The effect of lipoic acid on acrylamide-induced neuropathy in rats with reference to biochemical, hematological, and behavioral alterations

Abstract

Context: Acrylamide (ACR) is a well-known neurotoxicant and carcinogenic agent which poses a greater risk for human and animal health. Objective: The present study evaluates the beneficial effects of a-lipoic acid (LA) on ACR-induced neuropathy. Materials and methods: A total of 40 male rats were divided into four groups: a placebo group; LA-treated group, administered orally 1% (w/w) LA mixed with diet; ACR-treated group, given 0.05% (w/v) ACR dissolved in drinking water; and LA + ACR-treated group, given LA 1% 7 d before and along with ACR 0.05% for 21 d. After 28 d, blood samples were collected, the rats were decapitated, and the tissues were excised for the measurement of brain biomarkers, antioxidant status, and hematological analysis. Also, the gait score of rats was evaluated. Results: ACR-exposed rats exhibited abnormal gait deficits with significant (p50.05) decline in acetylcholine esterase (AChE) and creatine kinase in serum and brain tissues, respectively. However, the lactate dehydrogenase activity was increased in serum by 123%, although it decreased in brain tissues by À74%. ACR significantly (p50.05) increased the malondialdehyde level by 273% with subsequent depletion of glutathione S-transferase (GST), glutathione peroxidase (GPx), and glutathione reductase (GR) activities and reduced the glutathione (GSH) level in brain tissue. Interestingly, LA significantly (p50.05) improved brain enzymatic biomarkers, attenuated lipid peroxidation (LPO), and increased antioxidant activities compared with the ACR-treated group. Discussion and conclusion: These results suggested that LA may have a role in the management of ACR-induced oxidative stress in brain tissues through its antioxidant activity, attenuation of LPO, and improvement of brain biomarkers.

Protective Efficacy of Vitamin F against Acrylamide Induced Toxicity: Studies on Oxidative Stress Biomarkers

“…Our study found that the level of thiobarbituric acid reactive products had increased significantly in liver, kidney and brain after administrated with AA due to the failure to detoxify the AA. Similar results noted by many researchers [8,14,32].…”

Protective Role of Kaempferol Against Acrylamide Intoxication

“…Similar results noted by indicates many researchers. [23][24][25] A induced toxicity may be due to depletion in antioxidant enzymes as observed in present study which indicates loss of free radical scavenging activity. A exposure significantly inhibited the antioxidant enzymatic activities CAT and SOD levels in liver, kidney and brain.…”

Protective Effect of Selenium Nanoparticles against Acrylamide-Induced Hepatotoxicity in Albino Rats

“…ACR is well-known neurotoxicant that induced several biochemical, hematological, and behavioral alterations experimental animals (Lebda et al, 2015). Several efforts have been made to reduce the negative effects of acrylamide in foods by reducing acrylamide formation during food processing and/or by using certain food additives (Krishnakumar and Visvanathan, 2014;Lebda et al, 2015). Therefore, the present study was designed to investigate the ameliorative effect of chitosanstabilized SeNPs on hepatotoxicity of acrylamide in the experimental albino rats.…”

“…Several studies have indicated the intensive presence of acrylamide (ACR) in foods exposed to high temperatures, particularly starchy foods such as potatoes, cereals and bakery products (Riboldi et al, 2014;Surdyk et al, 2004;Tareke et al, 2002). ACR is well-known neurotoxicant that induced several biochemical, hematological, and behavioral alterations experimental animals (Lebda et al, 2015). Several efforts have been made to reduce the negative effects of acrylamide in foods by reducing acrylamide formation during food processing and/or by using certain food additives (Krishnakumar and Visvanathan, 2014;Lebda et al, 2015).…”