The Effect of Intraportal Six-hydroxy-dopamine on Hemorrhage after Standardized Liver Trauma in Rats

Lindfeldt, J.; Zoucas, Evita; Ekelund, Mats; Cm, Kullendorff; Holmin, T.

doi:10.1097/00005373-198703000-00016

Cited by 6 publications

(5 citation statements)

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“…Blood loss in the liver injury model with no treatment was 357.57 ± 267.31 µL and lasted the entire experimental period. These findings are similar to those in the literature when 2.5% to 4% of the liver was resected and bleeding lasted between 5–9 minutes with 1.0 to 3.4 mL of blood lost (Alwmark et al, 1986; Bengmark et al, 1980; Bengmark et al, 1981; Holcomb et al, 2000; Kullendorff et al, 1984; Lindfeldt et al, 1987; Tanaka et al, 1985; Zoucas et al, 1982; Zoucas et al, 1984a; Zoucas et al, 1984b;). Additionally, Holcomb et al (2000) demonstrated a reduction in blood loss by 52% using fibrin glue in a model of median hepatic lobe injury in rats.…”

Section: Discussionsupporting

confidence: 89%

“…Evaluations of existing techniques for determining blood loss were imprecise and highly variable. Historically, blood loss determinations in models of liver, spleen or kidney wound were performed using a preweighed aluminum cup (Bengmark et al, 1980; Kullendorff et al, 1984; Kullendorff and Zoucas, 1985; Lindblom et al, 1990; Lindfeldt et al, 1987; Vagianos et al, 1987; Zoucas et al, 1984a; Zoucas et al, 1984b;) or a stack of gauze pads (Holcomb et al, 2000; Tuthill et al, 2001) and determining final blood loss by weight. A more extensive search of the literature provided a protocol for hematin determination (Masci et al, 1988) and coupled with the use of Whatman filter paper “chads” this provided the desired accuracy.…”

Section: Discussionmentioning

confidence: 99%

“…Resection varied from approximately 2.5–65% of the liver (Alwmark et al, 1986; Bengmark et al, 1980; Kullendorff et al, 1984; Kullendorff and Zoucas, 1985; Lindfeldt et al, 1987; Vagianos et al, 1987; Zoucas et al, 1982), 6–10% of kidney (Jackson et al, 1998; Kullendorff and Zoucas, 1985, Raccuia et al, 1992) or 1% of the caudal pole of the spleen (Lindblom et al, 1990). Most papers stated that resection followed a standardized technique, although only one article employed the use of a template to resect a standardized 10% of the kidney, thereby producing an actively bleeding area of 1×5 cm (Raccuia et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

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Hemostatic properties of a venomic protein in rat organ trauma

Warner¹,

McClintock²,

Barron³

et al. 2009

Experimental and Molecular Pathology

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Previous in vitro work characterized the protease Q8009 isolated from the venom of the Australian brown snake Pseudonaja textilis textilis with Factor Xa-like activity and hemostatic properties. The purpose of the work described here characterizes the in vivo hemostatic properties in a rat model of parenchymatous organ injury. The key parameters of activity included reduction in time-tohemostasis and total volume of blood loss in spleen, liver and kidney wound models in rats. The surgical protocols involved exposure of the organs via a midline abdominal laparotomy. Using a clean metal template with 6, 6.5 9 mm holes for spleen, liver and kidney, respectively, a predetermined volume of the organ was gently extruded through the template hole and excised with a razor blade. About 50 to 75 µL of collagen matrix with the different test solutions was applied to the wounds. Blood was collected and at the end of the procedure animals were humanely sacrificed with an anesthetic overdose. Determination of blood was performed using the hematin assay using a standard curve. Blood loss per minute and total blood loss were calculated. Results from the studies demonstrated that the application of Q8009 and collagen matrix to surgical wounds significantly reduced the total amount of blood loss and the time-to-hemostasis. In the spleen wound model, Q8009 at 100, 250 and 1000 µg/ml) significantly reduced (p<0.001) the total volume of blood lost relative to thrombin and reduced the time-to-hemostasis by 25-50%, as compared to 7% by thrombin. In the liver wound model, Q8009 at 250 and 1000 µg/ml significantly reduced (p<0.001) the total volume of blood lost relative to thrombin and reduced the time-to-hemostasis from 10.5 minutes by thrombin to 5.6 minutes with Q8009. In the kidney wound model, Q8009 at 250 µg/ml significantly reduced (p<0.05) the total volume of blood lost and reduced the time-to-hemostasis by 25% when compared to thrombin. The hemostasis levels were consistent with previous findings in skin wound rat models where Q8009 consistently reduced the total volume of blood lost and shortened time-to-hemostasis. Application of Q8009 plus collagen matrix significantly reduced the volume of total blood loss and time-to-hemostasis in rat surgical organ wound models induced bleeding, as compared to a commercially available hemostat device. The protein Q8009 has greater capacity to reduce blood

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Hemostatic properties of a venomic protein in rat organ trauma

Warner¹,

McClintock²,

Barron³

et al. 2009

Experimental and Molecular Pathology

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“…As PT mirrors the function of the extrinsic coagulation pathway and synergy of the vitamin K-dependent coagulation factors, our finding suggests an activation of the extrinsic coagulation pathway in gestational diabetes. A crucial role of sympathetic nervous system in liver-born coagulation and, in particular, extrinsic pathway, is suggested in animal studies [19,20] and also in humans [21]. However, correlation between noradrenaline and PT was absent in our study.…”

Section: Discussioncontrasting

confidence: 61%

Alterations of sympathetic nervous system, coagulation and platelet function in gestational diabetes

Pöyhönen‐Alho

Joutsi‐Korhonen²,

Lassila³

et al. 2012

Blood Coagulation &Amp; Fibrinolysis

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Gestational diabetes is associated with increased risk for venous thromboembolism. Activation of sympathetic nervous system affects blood coagulation, fibrinolysis and platelet activation by several mechanisms. We aimed to study the relationship of sympathetic nervous system, coagulation and platelet function in gestational diabetes. Forty-one white women with gestational diabetes, 22 healthy pregnant and 14 nonpregnant controls were studied. We assayed serial nocturnal (at 12 p.m., 4 a.m. and 7 a.m.) changes of the adrenergic transmitter noradrenaline, coagulation variables and platelet activation with PFA-100. Plasma noradrenaline increased from 4 to 7 a.m. in both pregnant groups. During the same time period, prothrombin time (PT) shortened in gestational diabetes compared with healthy pregnant and nonpregnant controls. In gestational diabetes, nocturnal FVIII:C levels were lower compared with normal pregnancy and also variables associated with von Willebrand factor tended to be lower. Platelet activity increased at midnight in pregnant women compared with nonpregnant women without differences between gestational diabetes and normal pregnancy. Gestational diabetes is associated with concomitant early morning sympathetic stimulation and activation of extrinsic coagulation pathway (shortened PT). Decreased FVIII:C may refer to compensatory anticoagulatory mechanism. These alterations could reflect increased risk of pregnancy-related thromboembolism in gestational diabetes.

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“…1). Administration of a single intraportal injection of 6-OHDA, which induces degeneration of dopaminergic terminal fibers [63, 64], blunts the cholangiocyte functional and proliferative responses to cholestasis, and induces cell death by apoptosis [34]. Chronic administration of clenbuterol (a beta-2 adrenergic agonist) [65] and dobutamine (a beta-1 adrenergic agonist) [66] prevents the decrease in cAMP levels and secretion induced by 6-OHDA, maintains cholangiocyte proliferation, and decreases cholangiocyte apoptosis because of 6-OHDA [34].…”

Section: Neuropeptides and Neurotransmitters Affecting Cholangiocyte mentioning

confidence: 99%

Control of Cholangiocyte Adaptive Responses by Visceral Hormones and Neuropeptides

Marzioni

Fava

Alvaro³

et al. 2008

Clinic Rev Allerg Immunol

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Cholangiocytes, the epithelial cells lining the biliary tree, are the target cells in several liver diseases, termed cholangiopathies. Cholangiopathies are a challenge for clinicians and an enigma for scientists, as the pathogenetic mechanisms by which they develop, and the therapeutic tools for these diseases are still undefined. Several studies demonstrate that many visceral hormones, neuropeptides, and neurotransmitters modulate the adaptive changes of cholangiocytes to chronic cholestatic injury. The aim of this review is to present the recent findings that contributed to clarify the role of visceral hormones and neuropeptides in the regulation of the pathophysiology of cholestasis. These studies helped to shed light on some aspects of cholangiocyte pathophysiology, revealing novel perspectives for the clinical managements of cholangiopathies.

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The Effect of Intraportal Six-hydroxy-dopamine on Hemorrhage after Standardized Liver Trauma in Rats

Cited by 6 publications

References 0 publications

Hemostatic properties of a venomic protein in rat organ trauma

Hemostatic properties of a venomic protein in rat organ trauma

Alterations of sympathetic nervous system, coagulation and platelet function in gestational diabetes

Control of Cholangiocyte Adaptive Responses by Visceral Hormones and Neuropeptides

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