The effect of medicaments used in endodontic regeneration on root fracture and microhardness of radicular dentine

Yassen, Ghaeth H.; Vail, Mychel M.; Chu, Tien Min G.; Platt, Jeffrey A.

doi:10.1111/iej.12046

Cited by 103 publications

(102 citation statements)

References 30 publications

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“…Thus, the indentations created using the reference point indenter on the collagen deficient surface dentine after exposure to Ca(OH) 2 might be harder to initiate, yet easier to propagate during cyclic stresses. Although the RPI technique does not allow separation of initiation and propagation of damage, a recent study found that Ca(OH) 2 caused a gradual decrease in root resistance to fracture and a gradual increase in microhardness of the same roots with time [26].…”

Section: Discussionmentioning

confidence: 99%

“…Exposure of the collagen matrix on the surfaces of the root canal dentine treated with TAP and DAP is illustrated in the SEM images ( Figs 2B-D). A recent in vitro study suggested that both TAP and DAP caused significant and continuous decrease in microhardness of radicular dentine after one and three months, respectively [26]. Additionally, TAP and DAP application for three months caused significant reduction in root resistance to fracture [26].…”

Section: Discussionmentioning

confidence: 99%

“…A recent in vitro study suggested that both TAP and DAP caused significant and continuous decrease in microhardness of radicular dentine after one and three months, respectively [26]. Additionally, TAP and DAP application for three months caused significant reduction in root resistance to fracture [26]. The ability of minocycline, one of the antibiotics in TAP, to chelate calcium and demineralize dental hard tissues [28,29] might explain the significantly higher indentation parameters reported among TAP-treated dentine compared to DAPtreated dentine.…”

Section: Discussionmentioning

confidence: 99%

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A novel approach to evaluate the effect of medicaments used in endodontic regeneration on root canal surface indentation

Yassen¹,

Chu²,

Gallant³

et al. 2013

Clin Oral Invest

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Objectives: To investigate the capability of a novel reference point indentation apparatus to test the indentation properties of root canal surface dentine treated with three intracanal medicaments used in endodontic regeneration. Materials and Methods:Immature human premolars were selected (n=22). Four specimens were obtained from each root and randomly assigned to three treatment groups and a control group. Each specimen was exposed to one of three treatment pastes (triple antibiotic (TAP), double antibiotic (DAP), or calcium hydroxide [Ca(OH) 2 ] or neutral de-ionized water (control) for one or four weeks. After each time-interval, the indentation properties of the root canal dentine surfaces were measured using a BioDent reference point indenter. Two-way ANOVA and Fisher's Protected Least Significant Differences were used for statistical analyses.Results: Significant differences in indentation parameters and estimated hardness between all groups at both time points were found. TAP treated dentine had the highest significant indentation parameters, followed by DAP treated dentine, untreated control dentine and Ca(OH) 2 treated dentine, respectively. Furthermore, TAP treated dentine had the lowest significant estimated hardness, followed by DAP treated dentine, untreated control dentine and Ca(OH) 2 treated dentine, respectively. Conclusion: BioDent reference point indenter was able to detect significant differences in indentation properties of root canal dentine treated with various medicaments.Clinical Relevance: The use of a reference point indenter is a promising approach to characterize the indentation properties of root canal surfaces without any surface modification. This might provide an in vitro mechanical measurement that is more representative of the actual clinical situation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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A novel approach to evaluate the effect of medicaments used in endodontic regeneration on root canal surface indentation

Yassen¹,

Chu²,

Gallant³

et al. 2013

Clin Oral Invest

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show abstract

“…This high concentration was suggested to have unfavorable effects on stem cells from apical papillae (14) and dental pulp stem cells (22), as well as on the chemical (23), physical (19), and mechanical properties of dentin (24). Therefore, the current study investigated the ability of a creamy consistency of low concentrations of TAP loaded into a methylcellulose system in maintaining the antibacterial properties of the original antibiotic mixture.…”

Section: Discussionmentioning

confidence: 99%

Direct and indirect antibacterial effects of various concentrations of triple antibiotic pastes loaded in a methylcellulose system

et al. 2016

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We investigated the direct and indirect(residual) antibacterial effects of various concentrations of triple antibiotic paste (TAP) loaded into a methylcellulose system. Enterococcus faecalis (E. faecalis) was grown on sterilized dentin blocks (n = 60) and treated with clinically used TAP (1,000 mg/ mL), low concentrations of methylcellulose-based TAP (100, 10, and 1 mg/mL), placebo paste, or 1.5% NaOCl (n = 10). The pastes were then removed, and biofilm disruption assays were performed. Additional dentin blocks (n = 120) were pretreated with the same experimental groups (n = 20). The pastes were rinsed off, and the samples were immersed independently in phosphate-buffered saline for 2 and 4 weeks (n = 10). E. faecalis was then grown on the dentin blocks, and biofilm disruption assays were performed. Fisher's Exact and Wilcoxon rank sum tests were used for statistical analyses. With regard to direct antibacterial effects, all treatment groups demonstrated complete eradication of biofilms in comparison to placebo paste, while 10 mg/mL of TAP or higher provided substantial residual antibacterial effects. However, dentin treated with 1 mg/mL of TAP or 1.5% NaOCl did not provide substantial residual antibacterial effects. Dentin pretreated with 10 mg/mL of TAP or higher exhibited extended residual antibacterial effects and can thus be used during endodontic regeneration. (J Oral Sci 58, 575-582, 2016)

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“…Therefore, recent studies have recommended the use of lower concentrations of these antibiotic medicaments ranging from 0.1-2 mg/mL to overcome their negative cytotoxic effects (12,14,15). The clinically applied concentration of various antibiotic medicaments was also found to negatively affect the mechanical (16,17) and chemical properties (18) of radicular dentine. A recent in vitro study reported a reduction in microhardness and root resistance to fracture after one and three months of application of intracanal medicaments, respectively (16).…”

Section: Introductionmentioning

confidence: 99%

Effects of two combinations of triple antibiotic paste used in endodontic regeneration on root microhardness and chemical structure of radicular dentine

et al. 2014

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We investigated the effects of tripleantibiotic paste (TAP) and modified triple antibiotic paste (MTAP) concentrations on the microhardness and chemical structure of radicular dentine. Human root cylinders were instrumented and randomized into four treatment groups and an untreated control group. Two treatment groups received 1 g/mL TAP or MTAP, and the other two treatment groups received 1 mg/mL methylcellulose-based TAP or MTAP. Cylinders were stored at 100% relative humidity for 4 weeks. Each root cylinder was subjected to a microhardness test before and after treatment. Different sets of radicular dentine specimens were treated as mentioned previously, and were examined using attenuated total reflection Fourier transform infrared spectroscopy. All treatment groups showed significant reductions in microhardness of roots when compared to untreated control roots at 1,000 and/or 500 µm from the pulp-dentine interface. However, 1 mg/mL methylcellulose-based antibiotics caused significantly less reduction in microhardness when compared to 1 g/mL antibiotics. In addition, 1 g/mL TAP and DAP caused significantly lower phosphate/amide I ratios when compared to other groups. The use of 1 mg/mL methylcellulose-based TAP and MTAP may minimize the reduction in microhardness of roots compared with the currently used 1 g/mL concentration of these antibiotics. (J Oral Sci 56, 245-251, 2014)

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The effect of medicaments used in endodontic regeneration on root fracture and microhardness of radicular dentine

Abstract: In this laboratory study, the 3-month application of triple antibiotic paste, double antibiotic paste or calcium hydroxide paste medicaments significantly reduced the root fracture resistance of extracted teeth compared to a 1-week application.

Cited by 103 publications

References 30 publications

A novel approach to evaluate the effect of medicaments used in endodontic regeneration on root canal surface indentation

A novel approach to evaluate the effect of medicaments used in endodontic regeneration on root canal surface indentation

Direct and indirect antibacterial effects of various concentrations of triple antibiotic pastes loaded in a methylcellulose system

Effects of two combinations of triple antibiotic paste used in endodontic regeneration on root microhardness and chemical structure of radicular dentine

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