The effect of Kappaphycus alvarezii fraction on plasma glucose, Advanced Glycation End-products formation, and renal RAGE gene expression

The effect of Kappaphycus alvarezii active fraction on oxidative stress and inflammation in streptozotocin and nicotinamide-induced diabetic rats

“…Plant determination was carried out at the Basic Biology Laboratory, Faculty of Mathematics and Natural Sciences, Mataram University, Indonesia, with a Certificate of Identification no 03/UN18.7/LB/2019. Active fraction used in this study was obtained from previous study [ 14 ]. Wistar male rats (200 g, aged 8 weeks) were obtained from Bogor Life Science and Technology (BLST) Company, Bogor, Indonesia.…”

“…The active fraction from K. alvarezii were obtained from a previous study [ 14 ] using bioassay guided fractionation. Previous studies have succeeded in separating four fractions through the bioassay guided fractionation method (fraction I-IV), which we then selected the most active fraction as the active fraction (fraction II).…”

“…Previous study using diabetic rats has shown that the active fraction from this algae has antiglycation properties by reducing AGEs, namely glycated albumin (GA) and Nε- (carboxymethyl) lysine (CML) [ 14 ]. Phytochemicals inhibit the formation of AGEs through various pathways, including lowering glucose levels, inhibiting aldose reductase, metal chelation activity, hypolipidemic activity, free radicals scavenging, reducing AGEs cross-links, competing with reducing sugars to bind to proteins, and inhibiting TNF, which is involved in insulin resistance.…”

Piper crocatum Ruiz & Pav. ameliorates wound healing through p53, E-cadherin and SOD1 pathways on wounded hyperglycemia fibroblasts

“…Bioassay-guided fractionation was performed in our study to identify the active fractions through three steps (extraction, partition, and fractionation). This method was successful in separating the active compound groups in Tithonia Diversifolia and Kappaphycus alvarezii (Doty) Doty ex P.C.Silva algae ( Syarif et al, 2018 , Yulianti et al, 2021 ). One additional step from this method can be used to isolate the active compounds contained in natural materials, for example the Tagitinin C compound from Tithonia diversifolia ( Wahyuningsih et al, 2015 ) and zerumbone from Zingiber zerumbet ( Murini et al, 2018 ).…”

“…TLC identification of liquid chromatography yielded 12 fractions that were combined into four fractions, i.e., fraction I (F I ), fraction II (F II ), fraction III (F III ), and fraction IV (F IV ) based on spot similarity, then used for the pro-healing tests. The active fraction that had the highest percentage of proliferation from the MTT assay was then used for further testing ( Yulianti et al, 2021 ).…”