The Effect of Melissa officinalis Extract on Streptozotocin-Induced Diabetes in Rats: A Stereological Study on Pancreatic Islets and Beta-cells

Ashkani-Esfahani, Soheil; Ebrahimi, Alireza; Bahmani-Jahromi, Maryam; Nadimi, Elham; Arabzadeh, Hossein; Jalalpour, Mohammad Hassan; Ghasemnezhad, Ali; Ebrahimi, Sedigheh

doi:10.30699/jambs.29.132.34

Cited by 3 publications

(2 citation statements)

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“…Streptozotocin (STZ) is a toxic agent that disrupts the function of insulin-producing beta cells, and it has been used in previous studies to induce DM in animal models (18)(19)(20)(21). After an overnight fasting period, rats were injected intraperitoneally with a single dosage of 60 mg/kg body weight of STZ (Sigma-Aldrich®, Steinhelm, Germany).…”

Section: Streptozotocin (Stz) Administration and Detection Of Hyperglycemiamentioning

confidence: 99%

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Salvia Officinalis Protects Pancreatic Beta-cells Against Streptozotocin-Induced Damage; A Stereological Study

Ashkani-Esfahani

Noorafshan

Ebrahimi

et al. 2021

Jundishapur J Nat Pharm Prod

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Background: Diabetes mellitus (DM) is a chronic disease, progressing due to inadequate secretion of insulin by pancreas. Salvia officinalis (SVO) has anti-inflammatory and anti-oxidative potentials, which may be beneficial in regulating underlying causes of DM. Objectives: In this study, we aimed to estimate the protective effects of SVO against Streptozotocin (STZ)-induced pancreatic injury in rat models of DM. Methods: Forty-eight male Sprague-Dawley rats were randomly divided into four groups (n = 12); C1: normal group with no treatment, C2: diabetic group with no treatment, E1: diabetic group treated with 200 mg/kg of the SVO extract, and E2: diabetic group treated with 400 mg/kg of the SVO extract. All groups received a single dose of STZ on day 7 except C1. Pancreas volume, shrinkage, volume densities of the islets, numerical densities, and volume of the beta cells were measured using stereological methods. Results: Blood sugar (BS) levels were significantly lower in SVO-treated groups comparing to C2 group. Also, volume densities and total number of islets and beta cells in E1 and E2 groups were higher than C2 (P < 0.05), but lower than C1 (P < 0.05). Volume densities of the islets and beta cells, and total number of beta cells in E1, and volume densities of the islets and beta cells in E2 groups were considerably higher than C2 group (P < 0.05). Conclusions: Our result showed the beneficial effects of SVO extract regarding pancreatic damage. We concluded that SVO might be prescribed as a therapeutic food supplement for patients with diabetes.

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Section: Streptozotocin (Stz) Administration and Detection Of Hyperglycemiamentioning

confidence: 99%

“…The blood sugar (BS) levels of the rats were tested by glucometer (Rightest GM110, Bionime™; Switzerland) every day during the injection of STZ (day 7 to day 14). Animal models, which showed a BS level of ≥ 300 mg/dL were labeled as diabetic (20,21).…”

Section: Streptozotocin (Stz) Administration and Detection Of Hyperglycemiamentioning

confidence: 99%