The effect of low-intensity pulsed ultrasound on wound healing using scratch assay in epithelial cells

Iwanabe, Yujiro; Masaki, Chihiro; Tamura, Akiko; Tsuka, Shintaro; Mukaibo, Taro; Kondo, Yusuke; Hosokawa, Ryuji

doi:10.1016/j.jpor.2016.03.002

Cited by 27 publications

(18 citation statements)

References 38 publications

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“…We analyzed the expression of these two markers and found that an increase of E‐Cadherin and a decrease of Vimentin are induced by LIPUS treatment, thus suggesting their ability to halt the EMT process or, vice versa, to favor the mesenchymal to epithelial transition (MET; 1,000 kHz, p ≤ .01; 4500 kHz, p ≤ .01 for E‐Cadherin in HT29; 1000 kHz, p ≤ .05; 4,500 kHz, p ≤ .05 for E‐Cadherin in Caco2; 1,000 kHz, p ≤ .01; 4,500 kHz, p ≤ .01 for Vimentin in HT29; 1000 kHz, p ≤ .05; 4,500 kHz, p ≤ .01 for Vimentin in Caco2 (Figure 5a,b). LIPUS has been reported to shorten the time required for wound healing in gingival epithelial cells (Iwanabe et al, 2016). Here, we tested the effects of LIPUS on wound healing closure in both colorectal cancer cell lines.…”

Section: Resultsmentioning

confidence: 99%

“…We analyzed the expression of these two markers and found that an increase of E-Cadherin and a decrease of Vimentin are induced by LIPUS treatment, thus suggesting their ability to halt the EMT process or, vice versa, to favor the mesenchymal to epithelial (Figure 5a,b). LIPUS has been reported to shorten the time required for wound healing in gingival epithelial cells (Iwanabe et al, 2016).…”

Section: Us Alters Cell Viability and Clonogenicity Of Colon Cancermentioning

confidence: 99%

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Low‐intensity pulsed ultrasound affects growth, differentiation, migration, and epithelial‐to‐mesenchymal transition of colorectal cancer cells

Lucchetti

Perelli

Colella

et al. 2020

Journal Cellular Physiology

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Ultrasound (US) offers potentially important opportunities from a therapeutic point of view. Thus, the study of the biological effects of US on cancer cells is important to understand the consequences of these changes on the malignant phenotype. This study aimed to investigate the effects of low‐intensity ultrasound (LIPUS) on the phenotype of colorectal cancer cell lines. Cell proliferation was evaluated by viability test and by evaluation of pERK expression, while cell motility using the scratch test. Cell differentiation was evaluated assessing alkaline phosphatase activity. Epithelial mesenchymal transition was assessed by analyzing the expression of Vimentin and E‐Cadherin. Release and uptake of extracellular vesicles (EVs) were evaluated by flow cytometry. LIPUS effects on the organization of cytoskeleton were analyzed by confocal microscopy and by evaluation of Rho GTPase expression. No alterations in vitality and clonogenicity were observed when the intermediate (0.4 MPa) and the lowest (0.035 MPa) acoustic intensities were administered while the treatment with high intensity (1 MPa) induced a reduction of both cell viability and clonogenicity in both cell lines in a frequency‐dependent manner. LIPUS promoted the differentiation of colon cancer cells, affected epithelial‐to‐mesenchymal transition, promoted the closure of a wound as well as increased the release of EVs compared with untreated cells. LIPUS‐induced increase in cell motility was likely due to a Rho GTPase‐dependent mechanism. Overall, the results obtained warrant further studies on the potential combined effect of LIPUS with differentiating agents and on their potential use in a clinical setting.

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Section: Resultsmentioning

confidence: 99%

Section: Us Alters Cell Viability and Clonogenicity Of Colon Cancermentioning

confidence: 99%

Low‐intensity pulsed ultrasound affects growth, differentiation, migration, and epithelial‐to‐mesenchymal transition of colorectal cancer cells

Lucchetti

Perelli

Colella

et al. 2020

Journal Cellular Physiology

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“…The LIPUS generating system with six individual ultrasound transducers was previously described3738. The culture plate was placed above these transducers with a thin layer of coupling gel3940. The wells vertically above the transducers were used for experiments.…”

Section: Methodsmentioning

confidence: 99%

Low-intensity pulsed ultrasound (LIPUS) prevents periprosthetic inflammatory loosening through FBXL2-TRAF6 ubiquitination pathway

Zhao

Zhao²,

Shi

et al. 2017

Sci Rep

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Previous studies have shown that Low intensity pulsed ultrasound(LIPUS) prevents polyethylene-debris-induced periprosthetic loosening in vivo, but the details of the mechanism by which it does so remain unclear. In this article, we used polyethylene debris induced RAW 264.7 cells as the in vitro model, and tested the effect of LIPUS on this model. Changes in the level of inflammatory cytokines, cell proliferation, and apoptosis were assessed. Gene overexpression and siRNA technique were applied, and the levels of expression of FBXL2, TRAF6, ERK, and related inflammatory cytokines were also measured. Results indicated that FBXL2-mediated TRAF6 ubiquitination and degradation also plays an important role in aseptic periprosthetic loosening process, and LIPUS prevents such loosening by strengthening this pathway.

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“…LIPUS also stimulates the proliferation of induced pluripotent stem cell-derived neural crest stem cells (iPSC–NCSC) [ 33 ]. The proliferative capacity of other cell types, including osteoblasts [ 34 ] and epithelial cells [ 35 ], is also enhanced by LIUS, indicating that this is likely a general phenomenon.…”

Section: Biological Effects Of Ultrasoundmentioning

confidence: 99%

Ultrasound Therapy: Experiences and Perspectives for Regenerative Medicine

Lucas

Pérez

Bernal

et al. 2020

Genes

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Ultrasound has emerged as a novel tool for clinical applications, particularly in the context of regenerative medicine. Due to its unique physico-mechanical properties, low-intensity ultrasound (LIUS) has been approved for accelerated fracture healing and for the treatment of established non-union, but its utility has extended beyond tissue engineering to other fields, including cell regeneration. Cells and tissues respond to acoustic ultrasound by switching on genetic repair circuits, triggering a cascade of molecular signals that promote cell proliferation, adhesion, migration, differentiation, and extracellular matrix production. LIUS also induces angiogenesis and tissue regeneration and has anti-inflammatory and anti-degenerative effects. Accordingly, the potential application of ultrasound for tissue repair/regeneration has been tested in several studies as a stand-alone treatment and, more recently, as an adjunct to cell-based therapies. For example, ultrasound has been proposed to improve stem cell homing to target tissues due to its ability to create a transitional and local gradient of cytokines and chemokines. In this review, we provide an overview of the many applications of ultrasound in clinical medicine, with a focus on its value as an adjunct to cell-based interventions. Finally, we discuss the various preclinical and clinical studies that have investigated the potential of ultrasound for regenerative medicine.

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The effect of low-intensity pulsed ultrasound on wound healing using scratch assay in epithelial cells

Abstract: LIPUS exposure promotes wound closure in the scratch assay and up-regulates the expression level of integrin α6β4 as compared with the control.

Cited by 27 publications

References 38 publications

Low‐intensity pulsed ultrasound affects growth, differentiation, migration, and epithelial‐to‐mesenchymal transition of colorectal cancer cells

Low‐intensity pulsed ultrasound affects growth, differentiation, migration, and epithelial‐to‐mesenchymal transition of colorectal cancer cells

Low-intensity pulsed ultrasound (LIPUS) prevents periprosthetic inflammatory loosening through FBXL2-TRAF6 ubiquitination pathway

Ultrasound Therapy: Experiences and Perspectives for Regenerative Medicine

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