The Effect of Methanolic Valeriana officinalis Root Extract on Adipocyte Differentiation and Adiponectin Production in 3T3-L1 Adipocytes

Harada, Kousuke; Kato, Yuria; Takahashi, J.; Imamura, Hiyoko; Nakamura, Natsume; Nishina, Atsuyoshi; Phay, Nyunt; Miki, Tsuneharu; Shimizu, Makoto; Kobayashi‐Hattori, Kazuo

doi:10.1007/s11130-019-00790-2

Cited by 8 publications

(10 citation statements)

References 23 publications

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“…Four of these studies used chemical in vitro assays to measure antioxidant capacity and acetylcholinesterase inhibitory activity (Pilerood & Prakash, 2014;Li et al, 2015;Chen et al, 2016;Katsarova et al, 2018). In the other two in vitro studies, cell lines have been employed, particularly, the human neuroblastoma SH-SY5Y cells and the mouse 3T3-L1 preadipocytes (Gonulalan et al, 2018;Harada et al, 2020). Antioxidants are compounds that prevent free radical harmful effects by scavenging and metal chelating.…”

Section: Resultsmentioning

confidence: 99%

“…Finally, the methanolic extract of Valeriana officinalis root demonstrated to be beneficial against type 2 diabetes by promoting dose-dependently 3T3-L1 adipocytes differentiation; this is related to direct binding to peroxisome proliferator-activated receptor γ (PPARγ) (Harada et al, 2020). Table 2 summarizes the main results for the fourteen in vivo studies.…”

Section: In Vitro Studiesmentioning

confidence: 99%

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Updating the biological interest of 'Valeriana officinalis'

Sánchez¹,

González-Burgos²,

Iglesias³

et al. 2021

Mediterr. Bot.

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Valeriana officinalis L. (Caprifoliaceae) has been traditionally used to treat mild nervous tension and sleep problems. The basis of these activities are mainly attributed to valerenic acid through the modulation of the GABA receptor. Moreover, V. officinalis is claimed to have other biological activities such as cardiovascular benefits, anticancer, antimicrobial and spasmolytic. The current review aims to update the biological and pharmacological studies (in vitro, in vivo and clinical trials) of V. officinalis and its major secondary metabolites in order to guide future research. Databases PubMed, Science Direct and Scopus were used for literature search including original papers written in English and published between 2014 and 2020. There have been identified 33 articles which met inclusion criteria. Most of these works were performed with V. officinalis extracts and only a few papers (in vitro and in vivo studies) evaluated the activity of isolated compounds (valerenic acid and volvalerenal acid K). In vitro studies focused on studying antioxidant and neuroprotective activity. In vivo studies and clinical trials mainly investigated activities on the nervous system (anticonvulsant activity, antidepressant, cognitive problems, anxiety and sleep disorders). Just few studies were focused on other different activities, highlight effects on symptoms of premenstrual and postmenopausal syndromes. Valeriana officinalis continues to be one of the medicinal plants most used by today's society for its therapeutic properties and whose biological and pharmacological activities continue to arouse great scientific interest as evidenced in recent publications. This review shows scientific evidence on traditional uses of V. officinalis on nervous system.

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Section: Resultsmentioning

confidence: 99%

Section: In Vitro Studiesmentioning

confidence: 99%

Updating the biological interest of 'Valeriana officinalis'

Sánchez¹,

González-Burgos²,

Iglesias³

et al. 2021

Mediterr. Bot.

View full text Add to dashboard Cite

show abstract

“…Cells at a concentration of 5.0 × 10 3 cells/mL in 24-well plates were cultured at 37 °C in a humidified atmosphere of 5% CO 2 , with a pre-adipocyte maintenance medium containing Dulbecco’s modified Eagle’s medium, DMEM (4.5 g/L glucose) (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) supplemented with 10% bovine serum (Thermo Fisher Scientific Inc., Waltham, MA) and 1% penicillin–streptomycin (Thermo Fisher Scientific Inc). Two days post-confluence formation, the medium was replaced with an adipocyte differentiation medium and cultured separately with or without VA (Extrasynthese S.A., Genay, France) for 8 days as previously described . Rosiglitazone (rosi) (FUJIFILM Wako Pure Chemical Corporation) at a final concentration of 100 nM was added to the adipocyte differentiation medium during the first 2 days as a positive control.…”

Section: Methodsmentioning

confidence: 99%

“…21 Recently, we have demonstrated that methanolic Valeriana officinalis root extract could promote adipocyte differentiation and adiponectin production in 3T3-L1 adipocytes and identified valerenic acid (VA) as one of the likely active components. 22 Although VA has been reported to have anxiolytic activity 23 and central nervous depressant activity, 24 its potential effects on adipocytes are yet to be determined.…”

Section: Introductionmentioning

confidence: 99%

Valerenic Acid Promotes Adipocyte Differentiation, Adiponectin Production, and Glucose Uptake via Its PPARγ Ligand Activity

Takahashi

Miki

et al. 2022

ACS Omega

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Although valerenic acid (VA) is an important marker compound for quantitative assessment of Valeriana officinalis products, little is known about its potential effects on adipocytes. We investigated the effects of VA on adipocyte differentiation, adiponectin production, and glucose uptake using 3T3-L1 adipocytes. The results showed that VA promoted adipocyte differentiation and increased the gene expression of adipogenesis and glucose uptake-related proteins, including peroxisome proliferator-activated receptor gamma (PPARγ), cytosine-cytosine-adenosine-adenosine-thymidine enhancer binding protein alpha (C/EBPα), adiponectin, and glucose transporter 4 (GLUT4). Additionally, cell cultures treated with VA had elevated adiponectin secretion and glucose uptake. The PPARγ luciferase assay indicated VA as a partial agonist of PPARγ, while the analysis using its antagonist, GW9662, and a docking simulation between PPARγ and VA revealed the binding site of VA as likely adjacent to the Ω loop pocket of PPARγ. Taken together, these results demonstrate that VA acts as a PPARγ partial agonist to promote adipocyte differentiation, adiponectin production, and glucose uptake.

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“…For example, compounds such as purpurin [ 39 ], bergamottin [ 40 ] and quercetin [ 41 ] were shown to inhibit adipogenic differentiation in 3T3-L1 cells mainly through downregulating the expression of two adipogenic factors, CCAAT enhancer-binding protein alpha (C/EBPα) and peroxisome proliferator activated receptor-gamma (PPARγ). On the other hand, several obesogenic compounds, such as the methanolic Valerian root extract [ 42 ], oleic acid [ 43 ] and vulpinic acid [ 44 ], have also been studied, in order to dissect the mechanisms of action during the differentiation process of 3T3-L1 cells. Furthermore, the biological role of several miRNAs, such as miR-152, in the process of preadipocyte proliferation and differentiation, has also been studied by using 3T3-L1 cells [ 45 ].…”

Section: Modeling Adipogenesis Via Two-dimensional (2d) Modelsmentioning

confidence: 99%

Modeling Adipogenesis: Current and Future Perspective

Bahmad

Daouk

Azar

et al. 2020

Cells

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Adipose tissue is contemplated as a dynamic organ that plays key roles in the human body. Adipogenesis is the process by which adipocytes develop from adipose-derived stem cells to form the adipose tissue. Adipose-derived stem cells’ differentiation serves well beyond the simple goal of producing new adipocytes. Indeed, with the current immense biotechnological advances, the most critical role of adipose-derived stem cells remains their tremendous potential in the field of regenerative medicine. This review focuses on examining the physiological importance of adipogenesis, the current approaches that are employed to model this tightly controlled phenomenon, and the crucial role of adipogenesis in elucidating the pathophysiology and potential treatment modalities of human diseases. The future of adipogenesis is centered around its crucial role in regenerative and personalized medicine.

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The Effect of Methanolic Valeriana officinalis Root Extract on Adipocyte Differentiation and Adiponectin Production in 3T3-L1 Adipocytes

Cited by 8 publications

References 23 publications

Updating the biological interest of 'Valeriana officinalis'

Updating the biological interest of 'Valeriana officinalis'

Valerenic Acid Promotes Adipocyte Differentiation, Adiponectin Production, and Glucose Uptake via Its PPARγ Ligand Activity

Modeling Adipogenesis: Current and Future Perspective

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