The effect of K+ on the membrane potential in HeLa cells

Okada, Yasunobu; Ogawa, Michio; Aoki, Natsue; Izutsu, Kosaku

doi:10.1016/0005-2736(73)90066-7

Cited by 28 publications

(22 citation statements)

References 22 publications

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“…For electrophysiological studies, the cells were plated on the glass bottom (the cover slip) of a glass chamber such as was used previously (Okada, Ogawa, Aoki & Izutsu, 1973). For electrophysiological studies, the cells were plated on the glass bottom (the cover slip) of a glass chamber such as was used previously (Okada, Ogawa, Aoki & Izutsu, 1973).…”

Section: Methodsmentioning

confidence: 99%

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Oscillations of membrane potential in L cells

et al. 1977

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The membrane potentials and resistances of L cells were measured using a standard electrophysiological technique. The values obtained in physiological media were around--15 mV and 37Momega, respectively. Almost all the large nondividing L cells (giant L cells) showed spontaneous oscillations of the membrane potential between around -15 and -40 mV. Application of an appropriate electrical or mechanical stimulus was also capable of eliciting responses but such were usually induced only once. The total membrane conductance increased significantly and in parallel with such a hyperpolarizing response. Cooling of the cells and application of metabolic inhibitors to the cells completely blocked the spontaneous oscillation despite the fact that the electrically induced hyperpolarizing response remained. Intracellular K+, Na+ and Cl- concentrations were measured by means of a flame photometer and a chloridometer, and the equilibrium potential for each ion was estimated.

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Section: Methodsmentioning

confidence: 99%

“…All the electrophysiological measurements were made on an inverted phase microscope (Chiyoda Type T-2) under a magnification of 1000x or 600 x as described previously (Okada et al, 1973). To maintain the temperature of the cell chamber, the temperature of the microscope stage was kept at 37_+ 1 ~ by circulating warm water with a pump.…”

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confidence: 99%

Oscillations of membrane potential in L cells

et al. 1977

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“…The effect of high extracellular (K +) on the PNa/PK of membranes has been studied in HeLa cells (Okada, 1973) and the mucosal membrane of rat duodenum (Okada, Toshinori & Inouye, 1975). For both of these preparations PNa/PK decreased as the extracellular (K +) increased.…”

Section: Relative Permeability Of the Basolateral Membranementioning

confidence: 98%

Basolateral membrane potential of a tight epithelium: Ionic diffusion and electrogenic pumps

1978

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The contribution of specific ions to the conductance and potential of the basolateral membrane of the rabbit urinary bladder has been studied with both conventional and ion-specific microelectrode techniques. In addition, the possibility of an electrogenic active transport process located at the basolateral membrane was studied using the polyene antibiotic nystatin. The effect of ion-specific microelectrode impalement damage on intracellular ion activities was examined and a criterion set for acceptance or rejection of intracellular activity measurements. Using this criterion, we found (K+) = 72 mM and (Cl-) = 15.8 mM. Cl- but not K+ was in electrochemical equilibrium across the basolateral membrane. The selective permeability of the basolateral membrane was measured using microelectrodes, and the data analyzed using the Goldman, Hodgkin-Katz equation. The sodium to potassium permeability ratio (PNa/PK) was 0.044, and the chloride to potassium permeability ratio (PCl/PK) was 1.17. Since K+ was not in electrochemical equilibrium, intracellular (K+) is maintained by active metabolic processes, and the basolateral membrane potential is a diffusion potential with K+and C1- the most permeable ions. After depolarizing the basolateral membrane with high serosal potassium bathing solutions and eliminating the apical membrane as a rate limiting step for ion movement using the polyene antibiotic nystatin, we found that the addition of equal aliquots of NaCl to both solutions caused the basolateral membrane potential to hyperpolarize by up to 20mV (cell interior negative). This potential was reduced by 80% within 3 min of the addition of ouabain to the serosal solution. This hyperpolarization most probably represents a ouabain sensitive active transport process sensitive to intracellular Na+. An equivalent electrical circuit for Na+ transport across rabbit urinary bladder is derived, tested, and compared to previous results. This circuit is also used to predict the effects that microelectrode impalement damage will have on individual membrane potentials as well as time-dependent phenomena; e.g., effect of amiloride on apical and basolateral membrane potentials.

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“…Therefore, the readings were different from the membrane potential determined with microelectrode. [31] However, it is interesting to find that a much bigger potential decrease (≈−43 mV) was observed when the GPS directly penetrated into the nucleus of the cell (arrow 1 in the blue curve). When the GPS penetrated through the nucleus (arrow 2 in the blue curve) and cytomembrane (arrow 3 in the blue curve), the potential increased back to about −8 to −6 mV in the cytoplasm and 0 mV in buffer, respectively.…”

Section: Localized Ph Probing In Single Cellsmentioning

confidence: 99%

A Multiparameter pH‐Sensitive Nanodevice Based on Plasmonic Nanopores

Liu

Jiang

Pang

et al. 2017

Adv Funct Materials

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With controllable mass transfer and special optical properties, plasmonic nanopores may be applied as a nanodevice and possibly create a new generation of single molecule detection technique based on plasmon-enhanced spectra. In the present study, gold nanoparticles self-assemble into a gold porous sphere (GPS) on the tip of a glass nanopipette with the help of i-motif DNA thiolated by both ends as linker molecules. The gaps among neighboring gold nanoparticles are considered as plasmonic nanopores. The size of the formed nanopores can be tuned by the folded-unfolded conformational change of i-motif DNA upon pH adjustment from 4.5 to 7.0. Based on its tunable structural property, the GPS shows reversible changes in ionic current, potential, and surface-enhanced Raman scattering signal. The GPS is further used to probe regional pH in single cells. The successful application of GPS in multiparameter pH probing and single cell analysis suggests that the new physical properties of the self-assembled plasmonic nanopores can be used for fabricating multiple types of nanodevices and nanosensors.

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The effect of K+ on the membrane potential in HeLa cells

Cited by 28 publications

References 22 publications

Oscillations of membrane potential in L cells

Oscillations of membrane potential in L cells

Basolateral membrane potential of a tight epithelium: Ionic diffusion and electrogenic pumps

A Multiparameter pH‐Sensitive Nanodevice Based on Plasmonic Nanopores

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