The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors

Jiráková, Klára; Šeneklová, Monika; Jirák, Daniel; Turnovcová, Karolína; Vosmanská, Magda; Babič, Michal; Horák, Daniel; Veverka, Pavel; Jendelová, Pavla

doi:10.2147/ijn.s116171

Cited by 17 publications

(11 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…14,21,[27][28][29]37 The in vivo animal model provided further toxicity information where 3 out of 9 of the in vivo animal model studies indicated partial nontoxic animal effect, one was toxic while the remaining results recommended ENPBCs as a potential candidate for drug therapy with limited information on toxicity (Table S2). Some of the in vivo ENPBCs toxicity effects demonstrated programmed cell death, causing the release of H 2 S. 29 The ENPBCs that exhibited in vivo nontoxic effect were mostly those that had the therapeutic effects of antibody-drug conjugate, 27 CTAB layers of gold for diagnosing rheumatoid arthritis, 30 surface coating polymeric NPs used for inhibiting cancerous cells 31,[38][39][40][41][42][43][44][45][46][47] and felodipine-loaded polymers for pathological examination of different organs of Wister albino mice 50. The ENPBCs with partial nontoxic effect were chitosan-coated polymers for drug delivery, 49 silver NPs coated polymers that were found localized in various body organs, 48 and drug delivery polymeric NPs for anticancer. 51 The findings indicated limited data and information regarding the regulation of ENPBCs toxicity.…”

Section: Resultsmentioning

confidence: 99%

Engineered nanoparticle bio-conjugates toxicity screening: The xCELLigence cells viability impact

Yah

Simate

2020

Bioimpacts

View full text Add to dashboard Cite

Introduction: The vast diverse products and applications of engineered nanoparticle bio-conjugates (ENPBCs) are increasing, and thus flooding the-markets. However, the data to support risk estimates of ENPBC are limited. While it is important to assess the potential benefits, acceptability and uptake, it is equally important to understand where ENPBCs safety is and how to expand and affirm consumer security concerns. Methods: Online articles were extracted from 2013 to 2016 that pragmatically used xCELLigence real-time cell analysis (RTCA) technology to describe the in-vitro toxicity of ENPBCs. The xCELLigence is a +noninvasive in vitro toxicity monitoring process that mimics exact continuous cellular bio-responses in real-time settings. On the other hand, articles were also extracted from 2008 to 2016 describing the in vivo animal models toxicity of ENPBCs with regards to safety outcomes. Results: Out of 32 of the 121 (26.4%) articles identified from the literature, 23 (71.9%) met the in-vitro xCELLigence and 9(28.1%) complied with the in vivo animal model toxicity inclusion criteria. Of the 23 articles, 4 of them (17.4%) had no size estimation of ENPBCs. The xCELLigence technology provided information on cell interactions, viability, and proliferation process. Eighty-three (19/23) of the in vitro xCELLigence technology studies described ENPBCs as nontoxic or partially nontoxic materials. The in vivo animal model provided further toxicity information where 1(1/9) of the in vivo animal model studies indicated potential animal toxicity while the remaining results recommended ENPPCs as potential candidates for drug therapy though with limited information on toxicity. Conclusion: The results showed that the bioimpacts of ENPBCs either at the in vitro or at in vivo animal model levels are still limited due to insufficient information and data. To keep pace with ENPBCs biomedical products and applications, in vitro, in vivo assays, clinical trials and long-term impacts are needed to validate their usability and uptake. Besides, more real-time ENPBCs-cell impact analyses using xCELLigence are needed to provide significant data and information for further in vivo testing.

show abstract

Section: Resultsmentioning

confidence: 99%

Engineered nanoparticle bio-conjugates toxicity screening: The xCELLigence cells viability impact

Yah

Simate

2020

Bioimpacts

View full text Add to dashboard Cite

show abstract

“…Cells were then cultivated under standard cultivation conditions. Impedance was recorded every 15 minutes for 48 hours, with each experiment performed in doublets …”

Section: Methodsmentioning

confidence: 99%

Manganese‐Zinc Ferrites: Safe and Efficient Nanolabels for Cell Imaging and Tracking In Vivo

et al. 2019

View full text Add to dashboard Cite

Manganese‐zinc ferrite nanoparticles were synthesized by using a hydrothermal treatment, coated with silica, and then tested as efficient cellular labels for cell tracking, using magnetic resonance imaging (MRI) in vivo. A toxicity study was performed on rat mesenchymal stem cells and C6 glioblastoma cells. Adverse effects on viability and cell proliferation were observed at the highest concentration (0.55 mM) only; cell viability was not compromised at lower concentrations. Nanoparticle internalization was confirmed by transmission electron microscopy. The particles were found in membranous vesicles inside the cytoplasm. Although the metal content (0.42 pg Fe/cell) was lower compared to commercially available iron oxide nanoparticles, labeled cells reached a comparable relaxation rate R 2, owing to higher nanoparticle relaxivity. Cells from transgenic luciferase‐positive rats were used for in vivo experiments. Labeled cells were transplanted into the muscles of non‐bioluminescent rats and visualized by MRI. The cells produced a distinct hypointense signal in T2‐ or T2*‐weighted MR images in vivo. Cell viability in vivo was verified by bioluminescence.

show abstract

“…Comparison between two iron-based nanoparticles contrast agents, silica-coated cobalt zinc ferrite nanoparticles (CZF) and poly-l-lysine-coated iron oxide superparamagnetic nanoparticles (PLL-coated γ-Fe 2 O 3 ) revealed that PLL-coated γ-Fe 2 O 3 would not affect cell proliferation, while CZF would slow down this process. However, no significant differences in neural differentiation were observed between unlabeled cells or cells labeled with both magnetic nanoparticles [82]. Thus, MRI-based stem-cell tracking may offer a novel practical method to follow transplanted stem-cell therapy for neurological disorders.…”

Section: Nanotechnology Application For Monitoring Stem-cell Therapy mentioning

confidence: 97%

Application of Nanotechnology in Stem-Cell-Based Therapy of Neurodegenerative Diseases

et al. 2020

View full text Add to dashboard Cite

In addition to adverse health outcomes, neurological disorders have serious societal and economic impacts on patients, their family and society as a whole. There is no definite treatment for these disorders, and current available drugs only slow down the progression of the disease. In recent years, application of stem cells has been widely advanced due to their potential of self-renewal and differentiation to different cell types which make them suitable candidates for cell therapy. In particular, this approach offers great opportunities for the treatment of neurodegenerative disorders. However, some major issues related to stem-cell therapy, including their tumorigenicity, viability, safety, metastases, uncontrolled differentiation and possible immune response have limited their application in clinical scales. To address these challenges, a combination of stem-cell therapy with nanotechnology can be a solution. Nanotechnology has the potential of improvement of stem-cell therapy by providing ideal substrates for large scale proliferation of stem cells. Application of nanomaterial in stem-cell culture will be also beneficial to modulation of stem-cell differentiation using nanomedicines. Nanodelivery of functional compounds can enhance the efficiency of neuron therapy by stem cells and development of nanobased techniques for real-time, accurate and long-lasting imaging of stem-cell cycle processes. However, these novel techniques need to be investigated to optimize their efficiency in treatment of neurologic diseases.

show abstract

The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors

Cited by 17 publications

References 49 publications

Engineered nanoparticle bio-conjugates toxicity screening: The xCELLigence cells viability impact

Engineered nanoparticle bio-conjugates toxicity screening: The xCELLigence cells viability impact

Manganese‐Zinc Ferrites: Safe and Efficient Nanolabels for Cell Imaging and Tracking In Vivo

Application of Nanotechnology in Stem-Cell-Based Therapy of Neurodegenerative Diseases

Contact Info

Product

Resources

About