The effect of isosaponarin isolated from wasabi leaf on collagen synthesis in human fibroblasts and its underlying mechanism

Abstract

Wasabi has been used as an important spice in Japanese foods. The wasabi leaves were used as a cosmetic material, but its biological activities have not yet been examined. We investigated the effect of isosaponarin derived from wasabi leaf on collagen synthesis in human fibroblasts. The production of type I collagen in human fibroblasts was increased with treatment of wasabi leaf extract. Isosaponarin isolated from wasabi leaves belonged to the group of flavone glycoside, and was the key compound in collagen synthesis from the wasabi leaf ingredients. Isosaponarin increased the type I collagen production at the mRNA gene level. The treatment of isosaponarin did not influence the production of transforming growth factor-beta (TGF-beta) protein, but increased the production of TGF-beta type II receptor (TbetaR-II) protein and TbetaR-II mRNA. Prolyl 4-hydroxylase (P4H) protein and P4H mRNA were increased by treatment with isosaponarin. Heat shock protein 47 (HSP47) was not increased by treatment with isosaponarin. These results suggested that isosaponarin increased collagen synthesis in human fibroblasts, caused by up-regulated TbetaR-II and P4H production.

Flavonoid and Phenolic Acids Content and In Vitro Study of the Potential Anti-Aging Properties of Eutrema japonicum (Miq.) Koidz Cultivated in Wasabi Farm Poland

“…Extracts from the leaves and roots of E. japonicum have numerous activities, such as antigenotoxic [13], anti-inflammatory [10,12], antioxidant [10,11], antiatopic dermatitis [14], antiplatelet [15], anticancer [15,16], and anti-obesity properties [17]. Besides value as a functional and regular food [18,19], the extract of the leaves of wasabi is also used as a cosmetic product, and it is included in the International Nomenclature of Cosmetic Ingredients (INCI) list [20,21].…”

The Importance and Usages of Wasabia Species

“…Sonuç olarak, izosaponarin ve Wasabia yaprak esktresinin, kırışıklık önleyici çeşitli kozmetik preparatlarında ve diğer terapötik kullanımlar için potansiyel bir materyal olduğu ileri sürülmüştür. 46…”

Anti-obesity Effects of Wasabi Leaf Extract on Rats Fed a High-fat Diet are Related to Upregulation of mRNA Expression of β3-adrenergic Receptors in Interscapular Brown Adipose Tissue

“…There are some reports regarding the physiological functions of these leaves, including anti-fungal (Pedras et al, 1999), anti-oxidative (Hosoya et al, 2008;Lee et al, 2010;Sekiguchi et al, 2010), anti-cancer (Okamoto et al, 2008), anti-inflammatory , anti-influenza virus (Mochida and Ogawa, 2008), and collagen synthesis (Nagai et al, 2010) activities. In a study identifying compounds besides isothiocyanates in wasabi leaves, polyphenols such as flavonoids (Hosoya et al, 2005), phenylpropanoids (Hosoya et al, 2008), terpenoids , and carotenoids were isolated.…”