The Effect of L-Carnitine, Hypotaurine, and Taurine Supplementation on the Quality of Cryopreserved Chicken Semen

Partyka, Agnieszka; Rodak, Olga; Bajzert, Joanna; Kochan, Joanna; Niżański, Wojciech

doi:10.1155/2017/7279341

Cited by 60 publications

(71 citation statements)

References 55 publications

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“…Reducing the quality of sperm by oxidative shock may result from a series of cascading events that reduce intracellular ATP levels, release various apoptotic agents (such as procaspase, cytochrome C, and apoptosis inducers) through the damaged mitochondria membrane into cytosol, deactivation of some biochemical pathways, failure of the enzymes and increased permeability of the membrane (Fanaei et al, ). Therefore, oxidative stress can initiate apoptosis (Partyka, Rodak, Bajzert, Kochan, & Niżański, ). An oxidative attack on unsaturated fatty acid phospholipid bonds causes a change in fluidity and membrane integrity (Sarıözkan et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…An oxidative attack on unsaturated fatty acid phospholipid bonds causes a change in fluidity and membrane integrity (Sarıözkan et al, ). Although sperm DNA is resistant to most stresses, freezing can cause sperm DNA damage (Partyka et al, ). ROS agents invade the DNA, causing fragmentation and loss of the DNA base structure, separation of the two DNA strands, and damage to DNA integrity.…”

Section: Discussionmentioning

confidence: 99%

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Canthaxanthin protects human sperm parameters during cryopreservation

2019

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Different antioxidants have been introduced to reduce oxidative stress during the cryopreservation. The main goal of this study was to evaluate the effects of canthaxanthin on human sperm parameters during the freeze‐thaw process. This study was performed on 25 normozoospermic semen samples dividing into five groups including 0, 0.1, 1, 10, and 25 µM of canthaxanthin. The prepared spermatozoa were cryopreserved by rapid freezing technique. Sperm motility, viability (eosin‐nigrosin), morphology (Papanicolaou), acrosome reaction (double staining), DNA denaturation (acridine orange), chromatin packaging (aniline blue and toluidine blue), and DNA fragmentation (sperm chromatin dispersion test) were evaluated before freezing and after thawing. All sperm parameters after thawing significantly were decreased compared to before freezing. Twenty‐five micromolar canthaxanthin could significantly improve the progressive and total motility, viability, normal morphology, chromatin packaging, acrosome integrity and DNA denaturation and fragmentation. Ten micromolar canthaxanthin significantly improved total motility, viability, normal morphology, chromatin packaging, acrosome integrity and DNA denaturation and fragmentation. Whereas, in 1 µM group, there were significant differences only in improvement of acrosome integrity, chromatin packaging (toluidine blue) and DNA denaturation and fragmentation. But, in 0.1 µM group, there were no significant differences in any of measured parameters. It seems that canthaxanthin ameliorates detrimental effects of cryopreservation on human sperm parameters.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Canthaxanthin protects human sperm parameters during cryopreservation

2019

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“…The addition of antioxidants can effectively reduce oxidative stress. Adding Vitamin E analog to rabbit spermatozoa (Zhu et al, ), L‐arginine to ram spermatozoa (Özer Kaya, Gür, & Kaya, ) and taurine to chicken spermatozoa (Partyka, Rodak, Bajzert, Kochan, & Niżański, ) have been shown to improve sperm motility and membrane integrity during liquid storage. In this study, supplement with 0.05 and 0.1 mM melatonin significantly improves plasma membrane integrity ( p < 0.05).…”

Section: Discussionmentioning

confidence: 99%

Effect of addition of melatonin on liquid storage of ram semen at 4°C

et al. 2019

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Adding a certain amount of antioxidants to semen extender has been shown to improve semen quality. The aim of present study was to elucidate whether the supplementation of melatonin to the Tris-based extender (CTR) could enhance the quality of ram spermatozoa during storage at 4°C. Ram semen samples were collected and diluted with CTR extender containing different concentrations (0, 0.05 (M 0.05), 0.1 (M 0.1), 0.2 (M 0.2) or 0.4 (M 0.4) mM) of melatonin. Sperm routine indicators, mitochondrial activity, total antioxidant capacity (T-AOC) and malondialdehyde (MDA) content were analysed in control and melatonin treatment groups. The higher per cent of motility, plasma membrane integrity, mitochondrial activity and T-AOC activity was observed in M 0.05, M 0.1 and M 0.2 groups compared to control group at 5 days of storage (p < 0.05), while lower percentage of MDA content was observed among these groups (p < 0.05). In addition, there were no significant differences in acrosome integrity among the control and M 0.05, M 0.1 and M 0.2 groups during the experiment. The above results show that the addition of 0.05, 0.1, 0.2 mM of melatonin is beneficial to the preservation of ram semen during liquid storage at 4°C mainly through antioxidative stress. K E Y W O R D S antioxidant, melatonin, Ram, semen storage How to cite this article: Dai G-C, Meng Y, Zhang L, et al. Effect of addition of melatonin on liquid storage of ram semen at 4°C.

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“…According to Yang et al (2010a) the cryoprotective properties of taurine on ram spermatozoa may be attributed rather to its osmoregulation abilities than to its antioxidative potential. However Partyka et al (2017) demonstrated that substances such as l-carninite and taurine are antioxidants that improve cryopreserved chicken semen. Their study showed that taurine addition showed best results in sperm motility, viability, and mitochondrial activity and reduced spermatozoa apoptosis and DNA damage.…”

Section: Discussionmentioning

confidence: 99%

Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility

Slanina¹,

Miškeje²,

Tirpák³

et al. 2018

Czech J. Anim. Sci.

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Slanina T., Miškeje M., Tirpák F., Błaszczyk M., Stawarz R., Massányi P. (2018): Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility. Czech J. Anim. Sci., 63, 127-135.The effect of taurine on the turkey spermatozoa motility and viability during the in vitro incubation was assessed. Experimental samples were prepared by diluting the raw semen in nine different concentrations of taurine -from 10 mg/ml to 0.078125 mg/ml. The motility parameters were evaluated by the CASA system (Computer Assisted Semen Analyser) using the program Sperm Vision ® and for spermatozoa viability assessment the eosin-nigrosin staining was performed. Selected parameters were evaluated at six time periods: 0, 1, 2, 3, 4, and 5 h at 5°C and 41°C. At 5°C, a significantly lower percentage of motility and progressive motility was detected only in the samples with the highest concentration of taurine (10 mg/ml) at time 0 and 1. After 2 h of incubation a significant preventive effect of taurine on spermatozoa parameters was observed. The tendency of the taurine effect on motility parameters was different during the in vitro incubation at 41°C. Significantly lower values of motility parameters were detected in all experimental samples in comparison to the control after 5 h. The analysed concentrations of taurine did not significantly affect viability of turkey spermatozoa during all time periods. A higher percentage of dead spermatozoa were observed at 41°C (4.87-9.90%) if compared to 5°C (2.12-4.88%). The results indicated that the addition of taurine (from 2.5 to 7.5 mg/ml) to turkey spermatozoa positively affected the monitored spermatozoa parameters incubated at 5°C.

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The Effect of L-Carnitine, Hypotaurine, and Taurine Supplementation on the Quality of Cryopreserved Chicken Semen

Cited by 60 publications

References 55 publications

Canthaxanthin protects human sperm parameters during cryopreservation

Canthaxanthin protects human sperm parameters during cryopreservation

Effect of addition of melatonin on liquid storage of ram semen at 4°C

Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility

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