The effect of mannosylation of liposome-encapsulated indocyanine green on imaging of sentinel lymph node

Jeong, Hwan-Seok; Lee, Chang-Moon; Cheong, Su-Jin; Kim, Eun‐Mi; Hwang, Hyeon-Shik; Na, Kyung Sook; Lim, Sungjoon; Sohn, Myung‐Hee; Jeong, Hwan-Jeong

doi:10.3109/08982104.2013.801488

Cited by 33 publications

(24 citation statements)

References 35 publications

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“…Previously, liposome systems labeled with ICG have been considered as fluorescent agents for intradermal administration in the footpad region of healthy animals, intended for lymph-node imaging [18,23,24]. The LipoICG vesicles in this study resolve the two main limitations of free ICG for such clinical applications [41,42], namely its low stability in aqueous environments and its strong binding to serum albumin that leads to a dramatic reduction of optical signal after intra-venous injection.…”

Section: Discussionmentioning

confidence: 92%

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Dynamic imaging of PEGylated indocyanine green (ICG) liposomes within the tumor microenvironment using multi-spectral optoacoustic tomography (MSOT)

et al. 2015

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Section: Discussionmentioning

confidence: 92%

“…Liposome systems have been labeled with ICG previously [18,23,24] however solely as a fluorescent probe and only for subcutaneous or intradermal administration, intended mainly for lymph-node imaging. Moreover, there is very limited information on the methodology of ICG incorporation into liposomal bilayers.…”

Section: Introductionmentioning

confidence: 99%

Dynamic imaging of PEGylated indocyanine green (ICG) liposomes within the tumor microenvironment using multi-spectral optoacoustic tomography (MSOT)

et al. 2015

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“…This permits high NP dye loadings (>50-75% mass) with high PA contrast, when compared to liposomal NP systems whose loads with hydrophobic dyes are limited to surface conjugation or intercalation within membrane bilayers. [26][27][28][29] Interior dye encapsulation also permits the tuning of surface properties such as targeting and clearance, simply by choice of stabilizer properties. Dense polyethylene glycol (PEG) steric layers minimize protein adsorption and slow reticuloendothelial clearance.…”

Section: Introductionmentioning

confidence: 99%

Narrow Absorption NIR Wavelength Organic Nanoparticles Enable Multiplexed Photoacoustic Imaging

Wilson

Heinmiller

et al. 2016

ACS Appl. Mater. Interfaces

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Photoacoustic (PA) imaging is an emerging hybrid optical-ultrasound based imaging technique that can be used to visualize optical absorbers in deep tissue. Free organic dyes can be used as PA contrast agents to concurrently provide additional physiological and molecular information during imaging, but their use in vivo is generally limited by rapid renal clearance for soluble dyes and by the difficulty of delivery for hydrophobic dyes. We here report the use of the block copolymer directed self-assembly process, Flash NanoPrecipitation (FNP), to form series of highly hydrophobic optical dyes into stable, biocompatible, and water-dispersible nanoparticles (NPs) with sizes from 38 to 88 nm and with polyethylene glycol (PEG) surface coatings suitable for in vivo use. The incorporation of dyes with absorption profiles within the infrared range, that is optimal for PA imaging, produces the PA activity of the particles. The hydrophobicity of the dyes allows their sequestration in the NP cores, so that they do not interfere with targeting, and high loadings of >75 wt % dye are achieved. The optical extinction coefficients (ε (mL mg(-1) cm(-1))) were essentially invariant to the loading of the dye in NP core. Co-encapsulation of dye with vitamin E or polystyrene demonstrates the ability to simultaneously image and deliver a second agent. The PEG chains on the NP surface were functionalized with folate to demonstrate folate-dependent targeting. The spectral separation of different dyes among different sets of particles enables multiplexed imaging, such as the simultaneous imaging of two sets of particles within the same animal. We provide the first demonstration of this capability with PA imaging, by simultaneously imaging nontargeted and folate-targeted nanoparticles within the same animal. These results highlight Flash NanoPrecipitation as a platform to develop photoacoustic tools with new diagnostic capabilities.

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“…Binding of ICG to the lipid has been assumed through indirect evidence to stabilize ICG fluorescence and protect the ICG molecule from oxidation, resulting in an enhanced fluorescence intensity. 4,9,13−16 However, the interactions between ICG and lipid have yet to be studied.…”

mentioning

confidence: 99%

Interactions of Indocyanine Green and Lipid in Enhancing Near-Infrared Fluorescence Properties: The Basis for Near-Infrared Imaging in Vivo

2014

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Indocyanine green (ICG) is a near-infrared (NIR) contrast agent commonly used for in vivo cardiovascular and eye imaging. For medical diagnosis, ICG is limited by its aqueous instability, concentration-dependent aggregation, and rapid degradation. To overcome these limitations, scientists have formulated ICG in various liposomes, which are spherical lipid membrane vesicles with an aqueous core. Some encapsulate ICG, while others mix it with liposomes. There is no clear understanding of lipid–ICG interactions. Therefore, we investigated lipid–ICG interactions by fluorescence and photon correlation spectroscopy. These data were used to design stable and maximally fluorescent liposomal ICG nanoparticles for NIR optical imaging of the lymphatic system. We found that ICG binds to and is incorporated completely and stably into the lipid membrane. At a lipid:ICG molar ratio of 250:1, the maximal fluorescence intensity was detected. ICG incorporated into liposomes enhanced the fluorescence intensity that could be detected across 1.5 cm of muscle tissue, while free ICG only allowed 0.5 cm detection. When administered subcutaneously in mice, lipid-bound ICG in liposomes exhibited a higher intensity, NIR image resolution, and enhanced lymph node and lymphatic vessel visualization. It also reduced the level of fluorescence quenching due to light exposure and degradation in storage. Lipid-bound ICG could provide additional medical diagnostic value with NIR optical imaging for early intervention in cases of lymphatic abnormalities.

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The effect of mannosylation of liposome-encapsulated indocyanine green on imaging of sentinel lymph node

Cited by 33 publications

References 35 publications

Dynamic imaging of PEGylated indocyanine green (ICG) liposomes within the tumor microenvironment using multi-spectral optoacoustic tomography (MSOT)

Dynamic imaging of PEGylated indocyanine green (ICG) liposomes within the tumor microenvironment using multi-spectral optoacoustic tomography (MSOT)

Narrow Absorption NIR Wavelength Organic Nanoparticles Enable Multiplexed Photoacoustic Imaging

Interactions of Indocyanine Green and Lipid in Enhancing Near-Infrared Fluorescence Properties: The Basis for Near-Infrared Imaging in Vivo

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