The Effect of Kebar Grass Extract (Biophytum petersianum Klotzch) on Total Growth of Ovarian Follicles on Mice (Mus Musculus) during Lactation period were Exposed by Carbofuran

Abstract

The study aimed to know the effect of Kebar grass extraction amounts of mice (Mus musculus) follicles ovary exposed by carbofuran. This study was an experimental study with a completely randomized design. Forty two lactation mice used in this study were divided into seven groups, that were: C was control (Aquadest), P1 was injected with carbofuran 1/4 LD50 (0.0125mg/day), P2 was injected with carbofuran 1/8 LD50 (0.00625mg/day), P3 was injected with carbofuran 1/4 LD50 (0.0125mg/day) and Kebar grass extract (0.135mg/day), P4 was injected with carbofuran 1/8 LD50 (0.00625mg/day) and Kebar grass extract (0.135mg/day), P5 was injected with carbofuran 1/4 LD50 (0.0125mg/day) and vitamin C (0.2ml/day) and P6 was injected with carbofuran 1/8 LD50 (0.00625mg/day) and vitamin C (0.2ml/day) with a treatment period of 14 days. Then on the 15day of treatment, mice were sacrificed, and the ovary was taken. Then, histology preparation with HE (Hematoxylin-eosin) staining was made, and follicles ovary were counted. Data were analyzed by One Way ANOVA followed by the Duncan test. This study showed that exposed carbofuran was a significant decrease in the growth of ovarian follicles (p<0.05). The administration of the Kebar grass extraction exposure to carbofuran is proven to increase primary and secondary follicles. The Kebar grass extraction administration gave a better effect than vitamin C (p<0.05).

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