The effect of Lactobacillus johnsonii Ncc533 (La1) on the balance of Th1/Th2 cells in BALB/c mice

Abstract

e e ect of Lactobacillus johnsonii Ncc533 (La1) on the balance of 1/ 2 cells in BALB/c mice Abstract Purpose: To determine the e ect of Lactobacillus johnsonii Ncc533 (La1) on 1/ 2 balance, the production of IL-4 and IFN-γ by splenocytes was evaluated following its administration to mice from newborn to adult. Changes in IL-4 and IFN-γ expression and serum levels of OVA-speci c-IgE were then investigated in an asthma model. Methods:Using ow cytometry (FCM) and ELISA, the percentage of IL-4 and IFN-γ expressing splenocytes and serum levels of OVA-speci c-IgE were measured in di erent groups of mice.Results: e percentages of IL-4 and IFN-γ expressing splenocytes in the o spring and in the adults of the La1-treated group were not signi cantly di erent when compared with the water-treated group. In the asthma model, the percentages of IL-4 expressing cells and the serum levels of OVA-speci c-IgE in the La1-treated and water-treated group were signi cantly increased compared with those in the control group. e percentage of IFN-γ expressing cells was signi cantly lower in the La1-treated and water-treated groups. e percentage of IL-4 expressing cells and the serum levels of OVA-speci c-IgE in the La1-treated group were signi cantly lower compared with those in the water-treated group, whereas the percentage of IFN-γ expressing cells was signi cantly higher.Conclusion: Administration of La1 had no e ect on the immune system from the neonate to the adult in the normal mice. It did, however, signi cantly alter the percentages of IL-4 or IFN-γ expressing CD4 + T lymphocytes in the asthma model, suggesting that administration of La1 might regulate the immune response.ORIGINAL RESEARCH

Probiotics for Prevention of Atopy and Food Hypersensitivity in Early Childhood

“…Until recently, several observations have challenged this paradigm that the presence of allergic and autoimmune diseases are mutually exclusive states, and provided additional insight into the roles of mast cells, B cells, and some other subsets of T cells (such as, Th17 and regulatory T cells) as a common link between atopy and autoimmunity. 61 There is growing body of evidence indicating that probiotics could modulate the immune response, such as the balance of Th1/Th2 cells, 62 and prevent autoimmune and allergic diseases. 5 , 12 , 63 Still, the exact mechanisms up to now are far from understood, and further clinical and experimental studies are needed to fully clarify such an effect.…”

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Cruzipain Sulfotopes-Specific Antibodies Generate Cardiac Tissue Abnormalities and Favor Trypanosoma cruzi Infection in the BALB/c Mice Model of Experimental Chagas Disease

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