The effect of methyltestosterone (MT) on sex differentiation and growth in juvenile yellow perch (Perca flavescens)

Othman, Razib M.; Ron, Xiao-Jun; Yao, Hong; O’Bryant, Paul; Rapp, Dean; Pei, J C; Wu, Hongjuan; Wang, Hanping

doi:10.1007/s10695-021-01038-0

Cited by 9 publications

(7 citation statements)

References 30 publications

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“…Gonadal development and sexual activity are not only determined by the interactions of multiple genes but also by environmental factors, such as temperature or hormone exposure [ 46 , 47 ], and exposure to exogenous hormones during gonadal differentiation can alter the population gender ratio [ 48 , 49 ]. Steroid hormones are natural and induce sex change processes [ 50 ], among which E2 is one of the most potent estrogens impacting sex differentiation [ 51 ] and MT is one of the most potent androgens impacting sex differentiation [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

Expression and Characterization of the Spats1 Gene and Its Response to E2/MT Treatment in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Luo

Zhu

Chen

et al. 2022

Animals

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Spats1 (spermatogenesis-associated, serinerich 1) has been characterized as a male-biased gene which acts an important role in the germ cell differentiation of mammals. Nevertheless, the function of Spats1 in the Chinese soft-shelled turtle (P. sinensis) has not yet been reported. To initially explore the expression of Spats1 in P. sinensis and its response to sex steroid treatment, we cloned the CDS of Spats1 for the first time and analyzed its expression profile in different tissues, including the testes in different seasons. The Spats1 cDNA fragment is 1201 base pairs (bp) in length and contains an open reading frame (ORF) of 849 bp, which codes for 283 amino acids. Spats1 mRNA was highly expressed in the testes (p < 0.01) and barely detectable in other tissues. In P. sinensis, the relative expression of Spats1 also responsive to seasonal changes in testis development. In summer (July) and autumn (October), Spats1 gene expression was significantly higher in the testes than in other seasons (p < 0.05). Spats1 mRNA was found to be specifically expressed in germ cells by chemical in situ hybridization (CISH), and it was mainly located in primary spermatocytes (Sc1), secondary spermatocytes (Sc2) and spermatozoa (St). Spats1 expression in embryos was not significantly changed after 17α-methyltestosterone (MT)and 17β-estradiol (E2) treatment. In adults, MT significantly induced Spats1 expression in male P. sinensis. However, the expression of Spats1 in testes was not responsive to E2 treatment. In addition, the expression of Spats1 in females was not affected by either MT or E2 treatment. These results imply that Spats1 is a male-specific expressed gene that is mainly regulated by MT and is closely linked to spermatogenesis and release in P. sinensis.

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Section: Discussionmentioning

confidence: 99%

Expression and Characterization of the Spats1 Gene and Its Response to E2/MT Treatment in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Luo

Zhu

Chen

et al. 2022

Animals

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“…The serum 17-MT levels obtained may have been just above the upper limit for growth promotion. In contrast, Othman et al ( 2022) [33] reported the highest growth performance parameters such as the highest body weight gain, and SGR after the feeding of 50 mg/kg MT-treated feed at 38 and 46 days. Bautista et al ( 2022) [34] also recently examined the weight gain and feed acceptance by the fish were higher in male catfish fed diets containing 60 and 90 mg•kg-17-MT.…”

Section: Growth Performancementioning

confidence: 90%

“…The 17-MT treated feed deviated from the anticipated typical male: a female population at 38 and 46 dph (1:1). The 50 mg/kg 17-MT dosage feed groups fed with 17-MT at 67 dph produced fish that were 37% and 25% intersex, respectively [33]. The fish's sex ratio did not change across treatment groups; however Chitosan nanoparticles increased the effectiveness of LET.…”

Section: Sex Ratio and Histologymentioning

confidence: 92%

Effects of dietary aromatase inhibitors on masculinization of rosy barb (Pethia conchonius): Evidence from growth, coloration and gonado-physiological changes

Lal,

Biswas,

Singh

et al. 2023

PLoS ONE

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The objective of this study was to reveal the growth, colouration and gonado-physiological changes due to the exogenous aromatase inhibitor (AIs) in an ornamental fish. 17α-methyltestosterone (MT) and letrozole (LET) were used as potential AIs. The AI were supplemented with a gel-based feed (LET: 50, 100, 150 and MT: 12.5, 25, 37.5 mg/kg feed) in Rosy barb, Pethia conchonius fry. The fishes were reared in a 45-L glass tank using AI treated gel-based feed for 3 months. Growth in AI-based diets was reduced but the reduction was minimal compared to the control. At 25 mg/kg feed of 17 MT, the highest male proportion (84.72% 6.05%) was recorded, which was significantly higher (P≤0.05) than other groups. L*, a*, and b* values showed that 17α-MT-fed groups had brighter coloration (P≤0.05). Histological sections showed that LET-17α-MT suppressed ovarian development, causing atretic oocytes. Testicular development was unaffected. 25 mg/kg-treated feed increased SOD, CAT, GST, and GPX. The AI (MT) at 25 mg/kg gel-based feed could therefore be utilised for musculinization without impacting growth, colour, and antioxidant activity of rosy barb, which serves the entire male population in the ornamental fish sector.

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“…The MT feeding was administered from 20 to 68 dpb stage, and after that, the feeding of the fish continued on the control diet without the hormone. Three breedingenvironment replicates of each treatment group were set up, and MT concentrations were based on previous studies [16][17][18]. After the 140 dpb stage period, body length, total length, and weight of each individual were measured, and fin, whole-body tissue, and gonadal tissue samples were collected for subsequent hormone-level analysis histological, sex determination, and transcriptomic analyses.…”

Section: Experimental Design and Steroid Treatmentmentioning

confidence: 99%

Molecular and Physiological Effects of 17α-methyltestosterone on Sex Differentiation of Black Rockfish, Sebastes schlegelii

Huang,

Liu,

Wang

et al. 2024

Genes

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It is widely known that all-female fish production holds economic value for aquaculture. Sebastes schlegelii, a preeminent economic species, exhibits a sex dimorphism, with females surpassing males in growth. In this regard, achieving all-female black rockfish production could significantly enhance breeding profitability. In this study, we utilized the widely used male sex-regulating hormone, 17α-methyltestosterone (MT) at three different concentrations (20, 40, and 60 ppm), to produce pseudomales of S. schlegelii for subsequent all-female offspring breeding. Long-term MT administration severely inhibits the growth of S. schlegelii, while short term had no significant impact. Histological analysis confirmed sex reversal at all MT concentrations; however, both medium and higher MT concentrations impaired testis development. MT also influenced sex steroid hormone levels in pseudomales, suppressing E2 while increasing T and 11-KT levels. In addition, a transcriptome analysis revealed that MT down-regulated ovarian-related genes (cyp19a1a and foxl2) while up-regulating male-related genes (amh) in pseudomales. Furthermore, MT modulated the TGF-β signaling and steroid hormone biosynthesis pathways, indicating its crucial role in S. schlegelii sex differentiation. Therefore, the current study provides a method for achieving sexual reversal using MT in S. schlegelii and offers an initial insight into the underlying mechanism of sexual reversal in this species.

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The effect of methyltestosterone (MT) on sex differentiation and growth in juvenile yellow perch (Perca flavescens)

Cited by 9 publications

References 30 publications

Expression and Characterization of the Spats1 Gene and Its Response to E2/MT Treatment in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Expression and Characterization of the Spats1 Gene and Its Response to E2/MT Treatment in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Effects of dietary aromatase inhibitors on masculinization of rosy barb (Pethia conchonius): Evidence from growth, coloration and gonado-physiological changes

Molecular and Physiological Effects of 17α-methyltestosterone on Sex Differentiation of Black Rockfish, Sebastes schlegelii

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