The Effect of Jet Parameters on Jet Injection

Wendell, Dawn; Hemond, Brian D.; Hogan, Neville; Taberner, Andrew J.; Hunter, Ian W.

doi:10.1109/iembs.2006.260369

Cited by 20 publications

(4 citation statements)

References 9 publications

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“…The jet injection was first described in 1947 as a needlefree drug delivery method in contrast to the conventional needle injection (37). Jet injection is accomplished through a high-speed, ultrafine stream of DNA solution driven by a pressurized gas, usually CO 2 .…”

Section: Needle and Jet Injectionmentioning

confidence: 99%

Nonviral Gene Delivery: Principle, Limitations, and Recent Progress

Al‐Dosari

Gao

2009

AAPS J

613

438

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Abstract. Gene therapy is becoming a promising therapeutic modality for the treatment of genetic and acquired disorders. Nonviral approaches as alternative gene transfer vehicles to the popular viral vectors have received significant attention because of their favorable properties, including lack of immunogenicity, low toxicity, and potential for tissue specificity. Such approaches have been tested in preclinical studies and human clinical trials over the last decade. Although therapeutic benefit has been demonstrated in animal models, gene delivery efficiency of the nonviral approaches remains to be a key obstacle for clinical applications. This review focuses on existing and emerging concepts of chemical and physical methods for delivery of therapeutic nucleic acid molecules in vivo. The emphasis is placed on discussion about problems associated with current nonviral methods and recent efforts toward refinement of nonviral approaches.

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Section: Needle and Jet Injectionmentioning

confidence: 99%

Nonviral Gene Delivery: Principle, Limitations, and Recent Progress

Al‐Dosari

Gao

2009

AAPS J

613

438

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“…The fat only samples were obtained from pork belly purchased at a local butcher, with the skin and muscle layers carefully removed. All samples were stored in a −80°C freezer and thawed to room temperature (∼22°C) prior to experimentation, a handling procedure consistent with other jet injection studies .…”

Section: Methodsmentioning

confidence: 99%

Spatially resolved diffuse imaging for high‐speed depth estimation of jet injection

Brennan

Ruddy

Nielsen

et al. 2019

Journal of Biophotonics

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We investigate the use of spatially resolved diffuse imaging to track a fluid jet delivered at high speed into skin tissue. A jet injector with a short needle to deliver drugs beneath the dermis, is modified to incorporate a laser beam into the jet, which is ejected into ex vivo porcine tissue. The diffuse light emitted from the side and top of the tissue sample is recorded using high‐speed videography. Similar experiments, using a depth‐controlled fiber optic source, generate a reference dataset. The side light distribution is related to source depth for the controlled‐source experiments and used to track the effective source depth of the injections. Postinjection X‐ray images show agreement between the jet penetration and ultimate light source depth. The surface light intensity profile is parameterized with a single parameter and an exponential function is used to relate this parameter to source depth for the controlled‐source data. This empirical model is then used to estimate the effective source depth from the surface profile of the injection experiments. The depth estimates for injections into fat remain close to the side depth estimates, with a root‐mean‐square error of 1.1 mm, up to a source depth of 8 mm.

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“…The jet injection method of drug delivery has been in practice in medicine since 1947. It is a needle-free method that is capable of inserting and dispersing the therapeutic material or gene in target cells [ 56 ]. This device is capable of creating a very fine jet of genetic material that is delivered to the target site at a very high pressure.…”

Section: Nonviral Vectors For Gene Deliverymentioning

confidence: 99%

Appraisal for the Potential of Viral and Nonviral Vectors in Gene Therapy: A Review

Butt

Zaman

Ahmad

et al. 2022

Genes

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Over the past few decades, gene therapy has gained immense importance in medical research as a promising treatment strategy for diseases such as cancer, AIDS, Alzheimer’s disease, and many genetic disorders. When a gene needs to be delivered to a target cell inside the human body, it has to pass a large number of barriers through the extracellular and intracellular environment. This is why the delivery of naked genes and nucleic acids is highly unfavorable, and gene delivery requires suitable vectors that can carry the gene cargo to the target site and protect it from biological degradation. To date, medical research has come up with two types of gene delivery vectors, which are viral and nonviral vectors. The ability of viruses to protect transgenes from biological degradation and their capability to efficiently cross cellular barriers have allowed gene therapy research to develop new approaches utilizing viruses and their different genomes as vectors for gene delivery. Although viral vectors are very efficient, science has also come up with numerous nonviral systems based on cationic lipids, cationic polymers, and inorganic particles that provide sustainable gene expression without triggering unwanted inflammatory and immune reactions, and that are considered nontoxic. In this review, we discuss in detail the latest data available on all viral and nonviral vectors used in gene delivery. The mechanisms of viral and nonviral vector-based gene delivery are presented, and the advantages and disadvantages of all types of vectors are also given.

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The Effect of Jet Parameters on Jet Injection

Cited by 20 publications

References 9 publications

Nonviral Gene Delivery: Principle, Limitations, and Recent Progress

Nonviral Gene Delivery: Principle, Limitations, and Recent Progress

Spatially resolved diffuse imaging for high‐speed depth estimation of jet injection

Appraisal for the Potential of Viral and Nonviral Vectors in Gene Therapy: A Review

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