The effect of low-temperature plasma on bacteria as observed by repeated AFM imaging

Pompl, R.; Jamitzky, Ferdinand; Shimizu, Tetsuji; Steffes, B.; Bunk, W.; Schmidt, Hans-Ulrich; Georgi, Matthias; Ramrath, Katrin; Stolz, Wilhelm; Stark, Robert W.; Urayama, Takuya; Fujii, Shuitsu; Morfill, G. E.

doi:10.1088/1367-2630/11/11/115023

Cited by 70 publications

(44 citation statements)

References 16 publications

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“…ROS/RNS can violate the integrity of the microbial cell structure by lipid peroxidation, resulting in membrane damage (6). This has been suggested for plasma-derived ROS/RNS, as well (40). Pompl et al pointed out that the bactericidal effect of CAP is evident before morphological changes indicate cell wall disruption of S. aureus (40).…”

Section: Discussionmentioning

confidence: 89%

“…This has been suggested for plasma-derived ROS/RNS, as well (40). Pompl et al pointed out that the bactericidal effect of CAP is evident before morphological changes indicate cell wall disruption of S. aureus (40). This suggests that a more complex inactivation mechanism exists in which different plasma species may create a synergistic effect by alternating in-cell processes and, thus, are responsible for disinfection.…”

Section: Discussionmentioning

confidence: 94%

See 1 more Smart Citation

Cold Atmospheric Air Plasma Sterilization against Spores and Other Microorganisms of Clinical Interest

Klämpfl

Isbary

Shimizu

et al. 2012

Appl Environ Microbiol

Self Cite

322

202

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dPhysical cold atmospheric surface microdischarge (SMD) plasma operating in ambient air has promising properties for the sterilization of sensitive medical devices where conventional methods are not applicable. Furthermore, SMD plasma could revolutionize the field of disinfection at health care facilities. The antimicrobial effects on Gram-negative and Gram-positive bacteria of clinical relevance, as well as the fungus Candida albicans, were tested. Thirty seconds of plasma treatment led to a 4 to 6 log 10 CFU reduction on agar plates. C. albicans was the hardest to inactivate. The sterilizing effect on standard bioindicators (bacterial endospores) was evaluated on dry test specimens that were wrapped in Tyvek coupons. The experimental D 23°C values for Bacillus subtilis, Bacillus pumilus, Bacillus atrophaeus, and Geobacillus stearothermophilus were determined as 0.3 min, 0.5 min, 0.6 min, and 0.9 min, respectively. These decimal reduction times (D values) are distinctly lower than D values obtained with other reference methods. Importantly, the high inactivation rate was independent of the material of the test specimen. Possible inactivation mechanisms for relevant microorganisms are briefly discussed, emphasizing the important role of neutral reactive plasma species and pointing to recent diagnostic methods that will contribute to a better understanding of the strong biocidal effect of SMD air plasma.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 94%

Cold Atmospheric Air Plasma Sterilization against Spores and Other Microorganisms of Clinical Interest

Klämpfl

Isbary

Shimizu

et al. 2012

Appl Environ Microbiol

Self Cite

322

202

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“…This allows a differentiation by fluorescent signals between healthy and non living microorganisms (LaFlamme et al, 2004). Proof for demolished membranes or other morphological disorders can also be given by electron microscopy (Laroussi et al, 2003) or atomic force microscopy (Pompl et al, 2009;Hähnel et al, 2010a;Kuo et al, 2006 Boucher, 1985;ISO 17665, International Standard, 2006). Generally, microbial control processes should be tested for a variety of microorganism types to ensure process effectivity.…”

Section: Microbiological Verification Of Plasma Effectivitymentioning

confidence: 99%

Low temperature atmospheric pressure plasma sources for microbial decontamination

Ehlbeck¹,

Schnabel²,

Polák³

et al. 2010

J. Phys. D: Appl. Phys.

642

415

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To cite this version:J EhlbeckAbstract. The aim of this article is to provide a survey of plasma sources at atmospheric pressure used for microbicidal treatment. In order to consider the interdisciplinary character of this topic an introduction and definition of basic terms and procedures is given for plasma as well as for microbicidal issues. The list of plasma sources makes no claim to be complete, but to represent the main principles of plasma generation at atmospheric pressure and to give an example of their microbicidal efficiency. The interpretation of the microbicidal results remain difficult due to the non standardized methods uses by different authors and due to the fact that small variations in the set up can change the results dramatically.

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“…It is usually used in medical applications such as wound disinfection. [27,28,40] Here, we are interested in the use of such a device for surface modification because surfaces of arbitrary size can be treated as there is no self-contained reactor. Surface modification effects can be caused by argon ions, ultraviolet radiation, active neutrals, and reactive species generated from the atmosphere during treatment.…”

Section: Plasma Treatmentmentioning

confidence: 99%

“…Thus, we explore hydrophilization with a medical low-temperature atmospheric argon plasma source offering efficient bactericidal effects while having moderate thermal impact. [27,28] Such processes may become relevant for the fabrication and sterilization of biomedical microdevices or implants.…”

Section: Introductionmentioning

confidence: 99%

Surface hydrophilization of SU‐8 by plasma and wet chemical processes

Walther

Drobek

Gigler

et al. 2010

Surface & Interface Analysis

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Wetting properties and surface roughness of SU-8 can be modified by wet chemical and plasma processes. These processes result in an enhanced wettability that can be attributed to an increase of C O and COO groups at the surface. Wet chemical etching with ceric ammonium nitrate (CAN) rendered the SU-8 surface hydrophilic. However, it also led to the accumulation of cerium species on the surface, which may interfere with biochemical reactions in the device. Surface activation could also be achieved with a low-temperature atmospheric argon plasma, which left a smooth surface. Treatment of SU-8 with oxygen plasma led to a stable hydrophilization and an increased surface roughness. Owing to oxygen plasma processing, antimony species were accumulated which could be removed with a cleaning step. In order to maintain the hydrophilization after oxygen plasma treatment for storage, or over several wetting cycles, the surface can be coated with a protein-resistant graft copolymer (PLL-g-PEG).

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The effect of low-temperature plasma on bacteria as observed by repeated AFM imaging

Cited by 70 publications

References 16 publications

Cold Atmospheric Air Plasma Sterilization against Spores and Other Microorganisms of Clinical Interest

Cold Atmospheric Air Plasma Sterilization against Spores and Other Microorganisms of Clinical Interest

Low temperature atmospheric pressure plasma sources for microbial decontamination

Surface hydrophilization of SU‐8 by plasma and wet chemical processes

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