The effect of leptin and resveratrol on JAK/STAT pathways and Sirt-1 gene expression in the renal tissue of ischemia/reperfusion induced rats

Abstract

OBJECTIVE: Our study aimed to investigate the possible modifying effects of leptin and combined use of resveratrol on rat renal I/R injury and their relationship on signal pathways and apoptosis-related mechanisms. BACKGROUND: Renal ischemia-reperfusion (I/R) injury is an important cause of acute renal failure. METHODS: Male Sprague Dawley rats were divided into 5 groups: Control, I/R, I/R+leptin, I/R+resveratrol and I/R+leptin+resveratrol. Leptin (10 μg/kg BW) was administered (i.p.) 30 min prior to I/R. Resveratrol was administered by gavage at 20 mg/kg BW per d for 12 d prior to I/R. The left renal artery was exposed to 1 h of ischemia and 1 h of reperfusion. RESULTS: Resveratrol treatment alone increased TNF-α, TNF-α R1, NF-κB, SIRT-1, STAT1 and STAT3 mRNA levels and decreased caspase 3 protein levels. Leptin treatment alone signifi cantly decreased the caspase 3 protein levels. The combined use of resveratrol and leptin signifi cantly increased STAT3, and caspase 3 mRNA levels, and decreased the caspase 3 protein levels. Apoptosis was signifi cantly decreased especially in the leptin and leptin+resveratrol groups. CONCLUSION: The present study suggest that a combined use of resveratrol and leptin has preventive and regulatory effects on renal I/R injury; the mechanism involves decreasing apoptosis, likely by altering the JAK/ STAT pathway and SIRT1 expression (Fig. 8, Ref. 24). Text in PDF www.elis.sk.

Expression Levels of Predominant Adipokines and Activations of STAT3, STAT6 in an Experimental Mice Model of Obese Asthma

“…Furthermore, previous studies have demonstrated a role of leptin on regulating the JAK/STAT signaling pathway. 17,18 Therefore, together with our data, the function of leptin on the pulmonary inflammation may be partly through activating the STAT3 signaling pathway.…”

“…Furthermore, a few researches have demonstrated that leptin positively increased the STAT3 activation in inflammatory diseases including chronic inflammatory lung diseases. 17,18 Therefore, this study was designed to detect the expression levels of leptin, adiponectin, STAT3 and STAT6 mRNA and proteins in lungs and bronchoalveolar lavage fluid (BALF) as well as the activations of STAT3 and STAT6 protein in the lung tissues and evaluate the differences between the mRNA and protein expressions in obese and lean mice in experimental asthmatic model.…”

Polyphenols in Liubao Tea Can Prevent CCl4-Induced Hepatic Damage in Mice through Its Antioxidant Capacities

“…Binding of TNF-α and liver cell membrane TNF-α receptor 1 (TNF-αR1) can induce intracellular double-stranded DNA to fragment, thereby resulting in stem cell apoptosis. In addition, TNF-α triggers inflammatory responses by activating NF-κB, which exacerbates liver injury [ 37 ]. IFN-γ is a proinflammatory cytokine that increases the sensitivity of hepatocytes to TNF-α, rendering hepatocytes to further damage [ 38 ].…”

HO-1 Overexpressed Mesenchymal Stem Cells Ameliorate Sepsis-Associated Acute Kidney Injury by Activating JAK/stat3 Pathway

“…JAK/STAT pathway play roles in acute kidney injury, 4,23 and studies indicated that JAK/STAT pathway is associated with the MSC-mediated tissue repair. 15 Herein we hypothesize that engineering MSCs with overexpressed HO-1 can improve treatment efficacy for AKI.…”