Aim: To assess the effect of refined management in the operating room nursing on surgical efficiency and nursing satisfaction during laparoscopic radical resection of colon cancer. Methods: In this retrospective study, 100 patients with laparoscopic radical resection of colon cancer were enrolled into this study. There were 51 patients who received refined management (the observation group) and 49 patients who received routine nursing intervention (the control group). The effect of refined management in the operating room nursing was evaluated by comparing the surgical efficiency, quality of care ratings, pain scores, and the nursing satisfaction between the two groups. Results: The preoperative preparation time, surgical time, intraoperative bleeding volume, and time to first postoperative defecation in the observation group were all less than those in the control group after nursing intervention (all P<0.05). The observation group had higher scores than the control group in five categories: operating room environment and safety, drug and instrument management, hygiene and sterilization, nursing records, and nursing professionalism (all P<0.05). The numerical rating scale (NRS) pain scores of the patients in the observation group were lower than those of the control group at 12, 24, and 48 hours postoperatively (all P<0.05). The rate of satisfaction in the observation group was 96.1%. This was higher than the 91.8% in the control group (P<0.05). The multivariate regression analysis demonstrated that refined management intervention is an independent factor for patients' prognosis. Conclusion: The implementation of a refined management model in the operating room is effective in improving the quality of surgical care and surgical efficiency, and increasing patient satisfaction with nursing staff.