The Effect of Lemongrass Leaves and Stalks Extracts using Methanol as The Eco-friendly Larvicides on Fourth Instar Aedes aegypti Larvae

Zanaria, Tjut Mariam; Oktiviyari, Ade; Harahap, Ahmad Yamin

doi:10.3889/oamjms.2021.6727

Cited by 2 publications

(1 citation statement)

References 16 publications

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“…The Cymbopogon genus plant has a wide range of applications, including common tea, medicinal supplement, insect repellant, pesticide, flu control, antiinflammatory, and analgesic properties [6], [7], [8]. Conventionally, Cymbopogon winterianus was used to make aromatic tea, as a vermifuge, diuretic, and antispasmodic [9], and to treat fever, intestinal parasites, digestive, and menstrual issues.…”

Section: Introductionmentioning

confidence: 99%

Essential Oil Constituents and Pharmacognostic Evaluation of Java Citronella (Cymbopogon winterianus) stem from Bandung, West Java, Indonesia

Haerussana

Chairunnisa

2022

Open Access Maced J Med Sci

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BACKGROUND: Cymbopogon winterianus essential oil contains citral-derived chemicals with a variety of pharmacological effects, although there has been minimal research on pharmacognostic, phytochemical, and biological aspects. AIM: This research aims to evaluate the pharmacognostic and chemical components of C. winterianus stem essential oil. METHODS: The pharmacognostic studies were carried out in terms of macroscopic, microscopic, water content, water extractive values, ethanol extractive values, and essential oil yield. The oil was extracted by ethanol steam distillation method, the oil composition was analyzed by GC-MS. RESULTS: Cymbopogon winterianus stems are 15-35 cm long and 0.5-2 cm broad, with a rough, stiff, and thin texture, with prominent fibers on the top and bottom surfaces. It has a bitter and slightly spicy taste with a distinctive lemony aroma and was greenish yellow in hue with a blend of purplish-red colors. The upper and lower epidermis was examined under a microscope, which revealed sclerenchyma fibers, trichomes, parenchyma, calcium oxalate (rosette), cortex, stem pith, oil cells, stomata (Gramineae type), trachea (ladder and spiral thickening), and collenchyma. The water content was 7.16±0.72%, the water-soluble extractive value was 12.152±0.003%, the ethanol-soluble extractive value was 12.290 ±0.76%, and the essential oil content was 6.46±0.50%. Essential oil constituents were 6-octen-1-ol, 3,7-dimethyl-, formate; trifluoroacetyl-lavandulol; n-hexadecanoic acid; 9,12-octadecadienoic acid (Z,Z)-; oxacyclotetradecane-2,11-dione, 13-methyl-; palmitic acid vinyl ester; glycidyl palmitate; 9,17-Octadecadienal, (Z)-; glycidyl palmitoleate; 1,4-bis(trimethylsilyl)benzene; and cyclotrisiloxane, hexamethyl-. CONCLUSION: The essential oil constituents and preliminary pharmacognostic evaluation of Cymbopogon winterianus stem can provide useful data for further phytochemical analysis, quality control, and standardization of Cymbopogon winterianus. Citronellyl formate detected as citral derivatives as the main compound in the ethanol steam distillation method.

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Section: Introductionmentioning

confidence: 99%

Essential Oil Constituents and Pharmacognostic Evaluation of Java Citronella (Cymbopogon winterianus) stem from Bandung, West Java, Indonesia

Haerussana

Chairunnisa

2022

Open Access Maced J Med Sci

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Larvicidal Potency of Ashes of Two Insecticidal Plants against the Activities of Anopheles coluzzii and Culex quinquefasciatus Mosquitoes

Ojianwuna,

Enwemiwe,

Esiwo

et al. 2024

TOECOLJ

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Introduction Insecticidal plants are widely studied materials that have intense applications in various fields of vector, pest, and disease control. They are among the recommended strategies to tackle the already established resistance in mosquitoes causing prevailing diseases in the world, especially in Africa. Aim and Objective The study aimed to assess the biological potency of ashes of scent leave (Ocimum gratissimum) and lemon grass (Cymbopogon citratus) against the larvae of Anopheles and Culex mosquitoes. Methods Larvae of mosquitoes were sourced, and plant ashes were prepared and exposed to 20 larvae of both mosquitoes in 1g, 2.5g, 5g, 10g, and 15g concentrations. Treatment concentrations were formulated by mixing ashes in 100ml of water in triplicate. Mortality, acute toxicity, and sub-chronic toxicity data were obtained. Results Mosquito larval mortality increased with time at all concentrations of test plants, and sub-chronic toxicity showed complete mortality in all treatments. Acute toxicity of Culex larvae was highest in 15g of lemon grass and complete mortality was recorded after 30 minutes of exposure. There was no acute toxicity recorded with scent leave exposure. The Lethal Dose (LD50) for Anopheles mosquitoes recorded with scent leave ashes was 0.319g (y=1.928x+0.96; R2=0.221, p= 0.407), and for Culex mosquitoes, it was recorded to be 0.424g with lemon grass exposure (y=1.86x+0.69; R2=0.221, p= 0.240). Remarkably, lemon grass at a concentration of 1.250g and 3.247g caused 95% toxicity in Anopheles (y=15.85x-0.25; R2=0.633, p> 0.05) and Culex (y=2.918x-0.18; R2=0.388, p= 0.254) mosquitoes, respectively. LT50 of mosquitoes was between 21.3 minutes and 1451.4 minutes, whereas LT95 was between 37.1 minutes and 1740.4 minutes, respectively. No adult mosquito emergence was recorded. Conclusion Ashes of both plants, especially lemon grass, could be considered better materials for local treatment of the mosquito breeding sites.

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The Effect of Lemongrass Leaves and Stalks Extracts using Methanol as The Eco-friendly Larvicides on Fourth Instar Aedes aegypti Larvae

Cited by 2 publications

References 16 publications

Essential Oil Constituents and Pharmacognostic Evaluation of Java Citronella (Cymbopogon winterianus) stem from Bandung, West Java, Indonesia

Essential Oil Constituents and Pharmacognostic Evaluation of Java Citronella (Cymbopogon winterianus) stem from Bandung, West Java, Indonesia

Larvicidal Potency of Ashes of Two Insecticidal Plants against the Activities of Anopheles coluzzii and Culex quinquefasciatus Mosquitoes

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