The effect of luteinizing hormone on the ultrastructure of the leydig cell of the chick

Connell, Carolyn J.

doi:10.1007/bf00306894

Cited by 44 publications

(16 citation statements)

References 28 publications

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“…In its nucleus (N) two spheridies (arrows) and nucleoli (NL) are seen. The arrow-head indicates a filopodium of the Leydig cell taken up in a coated invagination forms have been confirmed in a variety of species (BELT andCAVAZOS, 1967, 1971;DE KRETSER, 1967a;SINHA and SEAL, 1967;CHRISTENSEN, 1970CHRISTENSEN, , 1975CONNELL, 1972;USUI, 1976;WING and LIN, 1977). Distinction between tubular and lamellar cristae in a single section was not always easy, and this apparently caused different conclusions reached by different authors in the same animal species-YAMADA (1965), NAGANO (1965) and DE KRETSER (1967b) vs. DE KRETSER (1967a) and CHRISTENSEN (1970; SCHWARZ and MERKER (1965) and USUI (1976) vs. MURAKAMI (1966) and LORDING and DE KRETSER (1972.…”

Section: Mitochondriamentioning

confidence: 89%

“…A mitotic figure of the macrophage has been detected. CHRISTENSEN and FAWCETT, 1961;LEESON, 1963;CRABO, 1963;CHRISTENSEN, 1965CHRISTENSEN, , 1970MURAKAMI, 1966;DE KRETSER, 1967a, b;CONNELL, 1972;USUI, 1976). CHRISTENSEN (1965) assumed that the occurrence of dark and light cells might be an artifact induced by unsuitable fixation.…”

Section: Macrophagementioning

confidence: 99%

“…The vesicular SER, however, have been thought to be an artifact produced by OsO4 fixation or other inadequate techniques (FAWCETT and BURGOS, 1960;CRABO, 1963;CHRISTENSEN, 1965;NAGANO, 1965;BELT and CAVAZOS, 1967;SINHA and SEAL, 1969). Although CHRISTENSEN (1970) persisted that in human testes perfusion-fixed with glutaraldehyde followed by immersion fixation with OsO4, interstitial cells exclusively contained tubular SER, many authors actually revealed interstitial cells with the vesicular SER along with those with the tubular one in the same specimen treated by the same fixatives (MURAKAMI, 1966;DE KRETSER, 1967a, b;CONNELL, 1972;USUI, 1976). YAMADA (1965) and the present author found that the interstitial cells containing the tubular and those containing the vesicular reticulum, coexisted side by side in the same section, and subsequently concluded that the different appearances of the SER might represent a functional variation of the interstitial cell and not an artifact.…”

Section: Sermentioning

confidence: 99%

“…In adult mouse testis examined in this study, foci of RER were distributed rather randomly in the cytoplasm, while free ribosomes, mostly forming polysomes were distributed not only in the patches of the RER, but also throughout the entire cytoplasm among smooth membranes. Developmental electron microscopic studies on Leydig cells carried out by BLACK and CHRISTENSEN After administration of human chorionic gonadotropin (HCG) or luteinizing hormone (LH), increase in the foci of dilated RER cisternae and in the free ribosomes in the Leydig cell have been observed immature and mature animals (MERKOW et al, 1968a, b;AOKI and MASSA, 1972;CONNELL, 1972). MERKOW et al (1968a) andCONNELL (1972) have related these progressive changes in the RER and free ribosomes to increased production of protein, carrier protein and enzymes necessary for the steroid synthesis, cell growth and new membrane formation.…”

Section: Rer and Free Ribosomesmentioning

confidence: 99%

“…In a variety of animal species including human, several authors described both or one of these inclusions (CHRISTENSEN and FAWCETT, 1961;LEESON, 1963;YAMADA, 1955;MURAKAMI, 1966;BELT and CAVAZOS, 1967;DE KRETSER, 1967a;BLACK and CHRISTENSEN, 1969;SINHA and SEAL, 1969;CHRISTENSEN, 1970;CONNELL, 1972;USUI, 1976). CHRISTENSEN (1970) assumed that large spherical osmiophilic inclusions (lipid droplets) might be a pool of pregnenolone.…”

Section: Mitochondriamentioning

confidence: 99%

See 4 more Smart Citations

Electron Microscopic Study on the Testicular Interstitial Cells in the Mouse

Ohata

1979

Arch. Histol. Jap., Arch. Histol. Jpn

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Section: Mitochondriamentioning

confidence: 89%

Section: Macrophagementioning

confidence: 99%

Section: Sermentioning

confidence: 99%

Section: Rer and Free Ribosomesmentioning

confidence: 99%

Section: Mitochondriamentioning

confidence: 99%

See 3 more Smart Citations

Electron Microscopic Study on the Testicular Interstitial Cells in the Mouse

Ohata

1979

Arch. Histol. Jap., Arch. Histol. Jpn

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Postnatal differentiation of leydig cells in the rabbit testis

1976

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After the gradual disappearance of fetal Leydig cells in the first week after birth, the interstitial tissue of the rabbit testis is composed principally of undifferentiated mesenchymal cells between one and five weeks of age. Also present during this time are scattered partially differentiated cells with oval-shaped nuclei, prominent nucleoli and abundant cytoplasm. These cells exhibit some of the cytoplasmic features of steroid-secreting cells, but extensive development of smooth endoplasmic reticulum and the grouped perivascular arrangement of characteristic of fully differentiated Leydig cells are not present. The latter features appear at five weeks of age, indicating the formation of mature Leydig cells at that time. By seven weeks, the bulk of the interstitial tissue consists of Leydig cell aggregates, typical of the appearance in adult testis. Since spermatogonial mitoses first appear at seven to eight weeks of age, the findings indicate that Leydig cell differentiation precedes the onset of spermatogenesis.

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Changes in leydig cell activity during the annual testicular cycle of the bat Myotis lucifugus lucifugus: Histology and lipid histochemistry

Gustafson

1987

Am. J. Anat.

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Changes in Leydig cell histology and testicular sudanophilic lipids were examined in relation to spermatogenic activity in the bat Myotis lucifugus lucifugus (Chiroptera: Vespertilionidae) throughout the annual cycle in the northeastern United States. These changes were correlated with annual variations in plasma testosterone concentrations which have recently been described for this species. Gametogenic activity occurred during the months of May-August when bats were metabolically most active. During hibernation (October-April), when sperm are stored in the epididymides, and accessory glands are hypertrophic, the seminiferous tubules were at rest, and the germinal epithelium was reduced to reserve spermatogonia and Sertoli cells. Based on their structure and cyclic pattern of sudanophilic lipids, Leydig cells exhibited a pattern of activity that closely paralleled that of the seminiferous epithelium. On renewal of spermatogenesis in spring, Leydig cells became hypertrophied and accumulated lipid inclusions. These inclusions, seen as vacuoles in plastic sections and sudanophilic droplets in frozen sections, reached maximal accumulations in late June. In late July and during August, when peak testosterone levels occur in blood, lipid droplets were dramatically depleted, and Leydig cells were weakly sudanophilic. In September, when testosterone titers return to low baseline levels, Leydig cells had regressed but exhibited a marked increase in sudanophilic inclusions which appeared to be mostly lipofuscins. During the ensuing mating and hibernation periods, Leydig cells were involuted and filled with lipofuscins. During the periarousal period, however, Leydig cells became weakly Sudan-positive while many large, intensely sudanophilic cells were scattered throughout the interstitium. In electron micrographs these cells were identified as macrophages. They appear to play an important role in the annual testicular cycle by phagocytizing the residues of Leydig cell involution in preparation for a new steroidogenic cycle. Seasonal changes in lipid inclusions were also observed in the seminiferous tubules. In addition, the relationship of the Leydig cell cycle to androgen action and the accessory organs in this bat is discussed.

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The effect of luteinizing hormone on the ultrastructure of the leydig cell of the chick

Cited by 44 publications

References 28 publications

Electron Microscopic Study on the Testicular Interstitial Cells in the Mouse

Electron Microscopic Study on the Testicular Interstitial Cells in the Mouse

Postnatal differentiation of leydig cells in the rabbit testis

Changes in leydig cell activity during the annual testicular cycle of the bat Myotis lucifugus lucifugus: Histology and lipid histochemistry

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