The effect of Lidocaine on the viability of cultivated mature human cartilage cells: an in vitro study

Jacobs, Tom; Vansintjan, Pieter S.; Roels, Nathalie; Herregods, Sofie S.; Verbruggen, Gust; Herregods, Luc; Almqvist, Karl

doi:10.1007/s00167-011-1420-5

Cited by 56 publications

(61 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…That is particularly evident after cells exposure to 1% L or L+A: in the first case viability was statistically reduced with respect to controls at all observation times, whereas in the second case the viability was significantly reduced only 1 h after exposure. Similar results were obtained by Jacobs et al, (2011) following the exposure of human chondrocytes to 1% L and 1% L + A. These authors speculated that adrenaline may exert a protective effect on chondrocytes but the data presented in the literature are equivocal.…”

Section: Discussionsupporting

confidence: 68%

Intra-articular administration of lidocaine plus adrenaline in dogs: Pharmacokinetic profile and evaluation of toxicity in vivo and in vitro

Salvo

Chiaradia

Rocca

et al. 2016

The Veterinary Journal

View full text Add to dashboard Cite

Highlights The potential for cardio-, neuro-and chondro-toxicity of intra-articular administration of lidocaine plus adrenaline was evaluated in dogs undergoing arthroscopy  The treatment was not found to cause adverse effects on the cardiovascular system.  The plasma concentrations of lidocaine did not reach neurotoxic concentrations.  Toxic effects of lidocaine on chondrocytes appeared to be reduced by adrenaline. AbstractThe aim of this study was to evaluate the safety of intra-articular (

show abstract

Section: Discussionsupporting

confidence: 68%

Intra-articular administration of lidocaine plus adrenaline in dogs: Pharmacokinetic profile and evaluation of toxicity in vivo and in vitro

Salvo

Chiaradia

Rocca

et al. 2016

The Veterinary Journal

View full text Add to dashboard Cite

show abstract

“…Bupivacaine 0.25% reduced cell viability to 72% in the monolayer cultures but buffering the pH resulted in worsening of cell viability to 22%. Jacobs et al 30 suggested that adrenaline may even have a protective effect on articular cartilage. They harvested articular cartilage from the knees of four human donors and three patients undergoing TKA and cultured the chondrocytes in alginate bead cultures.…”

Section: S204mentioning

confidence: 99%

Articular cartilage and local anaesthetic: A systematic review of the current literature

Gulihar

Robati

Twaij

et al. 2015

Journal of Orthopaedics

View full text Add to dashboard Cite

“…This result is different from the previous report molecularly that is devastating results on chondrocytes after lidocaine treatment. Exposing the chondrocytes to a 50/50 mixture of culture medium and local anesthetics substantially decreased cytotoxicity but still showed high toxicity when compared with the saline group (90% dead cells for lidocaine 2%, P = 0.047) [15]. In this study, the high-toxicity was not presented, considering a 50/50 mixture of culture medium and 2% lidocaine was used.…”

Section: Discussionmentioning

confidence: 87%

The effects of lidocaine and procaine on microRNA expression of adipocyte-derived adult stem cells

Sung

Lee

et al. 2012

Korean J Anesthesiol

View full text Add to dashboard Cite

BackgroundThe microRNA (miRNA) pathway has emerged as one of the biologic pathways implicated in stem cell regulation. miRNA is a noncoding, single-stranded RNA consisting of 20-25 nucleotides that inhibits the protein production at the step of translation. The molecular effects of lidocaine and procaine on adipose stem cells were investigated by examining RNA expression array.MethodsAdipose stem cells were isolated from a prior abdominal liposuction procedure. The human adipose stem cells were cultured and then added to a mixture of 1 ml of culture medium plus 1 ml of 2% lidocaine or 2% procaine for the duration of 30 minutes. The expression levels of miRNAs were estimated by using peptide nucleic acid (PNA)-miRNA array analysis throughout the denaturation and hybridization processes after the isolation of miRNA. The miRNAs detected by microarray that either decreased by half fold or increased by 1.5 fold from the control level were interpreted as significant.ResultsAccording to microarray analysis there were 61 miRNAs in total, and no miRNA had decreased expression levels. The stem cells treatment with lidocaine showed 4 alteration of expression with miR-9a* (1.53 fold), miR-29a (1.64 fold), miR-296-5p (1.64 fold) and miR-373 (1.94 fold). The stem cells treated with procaine showed 32 miRNAs that were significantly up-regulated with a range of 1.5 to 2.06 fold. They were stem cell differentiation-related miRNAs, apoptosis and cell cycle-associated miRNAs, immunity-associated miRNAs and hormonal response-related miRNAs.ConclusionsLidocaine and procaine affect the miRNA expression on adipose stem cells and the effect of procaine is more marked than that of lidocaine.

show abstract

The effect of Lidocaine on the viability of cultivated mature human cartilage cells: an in vitro study

Cited by 56 publications

References 32 publications

Intra-articular administration of lidocaine plus adrenaline in dogs: Pharmacokinetic profile and evaluation of toxicity in vivo and in vitro

Intra-articular administration of lidocaine plus adrenaline in dogs: Pharmacokinetic profile and evaluation of toxicity in vivo and in vitro

Articular cartilage and local anaesthetic: A systematic review of the current literature

The effects of lidocaine and procaine on microRNA expression of adipocyte-derived adult stem cells

Contact Info

Product

Resources

About