The Effect of Low-Voltage Roentgen Rays on the Normal and Vascularized Cornea of the Rabbit*

Scheie, Harold G.; Dennis, Richard H.; Ripple, Richard C.; Calkins, Larry L.; Buesseler, John A.

doi:10.1016/0002-9394(50)90448-x

Cited by 10 publications

(4 citation statements)

References 17 publications

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“…[12][13][14] Serious injury such as corneal ulcer was produced by doses over 2000 rad (20 Gy). [13][14][15] In the present attempt to elucidate the effect of moderate ionizing radiation on the cornea, we selected a dose of 20 Gy in order to avoid serious corneal damages.…”

Section: Discussionmentioning

confidence: 99%

“…Examinations using a wide-field specular microscope (Keeler-Konan, Tokyo) were conducted before and 1,4,8,12,16,20,24, and 36 weeks afterwards. A drop of sodium hyaluronate was instilled on the cone of the microscope to obtain clear observations of the epithelial and endothelial cells.…”

Section: Examinationsmentioning

confidence: 99%

“…Methods Five healthy mature albino rabbits were unilaterally irradiated with 20 Gy of X-rays (250 kV, 12 mA). Slit-lamp biomicroscopic observation and specular microscopic examination of the corneal epithelium and endothelium were carried out before and 1, 4,8,12,16,20,24, and 36 weeks after irradiation. We evaluated mean area of the superficial corneal epithelial cells, mean area and the percentage of hexagonal cells of the corneal endothelial cells, and corneal thickness.…”

Section: Introductionmentioning

confidence: 99%

“…Most of the previous studies evaluated the corneal epithelium because of its high sensitivity to ionizing radiation. [12][13][14][15] Moreover, histopathological techniques were mainly used in those studies.…”

Section: Introductionmentioning

confidence: 99%

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Specular microscopic study of X-ray-irradiated rabbit cornea

Kiuchi

Tatsuzaki

Hommura

et al. 2004

Eye

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Purpose To investigate the influence of ionizing radiation on the corneal epithelium and endothelium of rabbit eyes. Methods Five healthy mature albino rabbits were unilaterally irradiated with 20 Gy of X-rays (250 kV, 12 mA). Slit-lamp biomicroscopic observation and specular microscopic examination of the corneal epithelium and endothelium were carried out before and 1, 4,8,12,16,20,24, and 36 weeks after irradiation. We evaluated mean area of the superficial corneal epithelial cells, mean area and the percentage of hexagonal cells of the corneal endothelial cells, and corneal thickness. The statistical difference between the irradiated and control eyes was assessed using paired t-test. Results All animals developed cataract within 24 weeks. Slit-lamp biomicroscopy showed no apparent corneal abnormalities over the 36-week follow-up period. Specular microscopy revealed a significant enlargement of the superficial corneal epithelial cells from 4 to 12 weeks after irradiation (Po0.01), which disappeared at 16 weeks post-irradiation. Specular microscopy of the corneal endothelium showed enlargement and morphological alterations of the cells beginning 8 weeks after irradiation (Po0.05). These changes persisted throughout the study period. There were no statistically significant changes in corneal thickness. Conclusion After X-ray radiation of 20 Gy, transient damage occurred in the corneal epithelium, while delayed and irreversible changes were seen in the endothelium.

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