The Effect of Male Mating Competitiveness, Developmental Rate, and Viability of Larvae and Pupae in Drosophila melanogaster Heterozygous for the Temperature-Sensitive Lethal Mutation l(2)M167 DTS on the Dynamics of the Mutation Elimination from the Population

Abstract

The dependence of selection on an introduced mutation l(2)M167 DTS on male mating competitiveness, viability, and developmental rate of larvae and pupae of Drosophila melanogaster , heterozygous for this mutation, was examined in population experiments with preset conditions. The limitations of fitness parameters of individuals l(2)M167 DTS /+ relative to individuals +/+ were estimated according to the conditions of the experiment and phenotypic characteristics of the mutation studied. Under conditions of limited food supply and dependence on emergence time in each generation, the sequence of female mating was shown to be of less importance than the order of medium utilization by the progeny of a certain genotype related to the male success in the first mating. The limiting factors acting on the l(2)M167 DTS mutation were viability and developmental rate. GENERAL GENETICS

Selection on Viability of Individuals Heterozygous for the Temperature-Sensitive Lethal Mutation l(2)M167 DTS in Experimental Populations of Drosophila melanogaster

“…This does not correspond to the observed frequencies of l167/+ individuals in the total progeny of the parental females from population 1, obtained out of the experimental populations, at the third significance level. In [20], we have considered the effect of male mating competitiveness as a fitness component on the efficiency of introduction of the l167 mutation in the experimental population (determining the observed effects). Here, we would like to emphasize that the similarity of genotype segregations in the progeny of males obtained out of the experimental populations 1 and 2 and the increase in the proportion of heterozygous males in the progeny of males l167/+ from the subsequent generations suggest differential action of different fitness components at different stages of mutation selection.…”

“…We have demonstrated that the limiting factors determining intensity of selection on mutation l167 were viability and developmental rate, while male mating competitiveness can enhance fitness of the mutation carriers [20]. It is also known that individuals l167 /+ exhibit polymorphism associated with the presence of some modification systems exactly for embryonic, larval, and pupal viability [21].…”

Determination of Fitness Components of Flies Bearing the Recessive Lethal l(2)M167 DTS Mutation with Dominant Heat Sensitivity in Artificial Drosophila melanogaster Populations

“…By using estimates of fitness components s , w , and m reported in [8][9][10], we managed to narrow the range calculated according to elimination patterns and thereby describe the interrelationships between the unknown components f and w with other components specified. We applied the following limitations on particular fitness components obtained in earlier tests:…”

“…In the experimental populations, it is highly likely to vary within 1.0-1.5 with reference to +/+ males; i.e., m l167/+ ≥ m +/+ [10].…”

“…The method involves numerical solution of the model and description of the range of admissible values of specified fitness components with regard to reasonable limitations imposed on some components, e.g., according to the results of preliminary estimation of the variability ranges of these components. The values of fitness components were determined in [8][9][10][11][12]. The model was also modified to fit the case of asymmetry related to the limited fertility of females heterozygous for the mutation.…”