The effect of light intensity on the production of oat (<i>Avena sativa</i>L.) doubled haploids through oat × maize crosses

Skrzypek, Edyta; Warchoł, Marzena; Czyczyło-Mysza, Ilona; Marcińska, Izabela; Nowakowska, Agata; Dziurka, Kinga; Juzoń, Katarzyna; Noga, Angelika

doi:10.1556/0806.44.2016.007

Cited by 13 publications

(17 citation statements)

References 18 publications

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“…Pollinated flowers were treated with one drop of 2,4-dichorophenoxyacetic acid (2,4-D) solution (50 mg dm −3 ) on the next day. The protocol of the wide crossing method for the production of oat doubled haploids (DH) has been previously described in details by Skrzypek et al (2016), Warchoł et al (2016), and Nowakowska et al (2015). Enlarged oat ovaries were collected 21 d after pollination with maize.…”

Section: Methodsmentioning

confidence: 99%

Endogenous phytohormone profile during oat (Avena sativa L.) haploid embryo development

Dziurka

Warchoł

et al. 2019

In Vitro Cell.Dev.Biol.-Plant

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The development of embryos requires interaction of many endogenous hormones. The aim of the study was to determine which endogenous phytohormones are involved in the process of oat (Avena sativa L.) haploidization. Oat haploids were obtained by wide crossing with Zea mays L. The hormonal profiles of the ovaries with (OE) and without developed embryo (OWE) were compared. Phytohormone contents were measured by UHPLC coupled with mass spectrometer. The total content of indole-3acetic acid (IAA), trans-zeatin (tZ), and kinetin (KN) in OE was significantly higher than in OWE. 4-Chloroindole-3-acetic acid was detected only in OWE. There were no differences between OE and OWE in the content of gibberellins (GA 1 , GA 3 , GA 4 , GA 6 , GA 7 ) and stress hormones (abscisic, salicylic, jasmonic acids). Content of endogenous KN was highly negatively correlated with the percentage of haploid embryos, germinated haploid embryos, haploid plants on MS (in vitro), haploid plants in soil (ex vitro), and doubled haploid lines. The tZ content negatively correlated with the frequency of haploid embryo formation, germination, and haploid plants obtained in vitro, as opposed to GA 1 , which correlated positively. A positive correlation was found between IAA and tZ in OE, whereas in OWE it was a negative correlation. The profiles of phytohormones in OE and OWE were determined; however, their mode of action needs to be clarified.

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Section: Methodsmentioning

confidence: 99%

Endogenous phytohormone profile during oat (Avena sativa L.) haploid embryo development

Dziurka

Warchoł

et al. 2019

In Vitro Cell.Dev.Biol.-Plant

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“…ex A. Camus ) are examples of crops where DH technologies are less advanced, but still hundreds of DHs can be obtained (Tuvesson et al 2007). Wide crossing with maize is the main and most-effective method for producing oat DH lines (Rines 2003; Marcińska et al 2013; Nowakowska et al 2015; Warchoł et al 2016; Skrzypek et al 2016). …”

Section: Introductionmentioning

confidence: 99%

Conversion of oat (Avena sativa L.) haploid embryos into plants in relation to embryo developmental stage and regeneration media

Noga

Skrzypek

Warchoł

et al. 2016

In Vitro Cell.Dev.Biol.-Plant

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Obtaining oat DH lines is only effective via wide crossing with maize. Seven hundred haploid embryos from 21 single F1 progeny obtained from wide crosses with maize were isolated, divided into four groups according to their size (<0.5 mm, 0.5–0.9 mm, 1.0–1.4 mm, and ≥1.5 mm), and transferred into 190–2 regeneration medium with different growth regulators: 0.5 mg L−1 kinetin (KIN) and 0.5 mg L−1 1-naphthaleneacetic acid (NAA); 1 mg L−1 zeatin (ZEA) and 0.5 mg L−1 NAA; or 1 mg L−1 dicamba (DIC), 1 mg L−1 picloram (PIC), and 0.5 mg L−1 kinetin (KIN). Among all isolated embryos, approximately 46.1% were between 1.0–1.4 mm, while the smallest group of embryos (7.1%) were those <0.5 mm. The ability of haploid embryos to germinate varied depending on oat genotypes and the size of embryos. Haploid embryos <0.5 mm were globular and did not germinate, whereas embryos ≥1.5 mm had clearly visible coleoptiles, radicles, and scutella, and were able to germinate. Germination of oat haploid embryos varied depending on growth regulators in the regeneration medium. Most haploid embryos germinated on medium with 0.5 mg L−1 NAA and 0.5 mg L−1 KIN, while the fewest germinated on medium with 1 mg L−1 DIC, 1 mg L−1 PIC, and 0.5 mg L−1 KIN. One hundred thirty germinated haploid embryos converted into haploid plants. Fifty oat DH lines were obtained after colchicine treatment.Electronic supplementary materialThe online version of this article (doi:10.1007/s11627-016-9788-z) contains supplementary material, which is available to authorized users.

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“…saccharata): MPC4, Dobosz and Wania obtained from Małopolska Plant Breeding Ltd. HBP Polanowice were used as pollen donors. The mixture of maize pollen was chosen according to Skrzypek et al (2016). Oat and maize were grown in the glasshouse under natural (solar) light intensity (photosynthetic active radiation (PAR) was 800 µmol m − 2 s − 1 ) and 16-h light/8-h dark.…”

Section: Plant Materialsmentioning

confidence: 99%

“…In Avena sativa L., DH lines were produced by in vitro microspore or anther culture (Kiviharju et al 2005;Tanhuanpää et al 2008;Ponitka and Ślusarkiewicz-Jarzina 2009;Sidhu and Davies 2009;Ferrie et al 2014), and by distant crosses with maize (Zea mays L.) (Sidhu et al 2006;Nowakowska et al 2015;Warchoł et al 2016;Skrzypek et al 2016).…”

Section: Introductionmentioning

confidence: 99%

“…The most frequently used sucrose concentrations in the culture of oat haploid embryos were 2, 3 and 7% (Kynast and Riera-Lizarazu 2011;Ishii et al 2013). In addition, maltose supplementation (6 and 9%) was used for oat haploid embryos instead of sucrose in the germination media (Marcińska et al 2013;Nowakowska et al 2015;Noga et al 2016;Skrzypek et al 2016;Warchoł et al 2016). Lulsdorf et al (2014) reported that the culture of monocotyledonous plants is more simple than the dicocotyledonous plants culture, which requires a multistage procedure for embryos culture, as well as shoot and root development.…”

Section: Introductionmentioning

confidence: 99%

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The effect of genotype, media composition, pH and sugar concentrations on oat (Avena sativa L.) doubled haploid production through oat × maize crosses

et al. 2018

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Doubled haploid (DH) technology in oat has not reached the same stage as in other cereals leading to its application in plant breeding. The objective of this investigation was to increase the effectiveness of Avena sativa L. haploid embryo germination obtained by the distant crosses with maize. Developed embryos (obtained from 22 genotypes) were transferred on five germination media: MS (Murashige and Skoog, Physiol Plant 15:473-497, 1962) with 3% sucrose, pH 5.8 (control medium), and 190-2 supplemented with 6 and 9% maltose. The pH of 190-2 was adjusted to 5.5 and 6.0. Of all tested genotypes, 591 haploid embryos were obtained, almost half of them (279) germinated. The rate of haploid embryo germination induced on 190-2 was 6.92%, while in MS it was 3.25%. The sugar and its concentration significantly affected the germination of haploid embryos. The highest percentage of haploid embryo germination (9.11%) and DH lines production (1.64%) was achieved on 190-2 with 9% maltose and pH 6.0. All DH lines are incorporated to breeding programs for the development of new cultivars.

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The effect of light intensity on the production of oat (Avena sativaL.) doubled haploids through oat × maize crosses

Cited by 13 publications

References 18 publications

Endogenous phytohormone profile during oat (Avena sativa L.) haploid embryo development

Endogenous phytohormone profile during oat (Avena sativa L.) haploid embryo development

Conversion of oat (Avena sativa L.) haploid embryos into plants in relation to embryo developmental stage and regeneration media

The effect of genotype, media composition, pH and sugar concentrations on oat (Avena sativa L.) doubled haploid production through oat × maize crosses

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