The effect of magnetic nanoparticles containing hyaluronic acid and methotrexate on the expression of genes involved in apoptosis and metastasis in A549 lung cancer cell lines

Dou, Jiatai; Mi, Yanfei; Daneshmand, Sara; Majd, Mostafa Heidari

doi:10.1016/j.arabjc.2022.104307

Cited by 18 publications

(7 citation statements)

References 52 publications

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“…The surface charges of these nanoconjugates also show the +45.7 and -27.8 mV confirming the successful functionalization with NH2 groups of PEI and -COOH groups of HA molecules. The decrease of zeta potential of the MNPs from +45.7 to -27 indicates the free amine groups of PEI-MNPs should be protonated and hence positive charged at neutral pH while after modification with HA, as HA is negatively charged -COOH groups and -CONH groups due to covalent bonding, this rendered HA-PEI-MNPs bearing negative zeta potential [36,37]. The successful functionalization with HA was confirmed by Fourier transform infrared (FTIR) spectroscopy.…”

Section: Resultsmentioning

confidence: 94%

Development of hyaluronate tethered magnetic nanoparticlesfor targeted anti-cancer drug delivery

Bhattacharya

2024

Zas Mat

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Despite the tremendous progress in understanding the molecular basis of the disease, cancer still remains one of the leading causes of deaths. Recently, advances in nanotechnology are rapidly enabling the development of novel, multifunctional materials with combined cancer specific targeting, therapeutic and diagnostic functions within a single nanocomplex (NP) that address the shortcomings of traditional disease diagnostic and therapeutic agents. Among the myriad of nanocarriers, magnetic nanoparticles (MNPs) have sparked extensive promise as novel theranostic applications as these MNPs can be directly targeted to the diseased cells with effective therapeutic efficiency. For this, these MNPs should be modified with some highly biocompatible polymers (specially polysaccharides) exhibiting the cancer targeting properties that can strongly interact with receptors expressed on the target cancer to facilitate accurate detection of the specific cancer and enhanced delivery to the target site while reducing unintended tside effects. Over the last few years, many groups have reported hyaluronic acid (HA) as the targeting agent as it directly delivers targeted MNPs to CD44 overexpressed cancer cells. In most of the cases, doxorubicin (DOX) has been used as the anticancer drug as it is largely utilized for treating a broad spectrum of cancers. In our work, we have designed a novel, intravenously injectable, CD44 receptor targeted MNP formulation, where the HA moiety of MNPs facilitate easy detection of cancer cells via receptor specific interactions, DOX can regress the cancer cells with simultaneous imaging efficacy. This theranostic MNPs led to the formation of novel nanoformulation, capable of performing concomitant detection, regression and imaging in in vitro CD44 over expressing cancer cells.

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Section: Resultsmentioning

confidence: 94%

Development of hyaluronate tethered magnetic nanoparticlesfor targeted anti-cancer drug delivery

Bhattacharya

2024

Zas Mat

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“…Other steps of RNA isolation were performed according to the manufacturer’s protocol of the High Pure RNA Isolation Kit, which were fully described in our previous works 17 – 19 . Also, the details of cDNA synthesis using the cDNA synthesis kit were mentioned in our previous work 20 . The expression of the genes listed in Table 1 was measured using the Real-time PCR method and according to the appropriate thermal cycle by the Applied Biosystems StepOne™ device (Thermo Fisher Scientific, Massachusetts, USA) 17 .…”

Section: Methodsmentioning

confidence: 99%

Synthesis of halloysite nanotubes decorated with green silver nanoparticles to investigate cytotoxicity, lipid peroxidation and induction of apoptosis in acute leukemia cells

Zhang,

Heidari Majd

2023

Sci Rep

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Leukemia is the 15th most common cancer in adults and the first most common cancer in children under the age of five, and unfortunately, it accounts for many deaths every year. Since leukemia chemotherapy usually fails due to chemotherapy resistance and disease relapse, many efforts are being made to develop new methods of leukemia treatment. Therefore, for the first time, we decorated halloysite nanotubes (HNTs) with green silver nanoparticles (Ag NPs) with the help of Moringa Peregrina leaves extract to increase the solubility of Ag NPs and to use the protective ability of HNTs against lipid peroxidation in erythrocytes. Cell survival assay by the MTT method showed that HNTs-Ag NPs can decrease the survival of Jurkat T-cells to about 10% compared to the control. The IC50 value was estimated as 0.00177 mg/mL after 96 h of treatment. Investigating the expression of genes involved in apoptosis by Real-time PCR proved that decorated HNTs with Ag NPs can increase the Bak1/Bclx ratio by 17.5 times the control group. Also, the expression of the caspase-3 gene has increased 10 times compared to the control. Finally, the reduction of malondialdehyde production after 24 h proved that the presence of HNTs can have a good protective effect on lipid peroxidation in erythrocytes. Therefore, on the one hand, we can hope for the ability of HNTs-Ag NPs to induce apoptosis in blood cancer cells and on the other hand for its protective effects on normal blood cells.

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“…Active targeting was also employed for MTX delivery, using HA [ 169 ] or FA antibodies [ 170 ] as targeting agents. The HA-modified DDS displayed high drug encapsulation efficiency and the drug release was pH-dependent, with a higher than 10-fold increase in released MTX at pH 5.5 compared to pH 7.4.…”

Section: Chemotherapy Delivery Using Magnetic Nanoparticlesmentioning

confidence: 99%

“…Moreover, the DDS caused an increase in the expression of pro-apoptotic genes in cancer cells and a decrease in the expression of the gene encoding the pro-inflammatory cytokine tumor necrosis factor α (TNF-α). This demonstrates that the DDS has potential anticancer activity in A549 lung cancer cells, a type of cell that has high resistance to MTX [ 169 ]. Antibodies against folate receptors were synthesized and conjugated to MTX-modified magnetic NPs.…”

Section: Chemotherapy Delivery Using Magnetic Nanoparticlesmentioning

confidence: 99%

Recent Advances in the Development of Drug Delivery Applications of Magnetic Nanomaterials

et al. 2023

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With the predicted rise in the incidence of cancer, there is an ever-growing need for new cancer treatment strategies. Recently, magnetic nanoparticles have stood out as promising nanostructures for imaging and drug delivery systems as they possess unique properties. Moreover, magnetic nanomaterials functionalized with other compounds can lead to multicomponent nanoparticles with innovative structures and synergetic performance. The incorporation of chemotherapeutic drugs or RNA in magnetic drug delivery systems represents a promising alternative that can increase efficiency and reduce the side effects of anticancer therapy. This review presents a critical overview of the recent literature concerning the advancements in the field of magnetic nanoparticles used in drug delivery, with a focus on their classification, characteristics, synthesis and functionalization methods, limitations, and examples of magnetic drug delivery systems incorporating chemotherapeutics or RNA.

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The effect of magnetic nanoparticles containing hyaluronic acid and methotrexate on the expression of genes involved in apoptosis and metastasis in A549 lung cancer cell lines

Cited by 18 publications

References 52 publications

Development of hyaluronate tethered magnetic nanoparticlesfor targeted anti-cancer drug delivery

Development of hyaluronate tethered magnetic nanoparticlesfor targeted anti-cancer drug delivery

Synthesis of halloysite nanotubes decorated with green silver nanoparticles to investigate cytotoxicity, lipid peroxidation and induction of apoptosis in acute leukemia cells

Recent Advances in the Development of Drug Delivery Applications of Magnetic Nanomaterials

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