The effect of Kalimantan’s honey propolis toward the quality of mice’s (mus musculus l.) spermatozoa that exposed by cigarette smoke

Abstract

Propolis contains flavonoids that act as antioxidants beneficial in preventing cell damage from oxidative stress. This study aimed to determine the effect of Kalimantan's honey propolis toward motility and viability of male mice spermatozoa (Mus musculus L.) that exposed by cigarette smoke. The study subjects using 25 male mice Swiss strain were divided randomly into 5 groups. The division of the group consisting of: normal control (given Aquadest), negative controls (treated by 1 cigarette per day), treatment 1 (treated by one cigarette + @ 0.1 ml of extract of propolis), treatment 2) treated by 1 cigarette + @ 0 , 2 ml of extract of propolis), treatment 3 (treated by 1 cigarette + @ 0.4 ml propolis extract). Treatment lasted for 47 days, on day 48 mice in termination, then examined the quality of motility and viability of sperm that. Average results obtained in mice sperm motility negative controls (13.20%), normal controls (46.34%), treatment 1 (26.56%), treatment 2 (30.16%), and treatment 3 (33.60 %). The results mean viability of spermatozoa of mice obtained in the negative control (21.20%), normal controls (31.86%), treatment 1 (48.40%), treatment 2 (48.56%), and treatment 3 (53.00 %). Test results one way ANOVA there are significant differences in the quality of sperm is sperm motility and viability of spermatozoa (P <0.05). The Kalimantan's honey propolis may improve sperm quality of mice (mus musculus L.) after exposed by cigarette smoke.