The effect of melatonin on the liver of rats exposed to microwave radiation

Abstract

Abstract:Objectives: We aimed to clarify if melatonin treatment (2 mg/kg i.p.) may favorably impact the liver tissue in rats exposed to microwave radiation. The experiment was performed on 84 six-weeks-old Wistar male rats exposed for 4h a day, for 20, 40 and 60 days, respectively, to microwaves (900 MHz, 100-300 microT, 54-160 V/m). Rats were divided in to four groups: I (control) -rats treated with saline, II (Mel) -rats treated with melatonin, III (MWs) -microwave exposed rats, IV (MWs + Mel) -MWs exposed rats treated with melatonin. We evaluated oxidative stress parameters (malondialdehyde and carbonyl group content), catalase, xanthine oxidase, deoxyribonuclease I and II activity. Background: Oxidative stress is the key mechanism of the microwave induced tissue injury. Melatonin, a lipophilic indoleamine primarily synthesized and released from the pineal gland is a powerful antioxidant. Results: Exposure to microwaves caused an increase in malondialdehyde after 40 (p < 0.01), protein carbonyl content after 20 (p < 0.05), catalase (p < 0.05) and xantine oxidase activity (p < 0.05) after 40 days. Increase in deoxyribonuclease I activity was observed after 60 days (p < 0.05), while deoxyribonuclease II activity was unaffected. Melatonin treatment led to malondialdehyde decrease after 40 days (p< 0.05), but surprisingly had no effect on other analyzed parameters. Conclusion: Melatonin exerts certain antioxidant effects in the liver of rats exposed to microwaves, by diminishing the intensity of lipid peroxidation (Fig. 6, Ref. 32).Text in PDF www.elis.sk.

Effect of Apocynin on Liver Toxicity Induced by Microwaves in Rats

“…In contrast with the current results, Djordjevic et al [32] failed to demonstrate the significant change in MDA level in animals exposed to MWs for 20 days. However, they demonstrated that the increase of MDA occurred in liver tissue of rats exposed to microwaves after prolonged exposition period (40 and 60 days).…”

“…During the last few decades, there has been an increase in the number of devices that emit microwaves. Such devices are used predominantly in telecommunications, but also in other society sectors and in households [1] . Nowadays, we are facing growing public concerns regarding the potential hazard to man from exposure to microwaves.…”

Antioxidative, membrane protective and antiapoptotic effects of melatonin, in silico study of physico-chemical profile and efficiency of nanoliposome delivery compared to betaine

“…Obtained results are in accordance with those in our previous study about the protective effect of melatonin on rats exposed to microwave irradiation. [13][14][15] In the present study, it was shown that exposure to betaine in Fasmediated apoptotic conditions may induce an increase in DNase activity in treated hepatocytes. This result is in accordance with known literature data which claim that betaine was proven to considerably destabilize DNA.…”

“…11,12 Our previous results pointed out melatonin as protector against microwave irradiation. [13][14][15] Like an electron donor, melatonin has been shown to increase the efficiency of the electron transport chain and, as a consequence, to reduce electron leakage and the generation of free-radicals. 16 Beside direct free-radical scavenging properties, it also has been shown to have indirect antioxidative effects, through the upregulation of several antioxidative enzymes.…”

Melatonin treatment prevents carbon tetrachloride-induced acute lung injury in rats by mitigating tissue antioxidant capacity and inflammatory response

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