The effect of Jiedu Huoxue decoction on rat model of experimental nonbacterial prostatitis via regulation of miRNAs

Abstract

Context: The underlying mechanisms of Jiedu Huoxue decoction (JDHXD) in treating chronic prostatitis have not been fully explored. Objective: This study investigates the miRNAs as potential biomarkers and the effect of JDHXD on the rat model of experimental nonbacterial prostatitis. Materials and methods: Fifty-four Sprague-Dawley male rats were randomly divided into normal control, model, JDHXD low dose (0.5 g/kg/day), medium dose (1 g/kg/day), high dose (2 g/kg/day) and western medicine (cernilton 0.094 g/kg/day) groups, and intragastrically administered once daily for 30 days. The control and model (upon successful establishment) groups received distilled water. Differential expression of miRNAs was analysed with high-throughput miRNA sequencing and validated with qRT-PCR and Northern blot. Prediction of specific target genes and functional enrichment analysis were performed with bioinformatics. Results: LD 50 test showed no sign of toxicity with maximum feasible dose 4 g/kg JDHXD. Compared with control, 495 miRNAs showed expression changes in CAP/CPPS rats, of which 211 were significantly different and 37 were prostatic-related. There were 181 differentially expressed miRNAs between the model and high dose JDHXD groups, of which 23 were identical with the control and model groups. Compared with control, miR-146a, miR-423 and miR-205 expression increased significantly in the model group, decreased dose-dependently in the JDHXD groups (p < 0.05), and vice-versa for miR-96 (p < 0.05). The effect of low dose JDHXD was comparable to cernilton (p > 0.05). Discussion and conclusions: Future studies may explore the contributions of the active components in JDHXD. The study design is generalisable. The effect can be repeatedly verified in clinical trials.

Network Pharmacology and Experimental Verification Revealed the Mechanism of Yiqi Jianpi Recipe on Chronic Obstructive Pulmonary Disease

“…The YQJPR group continued the TCM intervention after the 12th week of modelling, and the whole TCM intervention lasted for 12 weeks. The dose of YQJPR to rats was calculated from the dose conversion method between humans and rats [ 15 ]. The adult clinical dosage of YQJPR was 1.786 g/(kg·d), and then, the YQJPR group was given 11.25 g/(kg·d) by gavage, while the control and model groups were given the same amount of normal saline by gavage.…”

“…e YQJPR group continued the TCM intervention after the 12th week of modelling, and the whole TCM intervention lasted for 12 weeks. e dose of YQJPR to rats was calculated from the dose conversion method between humans and rats [15].…”

The potential mechanism of huazhuojiedu decoction in the treatment of ulcerative colitis based on network pharmacology and experimental validation

“…The adult dose of HZJDD was 192 g/d. By calculating the dose conversion coefficient per kilogram of body weight between animals and humans (Phok et al, 2017;Du et al, 2021), when the adult standard body weight is 60 kg, the equivalent dose of HZJDD in rats was about 20 g/kg/d, so the doses in this study were 10, 20, and 40 g/kg/d respectively (Yan et al, 2020).…”

Clinical Significance of miR-21-5p in Predicting Occurrence and Progression of Uremic Vascular Calcification in Patients with End-Stage Renal Disease

“…MicroRNAs (miRNAs), a class of small RNA molecules of 17–25 bp in length, play a crucial role in the mediation of gene expression. 5 As non-traumatic biomarkers, miRNAs have been reported to be stably expressed in the blood and dysregulated in various diseases. 6 With advancing research, more miRNAs have been found to be aberrantly expressed in metabolic and cardiovascular diseases: 7 For example, miR-33 and miR-34a appear to be involved in the metabolism of fat, sugar, and cholesterol in the liver.…”