The Effect of L-Arginine on Growth Performance, Some Serum Biochemical Parameters and Duodenal Motility in Broilers

Abstract

The aim of this study was to evaluate the effect of diet supplemented with L-arginine (L-Arg)on growth performance, some serum biochemical parameters and duodenal motility of broilers during three time periods: 0 to 10, 11 to 28 and 29 to 42 days old. A total of 500, mixed sex, one-dayold Ross-308 broiler chicks were divided into five groups as follows: Arg deficient group and four experimental groups. Each group was then divided into five subgroups of 20 chicks each. Arg deficient group for all time periods was fed by basal diet which contained 10% less L-Arg than optimum Arg requirement recommended by the breeder. Experimental groups were fed by basal diet supplemented with L-Arg which was progressively 10% increased in groups. The highest body weight gain (BWG) was observed on days 11-28 and 0-42 in experimental group fed by basal diet supplemented with 110% L-Arg, whereas the lowest feed conversion ratio (FCR) was determined on days 29-42 and 0-42 in the same experimental group. Feed intake did not change in all three periods, while serum urea nitrogen level in the experimental group in which diet supplemented with 10% L-Arg, was lower than other groups on day 0-10. On contractility studies, it was observed that L-Arg inhibited the amplitude of contractions in duodenum in a dosedependent manner in vitro. These results suggest that the basal diet formulated with 90-130% Arg is not effective on growth performance of chicks on days 0-10, whereas the diet supplemented with 10% L-Arg more than optimum Arg requirement is adequate during the days 11-28 and 29-42. Moreover, although the L-Arg decreased the duodenal contractility in vitro, it is suggested that the diet supplemented with 10% L-Arg more than optimum Arg requirement may be negatively affected the FCR in broilers. Keywords: Broiler, L-arginine, Growth performance, Duodenal contractility L-Arjininin Broylerlerde Büyüme Performansı, Bazı Biyokimyasal Parametreler ve Duodenal Motilite Üzerine Etkisi ÖzetBu araştırma 0-10., 11-28. ve 29-42. günler arasında broylerlerde L-arjinin (L-Arj)nin rasyonlara katılmasının büyüme performansı, bazı biyokimyasal parametreler ve duodenal motilite üzerine etkisini belirlemek amacıyla yapılmıştır. Araştırmada toplam 500 adet, bir günlük yaşta, karışık cinsiyette Ross 308 broyler civciv: Arj yetersiz grup ve dört deneme grubu olmak üzere beş gruba ayrılmıştır. Her bir grup da 20'şer civcivden oluşan 5 alt gruba ayrılmıştır. Arjinince yetersiz grup tüm deneme boyunca üretici tarafından önerilen optimum Arj gereksiniminin %10'undan düşük L-Arj içeren temel rasyon ile beslenmiştir. Deneme grupları ise, temel rasyona %10 progresif artan düzeylerde L-Arj ilave edilen rasyonlarla beslenmiştir. Araştırma sonunda ihtiyacın %110 düzeyinde Arj bulunan diyetle beslenen grupta 11-28 ve 0-42. (P<0.01) günlerde en yüksek canlı ağırlık artışı (CAA), buna karşın aynı deneme grubunda 29-42. ve 0-42. günlerde en düşük yemden yararlanma oranı (YYO) bulunmuştur. Her üç dönemde YT gruplarda değişmezken serum üre azot düzeyi optimum Arj gereksin...

Effects of dietary supplementation of arginine and lysine on performance and egg quality characteristics of laying quails

“…Several studies conducted with broilers (6,19,21,34) reported that arginine and lysine supplementation affected BW gain positively, while it was decreased by arginine and lysine deficiency (14,20,22). On the other hand, excessive arginine and lysine supplementation in the diet (twice the level of requirement) has been reported to lower BW gain for chicks (7) or keep it steady for chickens (2) and chicks (4,32). Other studies reported that arginine and lysine supplementation had positive effects only under stressful circumstances (33); the requirement changed depending on the protein level of the diet (17,24).…”

“…The eggs were 3 The arginine and lysine levels of the basal diet were determined by LC-MS-MS (Applied Biosystems API-3200, ANT Technical Devices Lab.) 4 The methionine and methionine+cystine levels of the basal diet were calculated according to the Batal and Dale (3) examined for weight (g), length (mm), width (mm), eggshell thickness (mm), fracture strength, albumen index (%), yolk index (%), Haugh unit, egg shape index and yolk color index. Egg width, egg length, yolk width, albumen length and albumen width were measured by compass to the nearest 0.01 mm.…”

“…Body-weight (BW) gain, feed intake (FI) and feed conversion ratio (FCR) in poultry are adversely affected by deficiencies of these amino acids (6,14,20,21). In contrast, excessive levels of these amino acids in the diet have been found to result in impaired growth performance (4,7,8,9,10), and to not have any positive effects on egg production and quality (2).…”

17β-Estradiol Inhibites Nitric Oxide-cGMP-Dependent Pathway but may Activate Independent Pathway in Small Intestinum of Ovariectomized Rat

“…Moreover, L-arginine at around 0.01-1 mM concentrations totally removes the contractile activity of smooth muscles in vitro [26] . It has been clearly showed that the most effective dose of L-arginine is 10 -5 M [18,27] , therefore we preferred to use L-arginine at this dose range. It has been reported that estrogens increase NOS expression in intestine [28] .…”

“…This finding supports our previous suggestion that exogenously produced NO shows its relaxative effect mediated by estrogens and the NO/NOS independent pathway may be associated with this effect. Moreover, SNP generates cGMP-dependent and independent relaxation in smooth muscles [18,27] . In our study, the relaxant activity of L-arginine and 8-BrcGMP was decreased increasing dose-dependent manner of 17β-estradiol, whereas SNP increased the percentage inhibition of smooth muscle contractility (Table 2).…”