The Effect of Inter-Plantal Competition on in Vitro Seed Germination and Seedling Growth in Flax (Linum Usitatissimum L.)

Beyaz, Ramazan; Yıldız, Mustafa

doi:10.18036/aubtdc.427128

Cited by 3 publications

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“…The success of using in vitro culture to obtain callus cultures and indirect organogenesis of common fl ax depends, fi rst of all, on several components of the cultivation conditions: the type of nutrient medium [3,7]; the presence of a carbohydrate source -sucrose, glucose, maltose, lactose or their combination [8,9]; the phytohormonal composition of the growth medium, which induces intensive callus formation and organogenesis [10,11]; the genetic characteristics of the breeding material, the type and size of explants [9,12] and the characteristics of their preliminary preparation for inoculation [11,13], etc.…”

Section: Introductionmentioning

confidence: 99%

Influence of explant type and variety of Linum usitatissimum L. convar. elongatum on the intensity of callus formation and organogenesis in vitro

Mishchenko,

Kryvosheeva,

Lavrynenko

et al. 2023

PVSandP

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Purpose. To determine the dependence of the intensity of callus formation and organogenesis of Linum usitatissimum L. convar. elongatum in vitro on explant type and variety in order to optimize the cultivation protocol. Methods. For induction of callus formation and organogenesis, hypocotyls, cotyledons, leaves, immature embryos and anthers of flax varieties ‘Hlinum’, ‘Esman’, ‘Hladiator’, ‘Hlobus’ and ‘Charivnyi’ grown on Murashige and Skoog medium were treated with 0.05 mg/l 1naphthylacetic acid and 1.0 mg/l 6benzylaminopurine at a photoperiod of 16 h, light intensity 2500 lux, relative humidity 60–80% and air temperature 22–24 °C. Empirical data were interpreted using arithmetic mean, error of the sample mean, coefficient of variation, least significant difference and rank order. Results. The intensity of callus formation and organogenesis in the analysed varieties depended on the object of study, i.e. the genotype of the variety and the type of explant. The frequency of callus formation ranged from 9.4 (anthers of variety ‘Esman’) to 99.4% (leaf explants of variety ‘Hlinum’), the weight of callus – from 0.18 (anthers of variety ‘Esman’) to 3.18 g (anthers of variety ‘Hlobus’), the frequency of organogenesis – from 7.4 (anthers of variety ‘Esman’) to 97.3% (hypocotyls of variety ‘Hlinum’), number of shoots – from 0.6 (anthers of variety ‘Hladiator’ and immature embryos of variety ‘Hlobus’) to 4.0 (hypocotyls of variety ‘Hlinum’), height of shoots – from 0.34 (anthers of variety ‘Esman’) to 1.63 cm (anthers of variety ‘Hlobus’). Conclusions. Plants of all the varieties studied are capable of effective callus formation and organogenesis in vitro in the presence of phytohormones of exogenous origin. Certain types of explants (hypocotyls, cotyledons, leaves) respond stably to exogenous growth regulators that induce callus formation, whereas others, such as anthers, have a specific response that is largely determined by cultivar characteristics. To obtain diploid somaclones, it is optimal to use hypocotyls of varieties ‘Hlinum’ and ‘Charivnyi’, to obtain haploid regenerants – immature embryos and anthers of varieties ‘Hlobus’ and ‘Hladiator’, which ensures the highest reproduction rate of cultural plant objects.

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Section: Introductionmentioning

confidence: 99%

Influence of explant type and variety of Linum usitatissimum L. convar. elongatum on the intensity of callus formation and organogenesis in vitro

Mishchenko,

Kryvosheeva,

Lavrynenko

et al. 2023

PVSandP

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“…При цьому для досягнення поставленої мети використовують досить різні поєднання фітогормонів та вуглеводів у живильному середовищі [5][6][7][8][9][10][11][12]. Ефективність органогенезу залежить ще й від попередньої підготовки експлантів (гіпокотильних сегментів) та конкуренції між ними [13,14].…”

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Effect of 1-naphthylacetic and indol-3-acetics acid on the intensity of callusogenesis and organogenesis of Linum usitatissimum L. in vitro

Mishchenko¹

2021

Fakt. eksp. evol. org.

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Aim. Investigate the effect of auxins of exogenous origin in nutrient medium in vitro on the germination and organogenesis intensity in Linum usitatissimum L. convar. elongatum (‘Hlinum’ variety) at the constant concentration of 6-benzylaminopurine (BAP). Methods. Hypocotyl segments were cultured on Murashige and Skoog nutrient medium supplemented with sucrose (30 g/l) and phytohormones at various concentrations. Other conditions: photoperiod 16 hours, relative humidity 60–80%, air temperature 22–24°C. Results. Common flax has a great capacity to form callus and shoots under the effect of the following factors: 1) only auxins, 2) only cytokines, 3) combinations of auxins and cytokines. Somatic embryogenesis is also possible on a nonhormonal nutrient medium. Conclusions. For somatic embryogenesis in vitro, the optimal concentrations of BAP can be expressed as 1.0 ≤ BAP ≤ 1.75, the optimal concentrations of BAP for the medium supplemented with 1-naphthylacetic (NAA, 0.05 mg/l) 0.5 ≤ BAP ≤ 2.0, the optimal concentration of NAA for the medium supplemented with BAP (1.0 mg/l) 0.025 ≤ NAA ≤ 0.150, and the optimal concentrations of indol-3-acetics acid (IAA) for the medium supplemented with BAP (1.0 mg/l) 0.05 ≤ IAA ≤ 0.50. Keywords: Linum usitatissimum L., in vitro, phytohormones, callus, organogenesis.

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Callus formation, organogenesis and microclonal reproduction in different species of the genus Linum L. in vitro

Mishchenko¹,

Кривошеєва²

2019

Plant Varieties Studying and Protection

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Purpose. To reveal the frequency and intensity of callus formation and organogenesis, the effectiveness of microclonal reproduction of various species of the genus Linum L. (Linaceae) in vitro. Methods. For in vitro induction of callus formation and organogenesis, hypocotyl segments of species Linum usitatissimum L. convar. elongatum and convar. usitatissimum, L. tenue Desf., L. bienne Mill., L. corymbulosum Pchb., L. nervosum Waldst. & Kit., L. flavum L., L. campanulatum L., L. perenne L., L. austriacum L., L. grandiflorum Desf., L. strictum L. were cultivated on Murashige and Skoog medium supplemented with 0.05 mg/l 1-naphthylacetic acid and 1.0 mg/l 6-benzyl aminopurine at 22-24 °C, relative humidity of 60-80%, with 16 hours photoperiod (2500 flux). For microclonal reproduction Murashige and Skoog, White, Gamborg and Eveleigh media and their modifications were used. The measurement results were interpreted by the arithmetic mean, standard error for the sample mean, the least significant difference and ranked. Results. Different species of the genus Linum to a large extend are capable of forming callus and regenerating shoots under the specified cultivation conditions. The frequency of callus formation for the studied samples on the 35th day of cultivation varied within 81.25-100%, the mass of callus from one explant -0.21-1.64 g, the frequency of organogenesis -12.50-100%, the number of shoots -1.8-7.6 pcs. and the height of the shoots was 0.82-2.12 cm. The following species: L. usitatissimum convar. elongatum, L. tenue, L. bienne and L. strictum were distinguished by a high intensity of callus formation. Intensive organogenesis was pecular to L. tenue, L. bienne, L. flavum, L. austriacum and L. grandiflorum. The efficiency of somaclone obtaining was quite low in L. nervosum and L. campanulatum. In total, for the microclonal reproduction of species of the genus Linum Murashige and Skoog, Gamborg and Eveleigh media supplemented with 12.5 g/l glucose were optimal. At the final stages of microclonal propagation, before transferring microclones in vivo, it is advisable to use White medium, which contributes to a high frequency of rhizogenesis. Varieties of L. usitatissimum convar. elongatum and convar. usitatissimum had different responses to in vitro culture. Conclusions. The frequency and intensity of callus formation and organogenesis, the effectiveness of microclonal reproduction depended on the genotype of a particular species; therefore it is advisable to select the composition of the nutrient medium and growth regulators for each of them. Some species of the genus Linum have not yet been studied in vitro, so the obtained results allow expanding the scope of their use in practice, in particular in breeding as a new source material with somaclonal variation, interspecific crosses, and ornamental floriculture.

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The Effect of Inter-Plantal Competition on in Vitro Seed Germination and Seedling Growth in Flax (Linum Usitatissimum L.)

Cited by 3 publications

References 19 publications

Influence of explant type and variety of Linum usitatissimum L. convar. elongatum on the intensity of callus formation and organogenesis in vitro

Influence of explant type and variety of Linum usitatissimum L. convar. elongatum on the intensity of callus formation and organogenesis in vitro

Effect of 1-naphthylacetic and indol-3-acetics acid on the intensity of callusogenesis and organogenesis of Linum usitatissimum L. in vitro

Callus formation, organogenesis and microclonal reproduction in different species of the genus Linum L. in vitro

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