The effect of laparoscopy on mast cell degranulation and mesothelium thickness in rats

Poerwosusanta, Hery; Gunadi, Gunadi; Noor, Zairin; Oktaviyanti, Ika Kustiyah; Mintaroem, Karyono; Pardjianto, Bambang; Widodo, M. Aris; Widjajanto, Edi

doi:10.1186/s12893-020-00775-y

Cited by 10 publications

(10 citation statements)

References 56 publications

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“…The control group (n = 6) did not receive pneumoperitoneum. The intervention groups of P-5 mmHg, P-8 mmHg, P-10 mmHg, and P-12 mmHg (all n = 6) were given 5, 8, 10, and 12 mmHg CO 2 pneumoperitoneum, respectively [ 6 , 12 ]. Our study strictly followed the ethical and euthanasia guidelines for animal research ( http://risetcenterfk.ulm.ac.id/euthanasia/ ).…”

Section: Methodsmentioning

confidence: 99%

“…According to the previous study [ 6 ], 60-min laparoscopy was performed in a sterile area after shaving and povidone-iodine application. Ten mg/kg BW intramuscular injections of ketamine hydrochloride (KTM-10; PT Guardian Pharmatama, No.…”

Section: Methodsmentioning

confidence: 99%

“…Mast cells are specific [ 4 ], mature in the tissues, and form 10% of the mesothelium immune cell population [ 5 ]. Laparoscopy procedures cause mast cell infiltration and degranulation [ 6 ]. The release of histamine, tryptase, and chymase due to mast cell degranulation [ 7 ] are presumed to play a role in intra-abdominal adhesion.…”

Section: Introductionmentioning

confidence: 99%

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Laparoscopic procedures impact on mast cell mediators, extracellular matrix and adhesion scoring system in rats

Poerwosusanta

Gunadi²,

Oktaviyanti

et al. 2020

Annals of Medicine and Surgery

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Background Laparoscopic procedures under certain pressure have the potential to cause intra-abdominal adhesions. However, the pathomechanism of this disorder is unknown. Release of mast cell mediators due to mast cell degranulation is thought to be the cause. Materials and methods Thirty male Sprague-Dawley rats were grouped into five groups (n = 6 per group): one control group and four intervention groups to which 60 min insufflation was performed using carbon dioxide at 5, 8, 10 and 12 mmHg. Seven days after laparoscopy, we euthanized and evaluated the levels of histamine, tryptase, and chymase of peritoneal fluid, the thickness of ECM of peritoneal tissue, and intraabdominal adhesion scoring system. Results Histamine and tryptase levels in peritoneal fluid were significantly higher at the 10- and 12 mm Hg intervention compared to control (histamine: 0.50 ± 0.35 vs. 0.41 ± 0.41 vs. 0.04 ± 0.02 ng/mL, respectively; and tryptase: 0.69 ± 0.11 vs. 0.65 ± 0.05 vs. 0.48 ± 0.02 ng/ml respectively). The ECM was significantly thicker in the intervention groups at 10- and 12-mm Hg compared to control (71.3 [66.7–85.2] vs. 48.4 [34.5–50.3] vs. 10.25 [8.7–12.1] μm, respectively). Moreover, the intra-abdominal scoring was also significantly higher in the intervention groups at 10- and 12 mm Hg compared to control (4 [0–4] vs. 4.5 [4–5], vs. 0, respectively). Conclusions Laparoscopic procedures increase the release of mast cell mediators in peritoneal fluid, the thickness of ECM and intraabdominal adhesion scoring in rats, implying that it might increase the possibility of intrabdominal adhesion in humans.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Laparoscopic procedures impact on mast cell mediators, extracellular matrix and adhesion scoring system in rats

Poerwosusanta

Gunadi²,

Oktaviyanti

et al. 2020

Annals of Medicine and Surgery

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“…Recently, similar findings were observed by Poerwosusanta et al in rats exposed to 8, 10, and 12 mmHg pneumoperitoneum. e authors found a progressive and significant increase in mastcell peritoneal infiltration and degranulation with an increase in pressure 7 days after surgery [29]. Paralleling with local acute response, gas pressure was described as a factor influencing systemic inflammation.…”

Section: Inflammationmentioning

confidence: 96%

The “Dark Side” of Pneumoperitoneum and Laparoscopy

Umano

Delehaye

Noviello

et al. 2021

Minimally Invasive Surgery

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Laparoscopic surgery has been one of the most common procedures for abdominal surgery at pediatric age during the last few decades as it has several advantages compared to laparotomy, such as shorter hospital stays, less pain, and better cosmetic results. However, it is associated with both local and systemic modifications. Recent evidence demonstrated that carbon dioxide pneumoperitoneum might be modulated in terms of pressure, duration, temperature, and humidity to mitigate and modulate these changes. The aim of this study is to review the current knowledge about animal and human models investigating pneumoperitoneum-related biological and histological impairment. In particular, pneumoperitoneum is associated with local and systemic inflammation, acidosis, oxidative stress, mesothelium lining abnormalities, and adhesion development. Animal studies reported that an increase in pressure and time and a decrease in humidity and temperature might enhance the rate of comorbidities. However, to date, few studies were conducted on humans; therefore, this research field should be further investigated to confirm in experimental models and humans how to improve laparoscopic procedures in the spirit of minimally invasive surgeries.

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“…Poerwosusanta et al investigated the role of mast cell degranulation in mesothelial injury. Mesothelial injury was carried out by performing laparoscopic surgeries in rats at different intra-abdominal inflation pressures (74). They showed that an increased intraabdominal pressure-and presumably increased stress to the mesothelium-led to an increased number of mast cells that infiltrated the mesothelium.…”

Section: Dendritic Cells and Mast Cellsmentioning

confidence: 99%

Sterile Injury Repair and Adhesion Formation at Serosal Surfaces

2021

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Most multicellular organisms have a major body cavity containing vital organs. This cavity is lined by a mucosa-like serosal surface and filled with serous fluid which suspends many immune cells. Injuries affecting the major body cavity are potentially life-threatening. Here we summarize evidence that unique damage detection and repair mechanisms have evolved to ensure immediate and swift repair of injuries at serosal surfaces. Furthermore, thousands of patients undergo surgery within the abdominal and thoracic cavities each day. While these surgeries are potentially lifesaving, some patients will suffer complications due to inappropriate scar formation when wound healing at serosal surfaces defects. These scars called adhesions cause profound challenges for health care systems and patients. Therefore, reviewing the mechanisms of wound repair at serosal surfaces is of clinical importance. Serosal surfaces will be introduced with a short embryological and microanatomical perspective followed by a discussion of the mechanisms of damage recognition and initiation of sterile inflammation at serosal surfaces. Distinct immune cells populations are free floating within the coelomic (peritoneal) cavity and contribute towards damage recognition and initiation of wound repair. We will highlight the emerging role of resident cavity GATA6+ macrophages in repairing serosal injuries and compare serosal (mesothelial) injuries with injuries to the blood vessel walls. This allows to draw some parallels such as the critical role of the mesothelium in regulating fibrin deposition and how peritoneal macrophages can aggregate in a platelet-like fashion in response to sterile injury. Then, we discuss how serosal wound healing can go wrong, causing adhesions. The current pathogenetic understanding of and potential future therapeutic avenues against adhesions are discussed.

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The effect of laparoscopy on mast cell degranulation and mesothelium thickness in rats

Cited by 10 publications

References 56 publications

Laparoscopic procedures impact on mast cell mediators, extracellular matrix and adhesion scoring system in rats

Laparoscopic procedures impact on mast cell mediators, extracellular matrix and adhesion scoring system in rats

The “Dark Side” of Pneumoperitoneum and Laparoscopy

Sterile Injury Repair and Adhesion Formation at Serosal Surfaces

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