The effect of LLLT at 5 mW on corneal alkali burn rat model

Abstract

Purpose: This study aimed to investigate the effect of 5mW low-level light therapy (LLLT) on corneal alkali burn rat model, as no prior research has examined this specific treatment parameter. Method: Eighteen male SD rats were randomly assigned to three groups: control, 5-minute LLLT, and 10-minute LLLT. Corneal alkali burns were induced using 1N NaOH, and LLLT was performed daily with an 830-nm LED device. Ocular signs and immunohistochemistry analysis were conducted. Result: LLLT-treated groups exhibited faster wound healing at 12 hours but had higher cataract incidence. Neovascularization and corneal opacity scores were significantly higher in LLLT-treated groups. Immunohistochemical analysis showed increased IL-1β and IL-6 levels in cornea and retina. Discussion: LLLT accelerated wound healing but increased cataract formation, corneal opacity, neovascularization, and inflammatory response in ocular tissues. LLLT may have therapeutic potential for corneal alkali burns, but further studies are necessary to optimize treatment parameters and mitigate adverse effects.