The effect of mechanical properties of iron oxide nanoparticle-loaded functional nano-carrier on tumor targeting and imaging

Choi, Won Il; Kim, Jayoung; Heo, Seon U.; Jeong, Yong Yeon; Kim, Young Ha; Tae, Giyoong

doi:10.1016/j.jconrel.2012.07.020

Cited by 27 publications

(8 citation statements)

References 41 publications

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“…Because of their unique size distribution, nanoscale carrier systems display size-dependent in vivo behaviors, including enhanced permeability and retention effect, controlled and ordered drug release, and the capacity of overcoming multiple biological barriers to achieving targeted drug delivery in vivo. In the past few decades, various multifunctional nanoparticles have been developed for tumor targeting, − imaging, − and treatment. , Nanoparticles achieve site-specific drug delivery through optimization of size and shape. Moreover, therapeutic compounds loaded in nanoparticles are expected to be released intracellularly to achieve efficacy, and as a result, in vitro release kinetics represents an important parameter scientists use to evaluate a nanomedicine.…”

Section: Introductionmentioning

confidence: 99%

Matrix Stiffness Differentially Regulates Cellular Uptake Behavior of Nanoparticles in Two Breast Cancer Cell Lines

Wang

Gong

Zhang

et al. 2017

ACS Appl. Mater. Interfaces

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Matrix stiffness regulates cell behavior in various biological contexts. In breast tumors, the deposition of extracellular matrix correlates with increasing matrix stiffness and poor survival. Nanoparticulate carriers represent a promising therapeutic vehicle for disease diagnosis and efficient anticancer drug delivery. However, how matrix stiffness influences cellular uptake of nanoparticles remains largely unexplored. Here, we selected photopolymerized polyacrylamide gels with varying stiffnesses as model substrates and studied the impact of matrix stiffness on cell morphology and nanoparticle uptake efficiency in two representative breast cancer cell lines with varying invasiveness, that is, MCF-7 with low invasiveness and MDA-MB-231 with high invasiveness. In our study, both cell lines showed similar morphological changes with changing stiffness. MCF-7 cells adhered on compliant substrates (1 kPa) showed a roundlike morphology with the lowest cell uptake efficiency among four stiffnesses under investigation at each given time point, whereas for MDA-MB-231 cells, the uptake efficiency showed no significant differences across varying stiffnesses. The percentages of MCF-7 cell proliferation on a 1 kPa substrate were significantly decreased at 48 and 72 h as compared to those on stiff substrates and coverslips. When treated with pluronic/d-α-tocopheryl polyethylene glycol 1000 succinate mixed micelle-loaded paclitaxel, cells on stiff substrates of 7, 20, and 25 kPa showed higher cell apoptosis rates as compared to those of cells on 1 kPa substrates. To sum up, our work presents an example of how physical cues impact specific cellular behavior and function, which may further contribute to engineering nanoparticulate delivery systems for more efficient delivery in vivo.

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Section: Introductionmentioning

confidence: 99%

Matrix Stiffness Differentially Regulates Cellular Uptake Behavior of Nanoparticles in Two Breast Cancer Cell Lines

Wang

Gong

Zhang

et al. 2017

ACS Appl. Mater. Interfaces

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“…In contrast, free Cy5.5 showed a minimum fluorescence compared to CaCO 3 AlgNC without tumor accumulation, indicating the fluorescence signal of the nanoparticle was not from the degraded free dye but the particle itself. The initially selective tumor targeting of CaCO 3 AlgNC might be associated with the EPR effect of the present nanosystem, ,, and the flexible state of the nanocarrier containing lots of small CaCO 3 particles instead of a single, large CaCO 3 might also help to reduce the nonspecific liver accumulation of the nanocarrier . Signals were also observed from the lung and liver, so some accumulation of the nanoparticle, probably slightly larger particles considering the particles are not monodispersed, in the lung, occurred.…”

Section: Resultsmentioning

confidence: 79%

Nanosized Ultrasound Enhanced-Contrast Agent for in Vivo Tumor Imaging via Intravenous Injection

Kim

Lee

Kim

et al. 2016

ACS Appl. Mater. Interfaces

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To enhance the detection limit of ultrasound (US) imaging, ultrasound enhanced-contrast agents (UECAs) that can go preferentially to the target tissue such as a tumor and amplify the US signal have been developed. However, nanosized UECAs among various UECAs developed are very limited to clearly demonstrate proper ability for selective tumor detection by US imaging upon their intravenous injection. In this study, we prepared CaCO3 nanoparticles that were formed inside a flexible and biocompatible pluronic-based nanocarrier. This nanosized UECA was stable in serum-containing media and generated CO2, more preferentially at low pH; thus, it could be detected by US imaging. After intravenous injection into tumor-bearing mice, this nanosized UECA showed a significant US contrast enhancement at the tumor site in 1 h, in contrast to no change in the liver, followed by a rapid clearance from the body in 24 h. Therefore, the present nanosized UECA could be applied as an effective diagnostic modality for in vivo tumor imaging by ultrasonography.

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“…Next, the medium was replaced with the fresh medium containing 10-time-diluted CCK-8 (cell counting kit-8, Dojindo Laboratories, Kumamoto, Japan), and the cells were further incubated for 1 h at 37 °C. The absorbance of the colored medium was measured at 450 nm by using a multiwell spectrophotometer 39 . All measurements were done in triplicate.…”

Section: Methodsmentioning

confidence: 99%

Bioinspired Heparin Nanosponge Prepared by Photo-crosslinking for Controlled Release of Growth Factors

Choi

Sahu

Vilos

et al. 2017

Sci Rep

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Growth factors have great therapeutic potential for various disease therapy and tissue engineering applications. However, their clinical efficacy is hampered by low bioavailability, rapid degradation in vivo and non-specific biodistribution. Nanoparticle based delivery systems are being evaluated to overcome these limitations. Herein, we have developed a thermosensitive heparin nanosponge (Hep-NS) by a one step photopolymerization reaction between diacrylated pluronic and thiolated heparin molecules. The amount of heparin in Hep-NS was precisely controlled by varying the heparin amount in the reaction feed. Hep-NS with varying amounts of heparin showed similar size and shape properties, though surface charge decreased with an increase in the amount of heparin conjugation. The anticoagulant activity of the Hep-NS decreased by 65% compared to free heparin, however the Hep-NS retained their growth factor binding ability. Four different growth factors, bFGF, VEGF, BMP-2, and HGF were successfully encapsulated into Hep-NS. In vitro studies showed sustained release of all the growth factors for almost 60 days and the rate of release was directly dependent on the amount of heparin in Hep-NS. The released growth factors retained their bioactivity as assessed by a cell proliferation assay. This heparin nanosponge is therefore a promising nanocarrier for the loading and controlled release of growth factors.

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The effect of mechanical properties of iron oxide nanoparticle-loaded functional nano-carrier on tumor targeting and imaging

Cited by 27 publications

References 41 publications

Matrix Stiffness Differentially Regulates Cellular Uptake Behavior of Nanoparticles in Two Breast Cancer Cell Lines

Matrix Stiffness Differentially Regulates Cellular Uptake Behavior of Nanoparticles in Two Breast Cancer Cell Lines

Nanosized Ultrasound Enhanced-Contrast Agent for in Vivo Tumor Imaging via Intravenous Injection

Bioinspired Heparin Nanosponge Prepared by Photo-crosslinking for Controlled Release of Growth Factors

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