The effect of lead ions on the energy metabolism of human erythrocytes in vitro

Baranowska-Bosiacka, Irena; Hlynczak, A.J.

doi:10.1016/s1532-0456(03)00008-5

Cited by 28 publications

(16 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because no DNA synthesis occurs, only ribonucleotides are present. The adenine ribonucleotide pool, which in metabolically normal erythrocytes mainly consists of adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP), is much larger than that of other nucleotides, such as the guanine ribonucleotides (GTP, GDP, and GMP) [1]. Generally, ADP and AMP concentrations are only 12.5-20% and 1-2%, respectively, of the ATP concentrations [2].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of adenosine triphosphate and its metabolites in human whole blood by RP-HPLC and UV-detection

Coolen

Arts

Swennen

et al. 2008

Journal of Chromatography B

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of adenosine triphosphate and its metabolites in human whole blood by RP-HPLC and UV-detection

Coolen

Arts

Swennen

et al. 2008

Journal of Chromatography B

View full text Add to dashboard Cite

“…[51] It may as well be suggested that these observed alterations in the cellular integrity are due to excess Pb stored in the interneuronal spaces that inhibits oxygen utilization, thus reducing the production of the required level of ATP through the electron transport chain and modifying the morphology of the neuron to compensate for the available amount of energy present. [52,53] Withdrawing the rats from further exposure to Pb did not bring any form of significant improvement in the cytoarchitecture of the mPFC compared with the control group (Figures 4a versus 4c; 5a versus 5c; 6a versus 6c). This is in agreement with earlier studies that suggested that cellular improvement from Pb exposure was never complete.…”

Section: Discussionmentioning

confidence: 92%

Lead induces inflammation and neurodegenerative changes in the rat medial prefrontal cortex

Adekomi¹,

Stephen²,

Adekilekun³

et al. 2017

Anatomy

View full text Add to dashboard Cite

Objectives: Lead (Pb) is a neurotoxicant heavy metal ubiquitously present in the eco-system. The precise mechanism by which Pb confers its deleterious effects on the cellular profile of the central nervous system remains unknown. The aim of this study was to investigate the effect of Pb on the medial prefrontal cortex (mPFC) using histological, immunohistological and morphological techniques.Methods: Thirthy juvenile male Wistar rats were used in this study. The rats were randomly assigned into three groups. Group A served as the control group, Group B received 5 mg/kg Pb-nitrate (PbNO3) orally for 21 days, and Group C received 5 mg/kg PbNO3 and left for an additional 21 days to recover.Results: There was a significant decrease in the number of normal neurons in the mPFC of the PbNO3-treated rats. The number of degenerating neurons significantly increased in the PbNO3-treated groups compared with the control group. A marked increase was observed in the number of astrocytic cell count in the PbNO3-treated groups compared with the control. The neuronal cells in the cytoarchitectural profile of the mPFC of the rats receiving PbNO3 showed marked neurodegenerative modification with features of distorted morphology, swollen and vacuolized cytoplasm, and features of either pyknotic or karyorrhectic nuclei. The cytoarchitecture of the mPFC of the rats in the control group preserved the normal histological outline suggestive of a normal and functional mPFC. Conclusion:Exposure to Pb ingestion can result in significant inflammatory responses in the cytoarchitectural profile of the mPFC. Furthermore, 21 days of cessation of exposure to PbNO3 did not halt or reverse the deleterious effects of Pb on the mPFC of the rats, suggesting that Pb persists in the central nervous system of the rats.

show abstract

“…Lead may inhibit the body's ability to make Hb by interfering with several enzymatic steps in the heme pathway. Specifically lead decreases heme biosynthesis by inhibition of ë-aminolevulinic acid dehydratase and ferrochelatase activity [33,34] By pollution or feeding lead induced toxicity in the body affects metabolism through derangement of antioxidant enzymes which may upset the metabolism of lipids such as cholesterol, lipoproteins and triglycerides. Here inhibition of anti oxidant bio molecules such as GSH and anti oxidant enzymes adversely affected the repair of tissues due to lead toxicity.…”

Section: Discussionmentioning

confidence: 99%

Prophylactic Effects of Garlic Oil and Onion Oil Fractions as Compared to Vitamin E on Rats Orally Fed with Lead Acetate Solution

2016

View full text Add to dashboard Cite

Heavy metal pollution is a global public health challenge due to its stable and persistent environmental contamination. Of these lead is considered to be one of the most common ubiquitous and industrial pollutants and at low concentration it exerts extensive damages to the tissues. Daily feeding of lead acetate solution (Dose: 10 mg/ kg/day) to normal rats for a month adversely altered the parameters of blood, serum and tissues, viz; RBC, WBC, Hb, ë-ALAD (Delta amino levulinic acid dehydratase), Pb content, lipids, oxidized lipids (TBARS), vitamins C and E and GSH levels and activities of AST, ALT and antioxidant enzymes viz; catalase, GR, Gpx and SOD. In order to study whether antioxidants have any effect to counteract the toxicity of lead we have selected comparatively better active allium fractions for the study viz: polar fraction of garlic (PFG) and polar fraction of onion (PFO). On feeding of these active fractions of garlic and onion oils i.e. their polar fractions and vitamin E (Dose 100 mg/kg/day) separately for a month along with or without lead acetate to rats each nutraceutical and vitamin E counteracted the adverse effects of Pb significantly (p B 0.05). Their effects are in the order of PFG [ PFO [ Vitamin E. All these results point out that garlic and onion oils contain natural disulfoxide compounds which act as antioxidant and anti toxic to lead compounds. Their comparative differences in action may be due to the presence and position of double bonds and disulfide oxide bonds in their molecules. i.e., in PFG the allyl disulfide oxide group is present and in PFO saturated methyl and propyl groups and unsaturated propenyl group are present in place of allyl groups. The former group confers a better antioxidant activity on PFG, while the latter groups confer a lesser activity on PFO.

show abstract

The effect of lead ions on the energy metabolism of human erythrocytes in vitro

Cited by 28 publications

References 34 publications

Simultaneous determination of adenosine triphosphate and its metabolites in human whole blood by RP-HPLC and UV-detection

Simultaneous determination of adenosine triphosphate and its metabolites in human whole blood by RP-HPLC and UV-detection

Lead induces inflammation and neurodegenerative changes in the rat medial prefrontal cortex

Prophylactic Effects of Garlic Oil and Onion Oil Fractions as Compared to Vitamin E on Rats Orally Fed with Lead Acetate Solution

Contact Info

Product

Resources

About