The effect of membrane-strain rate and of temperature on erythrocyte fragility and critical hemolytic volume

Seeman, Philip; Sauks, T.; Argent, W.; Kwant, W.O.

doi:10.1016/0005-2736(69)90162-x

Cited by 96 publications

(54 citation statements)

References 23 publications

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“…In our continuous dilution method we find, hke other authors [31,32], a shift of the osmotic fragility curve to the hypotonic side, when compared to the classical discontinuous method. Control experiments showed that incubation up to 21 h under the conditions used for the PC replacement gives only a slight shift in osmotic fragility of the cells (Fig.…”

Section: Effect On Osmotic Fragility and Membrane Permeability For Pomentioning

confidence: 48%

“…Cells, sampled from the incubation mixture, were washed twice with buffer at 25-35°C. The osmotic fragility was determined by measuring the change in turbidity of an erythrocyte suspension at 690 nm as function of salt concentration [31][32][33]. 10 /~1 of packed cells were suspended in 10 ml of isotonic buffer in a thermostated cuvette.…”

Section: Preparation Of the Transfer Proteinmentioning

confidence: 99%

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The membrane of intact human erythrocytes tolerates only limited changes in the fatty acid composition of its phosphatidylcholine

Kuypers

Roelofsen

Kamp

et al. 1984

Biochimica et Biophysica Acta (BBA) - Biomembranes

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(1) Using the phosphatidylcholine specific transfer protein from bovine liver, native phosphatidylcholine from intact human erythrocytes was replaced by a variety of different phosphatidylcholine species without altering the original phospholipid and cholesterol content. (2) The replacement of native phospharidylcholine by the disaturated species, 1,2-dipalmitoyl-and 1,2-distearoylphosphatidyicholine, proceeded at a low rate and extensive replacement could only be achieved by repeatedly adding fresh donor vesicles. The replacement by disaturated molecules was accompanied by a gradual increase in osmotic fragility of the cells, finally resulting in hemolysis when 40% of the native PC had been replaced. Up to this lyric concentration, the replacement did not affect the permeability of the membrane for potassium ions. (3) Essentially, all of the PC in the outer monolayer of the membrane could be replaced by 1-palmitoyl-2-oleoyl-and 1-palmitoyl-2-1ino-leoylphosphatidylcholine. These replacements did not alter the osmotic fragility of the cells, nor the K + permeability of the membrane. (4) Increasing the total degree of unsaturation of the phosphatidyicholine species modified the properties of the membrane considerably. Replacement by 1,2-dilinoleoylphosphatidylcholine resulted in a progressive increase in osmotic fragility and hemolysis started to occur after 30% of the native PC had been replaced by this species. K ÷ permeability was found to be slightly increased in this case. Cells became leaky for K ÷ upon the introduction of 1-palmitoyl-2-arachidonoylphosphatidyicholine in the membrane. The increased permeability was also reflected by an apparent increase in the resistance of the cells against osmotic shock. (5) The conclusions to be drawn are that (i) 1-palmitoyl-2-oleoyi-and l-paimitoyi-2-1inoleoylphosphatidyicholine are species which fit most optimally into the erythrocyte membrane; (ii) loss of membrane stability results from an increase in the degree of saturation of phosphatidyicholine (unsaturation index > 0.5) and (iii) the permeability is enhanced by increasing the content of highly unsaturated species (unsaturation index > 1.0).

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Section: Effect On Osmotic Fragility and Membrane Permeability For Pomentioning

confidence: 48%

Section: Preparation Of the Transfer Proteinmentioning

confidence: 99%

The membrane of intact human erythrocytes tolerates only limited changes in the fatty acid composition of its phosphatidylcholine

Kuypers

Roelofsen

Kamp

et al. 1984

Biochimica et Biophysica Acta (BBA) - Biomembranes

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“…Seeman et al 26 have found that potassium ions escape from sublethally damaged erythrocytes long before hemoglobin release occurs. Indeed, flow rates of 120 ml/min in our trials in vitro resulted in a marked increase in potassium concentration of 5.58 ± 1.04 mmol/liter.…”

Section: Discussionmentioning

confidence: 99%

Autologous blood perfusion for myocardial protection during coronary angioplasty: a feasibility study.

Lehmann¹,

Atwood²,

Snyder³

et al. 1987

Circulation

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lactate dehydrogenase, and potassium remained constant before and after the perfusion and the control inflations. The maximum pain score was significantly lower with the perfusion inflation (4.1 ± 0.8 vs 6.0 ± 0.9, p<.003). Relative to baseline, the maximum ST segment elevation during the perfusion inflation (0.5 ± 0.3 mm) was nearly one-fourth that during the control inflation (1.9 ± 0.6 mm, p<.02). Thus, myocardial protection with oxygenated autologous blood perfusion at rates of 60 ml/min appears to be a safe and effective technique that may permit increased inflation time and extend the range of coronary angioplasty to include individuals at high risk for the procedure.

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“…17; Seeman, Sauks, Argent and Kwant, 1969;Livne and Raz, 1971), similar heterogeneous conditions are obtained, but improved control is possible over the gross rate of change of the external solute concentration.…”

Section: Osmotic Hemolysis Systemsmentioning

confidence: 87%

“…An increase in the amount of K+ lost by cells in slow versus fast osmotic hemolysis has been observed by Seeman, Sauks, Argent and Kwant (1969) and by Livne and Raz (1971). Both groups attribute the delay in Hb loss in slow osmotic hemolysis to the excess K+ loss.…”

Section: Summary: a Single Population Distribution In Osmotic Responsementioning

confidence: 93%

Molecular sieving action of the cell membrane during gradual osmotic hemolysis

MacGregor¹

1977

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The effect of membrane-strain rate and of temperature on erythrocyte fragility and critical hemolytic volume

Cited by 96 publications

References 23 publications

The membrane of intact human erythrocytes tolerates only limited changes in the fatty acid composition of its phosphatidylcholine

The membrane of intact human erythrocytes tolerates only limited changes in the fatty acid composition of its phosphatidylcholine

Autologous blood perfusion for myocardial protection during coronary angioplasty: a feasibility study.

Molecular sieving action of the cell membrane during gradual osmotic hemolysis

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