The effect of matrix stiffness of injectable hydrogels on the preservation of cardiac function after a heart attack

Plotkin, Marian; Vaibavi, Srirangam Ramanujam; Rufaihah, Abdul Jalil; Nithya, Venkateswaran; Wang, Jing; Shachaf, Yonatan; Kofidis, Theo; Seliktar, Dror

doi:10.1016/j.biomaterials.2013.10.058

Cited by 85 publications

(66 citation statements)

References 40 publications

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“…As can be seen, a large injectate occupies more than half of the LV wall thickness without creating any bulging on the epicardial or endocardial surface. This finding is at odds with our own in vivo measurements of the same subjects (Choy et al, 2018) and multiple studies that found increases in wall thickness due to biomaterial injection therapy (Christman et al, 2004; Landa et al, 2008; Plotkin et al, 2014; Kadner et al, 2012; Dobner et al, 2009; Sabbah et al, 2013; Leor et al, 2009). These differences in in vivo and ex vivo findings may reveal an important mechanism of the treatment.…”

Section: Discussioncontrasting

confidence: 87%

“…Injectable biomaterials have shown promise in pre-clinical studies resulting in a range of improvements including increased ventricular wall thickness (Christman et al, 2004; Landa et al, 2008; Plotkin et al, 2014; Kadner et al, 2012; Dobner et al, 2009; Sabbah et al, 2013; Leor et al, 2009), increased scar thickness (Landa et al, 2008; Kadner et al, 2012; Leor et al, 2009), higher ejection fractions (Christman et al, 2004; Landa et al, 2008; Plotkin et al, 2014; Kadner et al, 2012; Sabbah et al, 2013) and decreased ventricular dilation (Christman et al, 2004; Landa et al, 2008; Plotkin et al, 2014; Kadner et al, 2012; Dobner et al, 2009; Sabbah et al, 2013; Leor et al, 2009). Alginate injections are known to have nonthrombogenic properties (Lee and Mooney, 2001; Cabrales et al, 2005) in addition to mitigating the effects of adverse ventricular remodelling following MI, which makes them a particularly viable potential therapy.…”

Section: Introductionmentioning

confidence: 99%

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Effect of intra-myocardial Algisyl-LVR™ injectates on fibre structure in porcine heart failure

Sack

Aliotta

Choy

et al. 2018

Journal of the Mechanical Behavior of Biomedical Materials

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Recent preclinical trials have shown that alginate injections are a promising treatment for ischemic heart disease. Although improvements in heart function and global structure have been reported following alginate implants, the underlying structure is poorly understood. Using high resolution ex vivo MRI and DT-MRI of the hearts of normal control swine (n = 8), swine with induced heart failure (n = 5), and swine with heart failure and alginate injection treatment (n =6), we visualized and quantified the fibre distribution and implant material geometry. Our findings show that the alginate injectates form solid ellipsoids with a retention rate of 68.7% ± 21.3% (mean ± SD) and a sphericity index of 0.37 ± 0.03. These ellipsoidal shapes solidified predominantly at the mid-wall position with an inclination of −4.9° ± 31.4° relative to the local circumferential direction. Overall, the change to left ventricular wall thickness and myofiber orientation was minor and was associated with heart failure and not the presence of injectates. These results show that alginate injectates conform to the pre-existing tissue structure, likely expanding along directions of least resistance as mass is added to the injection sites. The alginate displaces the myocardial tissue predominantly in the longitudinal direction, causing minimal disruption to the surrounding myofiber orientations.

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Section: Discussioncontrasting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Effect of intra-myocardial Algisyl-LVR™ injectates on fibre structure in porcine heart failure

Sack

Aliotta

Choy

et al. 2018

Journal of the Mechanical Behavior of Biomedical Materials

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“…Rheological measurements of PF hydrogels were carried out using AR-G2 rheometer (TA instruments, New Castle, DE, USA) according to a published protocol [14]. Briefly, after 1 min equilibrium time, 200 μ L PF precursor solution loaded on the instrument was cross-linked by exposure to long-wave UV light (365 nm) emitted from OmniCure Series 2000 UV light source at an intensity of 5 mW/cm 2 .…”

Section: Methodsmentioning

confidence: 99%

Modulation of Dental Pulp Stem Cell Odontogenesis in a Tunable PEG-Fibrinogen Hydrogel System

Pandya

Rufaihah

et al. 2015

Stem Cells International

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Injectable hydrogels have the great potential for clinical translation of dental pulp regeneration. A recently developed PEG-fibrinogen (PF) hydrogel, which comprises a bioactive fibrinogen backbone conjugated to polyethylene glycol (PEG) side chains, can be cross-linked after injection by photopolymerization. The objective of this study was to investigate the use of this hydrogel, which allows tuning of its mechanical properties, as a scaffold for dental pulp tissue engineering. The cross-linking degree of PF hydrogels could be controlled by varying the amounts of PEG-diacrylate (PEG-DA) cross-linker. PF hydrogels are generally cytocompatible with the encapsulated dental pulp stem cells (DPSCs), yielding >85% cell viability in all hydrogels. It was found that the cell morphology of encapsulated DPSCs, odontogenic gene expression, and mineralization were strongly modulated by the hydrogel cross-linking degree and matrix stiffness. Notably, DPSCs cultured within the highest cross-linked hydrogel remained mostly rounded in aggregates and demonstrated the greatest enhancement in odontogenic gene expression. Consistently, the highest degree of mineralization was observed in the highest cross-linked hydrogel. Collectively, our results indicate that PF hydrogels can be used as a scaffold for DPSCs and offers the possibility of influencing DPSCs in ways that may be beneficial for applications in regenerative endodontics.

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“…The hCMPCs are able to remodel the matrix, as can be seen from the collagen staining in vitro, which is likely to affect matrix stiffness. In this respect, recently published data showed that intramyocardial administration of a stiffer matrix led to better functional recovery after acute MI [33]. Although the delivery route is different, it highlights the need for future investigation into the therapeutic effects of matrix stiffness for epicardial patches.…”

Section: Discussionmentioning

confidence: 94%

Epicardial application of cardiac progenitor cells in a 3D-printed gelatin/hyaluronic acid patch preserves cardiac function after myocardial infarction

et al. 2015

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The effect of matrix stiffness of injectable hydrogels on the preservation of cardiac function after a heart attack

Cited by 85 publications

References 40 publications

Effect of intra-myocardial Algisyl-LVR™ injectates on fibre structure in porcine heart failure

Effect of intra-myocardial Algisyl-LVR™ injectates on fibre structure in porcine heart failure

Modulation of Dental Pulp Stem Cell Odontogenesis in a Tunable PEG-Fibrinogen Hydrogel System

Epicardial application of cardiac progenitor cells in a 3D-printed gelatin/hyaluronic acid patch preserves cardiac function after myocardial infarction

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