The effect of iron on Streptococcus mutans biofilm and on enamel demineralization

Ribeiro, Cecília Cláudia Costa; Ccahuana-Vásquez, Renzo Alberto; Carmo, Cadidja Dayane Sousa do; Alves, Cláudia Maria Coêlho; Leitão, Tarcísio Jorge; Vidotti, Lisandra Rocha; Cury, Jaime Aparecido

doi:10.1590/s1806-83242012000400003

Cited by 30 publications

(29 citation statements)

References 19 publications

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“…Ribeiro et al 10 in their in situ study, stated that iron (Fe) at a concentration of 100µg/ml was able to reduce the number of S. mutans cells present in dental biofilms. 10 Fe has an antibacterial effect not only in terms of killing S. mutans cells, but also by disrupting the ability of these bacteria to form biofilms. It has been shown in situ that dental biofilms formed in humans exposed to Fe contain a lower number of S. mutans.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, by interfering with sucrose metabolism, Fe can reduce the production of EPS. 10 Protein expression in biofilm formation inside the oral cavity is influenced by daily nutrient intake from the food consumed. The analysis above suggests that each inducer produces protein bands of differing molecular weights and densities which differ from those of plankton and from other inducers.…”

Section: Discussionmentioning

confidence: 99%

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Density of Streptococcus mutans biofilm protein induced by glucose, lactose, soy protein and iron

2019

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Background:Caries constitute an infectious disease that result from the interaction of bacteria with the host and the oral environment. Streptococcus mutans (S. mutans) represents the main bacterium that causes caries. The ability of S. mutans to form biofilms in the oral cavity is influenced by daily nutrient intake. This study of bacterial biofilm proteins can be used in the manufacture of kits for the detection of infectious diseases such as caries in the oral cavity. A biomarker is required for the manufacture of the detection kit. Consequently, research must first be conducted to determine the molecular weight and density of S. mutans biofilm proteins induced by several different daily nutrients, namely; 5% glucose, 5% lactose, soy protein and 5% iron. Purpose: This study aimed to analyse the density of S. mutans biofilm protein induced by 5% glucose, 5% lactose, soy protein, and 5% iron. Methods: The density of the S. mutans biofilm protein bands induced were measured using EZ Imager Gel Doc TM software. Results: A band of biofilm protein (61.7 kDa) was obtained from S. mutans induced by 5% glucose, four bands of biofilm protein (180 kDa; 153,9 kDa; 43,9 kDa; 37,5 kDa) from 5% lactose induction and seven bands of biofilm protein (157,9 kDa; 86,6 kDa; 66,5 kDa; 50,1 kDa; 37,9 kDa; 32,3 kDa; 29,4 kDa) from soy protein induction. In contrast, S. mutans induced by 5% iron did not show any protein bands. The proteins that result from each inducer are of differing densities. Conclusion: The protein bands from each inducer are of different densities which can be used in the further test to make a biomarker for dental caries detection kits.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Density of Streptococcus mutans biofilm protein induced by glucose, lactose, soy protein and iron

2019

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“…The model comprises a mature biofilm adhered on enamel, which appears to be a more realistic approach to test the effect on caries than the use of planktonic bacteria or salivary counting used in other studies of probiotics. The model was developed and validated using chlorhexidine to study antimicrobial agents [Ccahuana-Vásquez and Cury, 2010] and used to test several variables such as iron [Ribeiro et al, 2012] and dietary products Muñoz-Sandoval et al, 2012;. The model also demonstrated that the relative Mean absorbance (OD) values at 600 nm, indicating % of coaggregation.…”

Section: Discussionmentioning

confidence: 99%

Effect of the Probiotic Lactobacillus rhamnosus LB21 on the Cariogenicity of Streptococcus mutans UA159 in a Dual-Species Biofilm Model

et al. 2015

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Despite promising results using probiotics, evidence of the preventive effect on enamel demineralization is insufficient and the cariogenic potential of probiotics is still controversial. Probiotics could affect biofilm formation and interfere with adherence, growth or coaggregation with Streptococcus mutans in biofilms. However, most of the studies have been conducted using planktonic bacteria. Hence, the aim of the study was to assess the effect of probiotic bacteria on the cariogenicity of S. mutans using an in vitro biofilm caries model on enamel. Single-species biofilms (S. mutans UA159, SM or Lactobacillus rhamnosus LB21, LB) or dual-species biofilms simultaneously inoculated (SM + LB) or LB inoculated 8 h after SM (SM → LB) were grown for 96 h. Biofilms were formed on bovine enamel saliva-coated slabs of known surface hardness (SH) and immersed in culture media. Biofilms were exposed 8 times per day to 10% sucrose. Medium pH was monitored twice daily as a biofilm acidogenicity indicator. After 96 h, biofilms were collected to determine biomass and bacteria viability. Slab demineralization was calculated as percentage of SH loss (%SHL). Additionally, the model was tested with different concentrations of the initial inoculum (10³, 10⁶, 10⁸ cells/ml) and different adhesion times (2 or 8 h). The dual-species biofilm revealed no LB effects on SM cariogenicity, without changes in acidogenicity or %SHL among groups (p > 0.05, n = 12). Lack of activity of LB on SM cariogenicity persisted even when 10⁵ times higher concentration of the probiotic was tested. Coaggregation was not observed. In conclusion, findings suggest that LB does not reduce cariogenicity of SM in a validated experimental caries model.

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“…It is possible that micronutrients are protective elements against the occurrence of dental caries by contributing to the formation of tooth enamel or by protecting the enamel from bacterial effects [7,26,27]. This effect is plausible, since micronutrient intake at 12 months of age may reflect intake in the previous months, when the primary molars are undergoing the process of calcification.…”

Section: Discussionmentioning

confidence: 99%

No Impact of Lower Intake of Micronutrients on Severe Early Childhood Caries: Findings from a Prospective Cohort Study

Feldens¹,

Kramer²,

Cascaes³

et al. 2015

Pesqui. bras. odontopediatria clín. integr.

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Objective: To investigate the association between micronutrients intake at 12 months of age and the occurrence of severe early childhood caries (S-ECC) at four years of age among children in southern Brazil. Material and Methods: The baseline sample was made up of 500 mother-child pairs followed since the birth of the child in São Leopoldo, Brazil. After the first birthday, micronutrients intake (calcium, iron, sodium, zinc, vitamin A, vitamin C, vitamin B12 and folate) was recorded using the 24-hour recall method. At four years of age, a calibrated examiner evaluated the occurrence of S-ECC based on internationally accepted criteria. Poisson regression was used to investigate associations between exposures and the outcome. Results: The final sample comprised 314 children. The occurrence of S-ECC was higher among children who consumed less calcium (p=0.009), zinc (p=0.021) and vitamin C (p=0.036). However, after the multivariable adjustments, no micronutrient was associated with the occurrence of S-ECC. Conclusion: A lower intake of micronutrients at 12 months of age did not represent a risk factor for the occurrence of S-ECC at four years of age, suggesting that advice on feeding practices for dental caries prevention should focus mainly on dietary aspects (local effect) rather than nutritional aspects (systemic effect).

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The effect of iron on Streptococcus mutans biofilm and on enamel demineralization

Cited by 30 publications

References 19 publications

Density of Streptococcus mutans biofilm protein induced by glucose, lactose, soy protein and iron

Density of Streptococcus mutans biofilm protein induced by glucose, lactose, soy protein and iron

Effect of the Probiotic <b><i>Lactobacillus rhamnosus</i></b> LB21<b> </b>on the Cariogenicity of <b><i>Streptococcus mutans</i></b> UA159 in a Dual-Species Biofilm Model

No Impact of Lower Intake of Micronutrients on Severe Early Childhood Caries: Findings from a Prospective Cohort Study

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