics and Microbial Population Composition

Response and Recovery of Sulfate-Reducing Biochemical Reactors From Aerobic Stress Events

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