The Effect of Melatonin Against Gastric Oxidative Stress and Dyslipidemia in Streptozotocin-Induced Diabetic Rats

Abstract

Objective. The aim of this study was to evaluate the possible protective effects of MT against gastric oxidative stress and dyslipidemia in streptozotocin (STZ)-induced diabetic rats. Methods. Forty male Wistar rats were randomly divided into five groups: control, diabetic, MT 5 mg/kg+ STZ, MT 10 mg/kg+ STZ and MT 20 mg/kg+ STZ. STZ (60 mg/kg) was intraperitoneally (ip) injected as a single dose for diabetes induction. One week after STZ administration, MT was injected daily as ip for 14 days. The levels of malondialdehyde (MDA), total thiol and glutathione, as well as superoxide dismutase (SOD) and catalase activities were measured in gastric tissue. Serum concentrations of triglycerides (TG), total cholesterol (TC), high density lipoprotein (HDL) and low density lipoprotein (LDL) were also determined. Results. Serum glucose significantly increased in diabetic group compared to control group. STZ induced a significant decrease in gastric tissue levels of total thiol, glutathione, catalase and SOD activities and a significant increase in MDA concentration. In diabetic rats, serum TG, LDL and TC were significantly higher and HDL was significantly lower than in the control group. Treatment of diabetic rats with MT caused a significant increase in gastric total thiol content and glutathione concentration as well as SOD and catalase activities. Gastric MDA concentration and serum LDL, TG and TC were significantly lower in MTtreated groups when compared with diabetic group. Conclusion. These data suggested that MT has a therapeutic effect on gastric oxidative damage and dyslipidemia induced by diabetes that possibly may be due to its antioxidant effects.

Melatonin protects the heart and pancreas by improving glucose homeostasis, oxidative stress, inflammation and apoptosis in T2DM-induced rats

“…In the current study, the administration of MLT to diabetic rats caused a significant improvement in the lipid profile compared with the STZ-induced diabetic rats. These findings agree with those in which MLT improved the serum lipid fractions in serum of diabetic rats [ 43 , 44 , 45 ]. The mechanism underlying the lowering effect of MLT on cholesterol might be through decreasing the absorption of cholesterol from the intestine or increasing endogenous cholesterol clearance [ 46 ].…”

Melatonin Supplementation Attenuates the Pro-Inflammatory Adipokines Expression in Visceral Fat from Obese Mice Induced by A High-Fat Diet

“…Wistar rats induced to diabetes by streptozotocin and treated with melatonin (10 mg/kg and 20 mg/kg) i.p. for two weeks also showed significant reductions in TG, TC and LDL-cholesterol levels [45]. However, in another study [46], C57Bl/6 mice induced to obesity by an HFD and treated with melatonin (10 mg/kg) for 12 weeks presented a reduction in body mass and LDL levels but not in TG levels.…”

Resveratrol Plays a Protective Role against Premature Ovarian Failure and Prompts Female Germline Stem Cell Survival

“…Here, the activities of the antioxidative enzymes glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) and the contents of the small molecules glutathione (GSH), ROS, and malondialdehyde (MDA) were used to assess oxidative stress levels in tissues or cells [31,32,33]. TNF-α, interleukin (IL)-6, and IL-10 were used to evaluate the degree of inflammation.…”