The Effect of Intracellular Oxygen Concentration on Ventricular Fibrillation in Perfused Rat Heart

Makiguchi, Mitsuyuki; Kawaguchi, Hideaki; Yasuda, Hisakazu; Tamura, Mamoru

doi:10.1007/978-1-4684-7433-6_35

Cited by 1 publication

(1 citation statement)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although ventricular fibrillation induced by electrical stimulation differs from spontaneous fibrillation, determination of the VFT appears to be valuable in the experimental evaluation of vulnerability to fibrillation. This is supported by our experiments showing that early acute hypoxia and catecholamine levels that tend to produce arrhythmias cause the VFT to fall [18]. The present study demonstrated that high molar ratios of albumin-bound FFA were arrhythmogenic in themselves, a result that agrees with the findings of Willebrands et al [9].…”

Section: Discussionsupporting

confidence: 93%

Effect of palmitic acid and fatty acid binding protein on ventricular fibrillation threshold in the perfused rat heart

Makiguchi

Kawaguchi

Tamura

et al. 1991

Cardiovasc Drug Ther

View full text Add to dashboard Cite

The effects of increased free fatty acid (FFA) levels on ventricular arrhythmias remain controversial. Using ventricular fibrillation threshold (VFT), we examined the relationship between FFA levels and ventricular arrhythmias. Isolated rat hearts were perfused with palmitate bound to either albumin or fatty acid binding protein (FABP) by Langendorf's method. The VFT was determined by electrical stimulation. Perfusion with 0.12 mM albumin alone, 0.12 mM palmitate bound to 0.12 mM albumin, and 0.36 mM palmitate bound to 0.12 mM albumin did not lower the VFT significantly. However, 0.60 mM palmitate bound to 0.12 mM albumin lowered VFT from 2.19 +/- 0.20 mA to 1.56 +/- 0.13 mA. The perfusion of 0.36 mM palmitate bound to 0.12 mM FABP lowered the VFT from 2.05 +/- to 0.19 mA to 1.47 +/- 0.23 mA, but 0.12 mM FABP alone did not affect the VFT. Perfusion with 0.36 mM palmitate bound to 0.12 mM FABP caused the VFT to fall more than perfusion with 0.36 mM palmitate bound to 0.12 mM albumin. Then the effects of verapamil perfusion or a low concentration of perfusate Ca2+ on VFT were examined. VFT was determined by electrical stimulation. Palmitate (0.6 mM) bound to 0.12 mM albumin lowered VFT. Verapamil 10(-7) M perfusion and a low concentration of Ca2+ (Ca2+ 1.67 mM) suppressed the FFA-induced fall of VFT. These results suggested that the arrhythmogenic action of FFA was related to Ca2+ overload in myocardial cells.

show abstract

Section: Discussionsupporting

confidence: 93%