The Effect of Intravitreal N-Methyl-dl-Aspartic Acid on the Electroretinogram in Royal College of Surgeons Rats

Ohzeki, Takayuki; Machida, Shigeki; Takahashi, Tomomi; Ohtaka, Koji; Kurosaka, Daijiro

doi:10.1007/s10384-007-0420-y

Cited by 13 publications

(4 citation statements)

References 43 publications

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“…43 We have already shown that the augmented responses of the inner retina in RCS rats were NMDA-sensitive. [30][31][32] Interestingly, after the elimination of the all postsynaptic responses, the P1 and N2 cone responses were larger in the Tg rabbits. Single rod photoreceptor recordings demonstrate that the recovery phase of the response was delayed in Tg pigs with P347L rhodopsin.…”

Section: Discussionmentioning

confidence: 95%

“…29 It was shown that in RCS rats the inner retinal responses in the cone and rod pathways are preserved despite the progressive loss of the b wave. [30][31][32] In the pig Pro347Leu rhodopsin mutation photoreceptor degeneration model, a failure in the maturation of the cone circuitry has been reported. 33 All of these fi ndings suggest that the signaling in the middle and inner retina is altered in eyes with photoreceptor degeneration.…”

Section: Discussionmentioning

confidence: 99%

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Pharmacological dissection of multifocal electroretinograms of rabbits with Pro347Leu rhodopsin mutation

et al. 2010

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Pharmacological dissection of multifocal electroretinograms of rabbits with Pro347Leu rhodopsin mutation

et al. 2010

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“…It significantly slows the time course of the OFF response and tends to speed up the time course of the ON response. This indicates that ethanol has overall [24,25,28,32] and mammalian b-wave [33][34][35]. The enhancement of the b-wave amplitude caused by NMDA has been attributed to disruption of inhibitory pathways from proximal neurons to ON bipolars cells, because b-wave enhancement is reversed after blockade of GABAergic and glycinergic retinal synapses [28].…”

Section: Discussionmentioning

confidence: 99%

Opposite effects of ethanol on electroretinographic ON and OFF responses are due to its action in the distal retina

Kuzeva¹,

Panev²,

Zhekova³

et al. 2015

J Psychiatry Brain Funct

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The aim of the present study was to give insight into the mechanisms of action of ethanol on the function of retinal ON and OFF channels. In order to fulfill this aim the effects of ethanol on the electroretinographic (ERG) ON (b-wave) and OFF (d-wave) responses were investigated in intact frog eyecups and in eyecups, where the activity of proximal retinal neurons was blocked by 1 mM N-methyl-D-aspartate (NMDA). Ethanol at all concentrations tested (0.25%, 0.5% and 1%) increased the b-wave amplitude and diminished the d-wave amplitude in the intact eyecups. The time characteristics of the d-wave, but not the b-wave were slowed. Perfusion with NMDA alone also caused significant enhancement of the b-wave amplitude and diminution of the d-wave amplitude. When ethanol was applied in combination with NMDA, its effects on both the band d-waves were fully preserved. The results obtained indicate that ethanol effects on the amplitude and time course of the ERG band d-waves are due to its action in the distal frog retina. A hypothesis is presented that the opposite effects of ethanol on the band d-wave amplitude are due to one and the same mechanism-inhibition of the ionotropic non-NMDA glutamate receptors in retinal horizontal and OFF bipolar cells.

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“…All measurements were performed by two independent observers and the average numerical values were taken as the final estimate. The morphometric measurements were used to calculate the fractional thickness of GCL within IR and the number of nuclei per 100 μm 2 of IR ( Takahata et al, 2003 ; Ohzeki et al, 2007 ; Razali et al, 2015 ; Razali et al, 2016 ; Lambuk et al, 2017 ; Mohd Lazaldin et al, 2018 ).…”

Section: Methodsmentioning

confidence: 99%

Neuroprotection Against NMDA-Induced Retinal Damage by Philanthotoxin-343 Involves Reduced Nitrosative Stress

et al. 2021

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N-methyl-D-aspartate receptor (NMDAR) overstimulation is known to mediate neurodegeneration, and hence represents a relevant therapeutic target for neurodegenerative disorders including glaucoma. This study examined the neuroprotective effects of philanthotoxin (PhTX)-343 against NMDA-induced retinal injury in rats. Male Sprague Dawley rats were divided into three groups; group 1 received phosphate buffer saline as the negative control, group 2 was injected with NMDA (160 nM) to induce retinal excitotoxic injury, and group 3 was pre-treated with PhTX-343 (160 nM) 24 h before NMDA exposure. All treatments were given intravitreally and bilaterally. Seven days post-treatment, rats were subjected to visual behaviour assessments using open field and colour recognition tests. Rats were then euthanized, and the retinas were harvested and subjected to haematoxylin and eosin (H&E) staining for morphometric analysis and 3-nitrotyrosine (3-NT) ELISA protocol as the nitrosative stress biomarker. PhTX-343 treatment prior to NMDA exposure improved the ability of rats to recognize visual cues and preserved visual functions (i.e., recognition of objects with different colours). Morphological examination of retinal tissues showed that the fractional ganglion cell layer thickness within the inner retina (IR) in the PhTX-343 treated group was greater by 1.28-fold as compared to NMDA-treated rats (p < 0.05) and was comparable to control rats (p > 0.05). Additionally, the number of retinal cell nuclei/100 μm2 in IR for the PhTX-343-treated group was greater by 1.82-fold compared to NMDA-treated rats (p < 0.05) and was comparable to control group (p > 0.05). PhTX-343 also reduced the retinal 3-NT levels by 1.74-fold compared to NMDA-treated rats (p < 0.05). In conclusion, PhTX-343 pretreatment protects against NMDA-induced retinal morphological changes and visual impairment by suppressing nitrosative stress as reflected by the reduced retinal 3-NT level.

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The Effect of Intravitreal N-Methyl-dl-Aspartic Acid on the Electroretinogram in Royal College of Surgeons Rats

Abstract: With advancing photoreceptor degeneration, the third-order neuronal response made a greater contribution to shaping the a- and b-waves in dystrophic RCS rats.

Cited by 13 publications

References 43 publications

Pharmacological dissection of multifocal electroretinograms of rabbits with Pro347Leu rhodopsin mutation

Pharmacological dissection of multifocal electroretinograms of rabbits with Pro347Leu rhodopsin mutation

Opposite effects of ethanol on electroretinographic ON and OFF responses are due to its action in the distal retina

Neuroprotection Against NMDA-Induced Retinal Damage by Philanthotoxin-343 Involves Reduced Nitrosative Stress

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