The effect of milk and casein proteins on the adherence of Streptococcus mutans to saliva-coated hydroxyapatite

Vacca-Smith, A.M.; Wuyckhuyse, B.C. Van; Tabak, Lawrence A.; Bowen, Willis H.

doi:10.1016/0003-9969(94)90059-0

Cited by 92 publications

(57 citation statements)

References 35 publications

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“…This component of milk has previously been shown to be a potent inhibitor of adhesion of S. mutans (strain MT8148) to S-HA beads, and the findings of the present study are consistent with the work of Oho and co-workers [25]. It is well known that consumption of milk can be protective against occurrence of dental caries, and a study by Vacca-Smith et al [26] reported that treatment of S-HA beads with milk caused a reduction in the adherence of S. mutans GS-5. In the same study, lactose was shown to have no function in the anti-adhesive properties of milk, which has also been observed in our laboratory (data not shown).…”

Section: Assessment Of Anti-adhesive Effect Of Milk Componentssupporting

confidence: 93%

Inhibition of adhesion of Streptococcus mutans to hydroxylapatite by commercial dairy powders and individual milk proteins

Halpin

O'Connor

McMahon

et al. 2008

Eur Food Res Technol

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The aim of this study was to investigate the inhibitory effect of various dairy powders and milk constituents on the adhesion of a clinical isolate of Streptococcus mutans to hydroxylapatite (HA), an analogue of tooth enamel. Adhesion of a microorganism to a cell surface such as epithelial cells or tooth enamel is considered to be the first step in pathogenesis. Inhibiting this process may have therapeutic effects in vivo. The adherence assays were performed by incubating S. mutans with HA in the presence of each test material for 45 min, followed by centrifugal separation of the HA. Unbound bacteria were then quantified using a fluorescent dye. Sweet and Acid WPC80, buttermilk powder and cream powder were found to very effectively inhibit adherence of S. mutans to phosphate-buffered saline coated HA (PBS-HA). Sodium caseinate and the casein fractions a-,b-and j-casein were also found to show high levels of anti-adhesive activity. A selection of test materials were assessed using saliva-coated HA (S-HA), and similar trends were observed. The results suggest commercial dairy powders, and certain milk proteins, can inhibit adhesion of S. mutans to HA and may have potential to control dental caries.

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Section: Assessment Of Anti-adhesive Effect Of Milk Componentssupporting

confidence: 93%

Inhibition of adhesion of Streptococcus mutans to hydroxylapatite by commercial dairy powders and individual milk proteins

Halpin

O'Connor

McMahon

et al. 2008

Eur Food Res Technol

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“…Other reports also demonstrate that purified S-IgA in human milk can inhibit the adhesion of streptococci mutans to saliva-coated HA disks [33,34] . Although several papers have reported that S-IgA may contribute to the formation of biofilms and boost the adherence of bacteria [35] , it is important to note that all of the S-IgA used in these previous studies focused on the original S-IgA in the host's saliva or serum, while the S-IgA investigated in our study was induced by the targeted anticaries DNA plasmid pGJA-P/VAX1.…”

Section: Discussionmentioning

confidence: 99%

Anti-caries DNA vaccine-induced secretory immunoglobulin A antibodies inhibit formation of Streptococcus mutans biofilms in vitro

Huang

Liu

et al. 2012

Acta Pharmacol Sin

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Aim: To investigate the effects of anti-caries DNA vaccine-induced salivary secretory immunoglobulin A (S-IgA) antibodies on Streptococcus mutans (S. mutans) adherence and biofilms formation in vitro. Methods: Adult female Wistar rats were intranasally immunized with the anti-caries DNA vaccine pGJA-P/VAX. Their saliva samples were collected at different times after the immunization, and S-IgA antibody level in the saliva and its inhibition on S. mutans adherence were examined. The effects of S-IgA in the saliva with the strongest inhibitory effects were examined at 3 different stages, ie acquired pellicles, biofilm formation and production of mature biofilms. The number of viable bacteria and depth of the biofilm at 16 h in each stage were determined using counting colony forming units and using a confocal laser scanning microscopy (CLSM). The participation of S-IgA in acquired pellicles and its aggregation with S. mutans were also observed under CLSM. Results: The S-IgA titer in saliva reached its peak and exhibited the strongest inhibition on S. mutans adhesion at 10 weeks after the immunization. The colonies and depth of the biofilm in the saliva-pretreated group were 41.79% and 41.02%, respectively, less than the control group. The colonies and depth of the biofilm in the co-culture group were 27.4% and 22.81% less than the control group. The assembly of S. mutans and S-IgA was observed under CLSM after co-cultivation. In the mature-stage biofilm, no differences were observed between the different groups.Conclusion: These results demonstrate that the anti-caries DNA vaccine induces the production of specific S-IgA antibodies that may prevent dental caries by inhibiting the initial adherence of S. mutans onto tooth surfaces, thereby reducing the accumulation of S. mutans on the acquired pellicles.

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“…It was observed that this effect is mediated not only by micellar casein, which externally exposes peptides of the K-casein fraction, namely, caseinoglycomacropeptide (CGMP), but also by peptides of the f-casein fraction, such as CPP, which are not exposed externally in the undigested casein molecule (Holt, 1992). In vitro studies had previously demonstrated a restriction of adherence of mutans streptococci to saliva-coated hydroxyapatite beads by milk casein derivatives (Neeser ct al., 1994;Vacca-Smith et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Incorporation of Caseinoglycomacropeptide and Caseinophosphopeptide into the Salivary Pellicle Inhibits Adherence of Mutans Streptococci

et al. 1996

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Incorporation of caseinoglycomacropeptide and caseinophosphopeptide into the salivary pellicle inhibits adherence of mutans streptococci Schüpbach, P; Neeser, J R; Golliard, M; Rouvet, M; Guggenheim, B Schüpbach, P; Neeser, J R; Golliard, M; Rouvet, M; Guggenheim, B. Incorporation of caseinoglycomacropeptide and caseinophosphopeptide into the salivary pellicle inhibits adherence of mutans streptococci. J. Dent. Res. 1996, 75(10) Incorporation of caseinoglycomacropeptide and caseinophosphopeptide into the salivary pellicle inhibits adherence of mutans streptococci AbstractThe protective effects of milk and milk products against dental caries have been demonstrated in many animal studies. We have shown that this effect was mediated by micellar casein or caseinopeptide derivatives. A reduction in the Streptococcus sobrinus population in the oral microbiota of animals fed diets supplemented with these milk components was consistently observed. A possible explanation for these findings is that milk components are incorporated into the salivary pellicle, thereby reducing the adherence of S. sobrinus. This hypothesis was tested in vitro by the incubation of bovine enamel discs with unstimulated saliva. The resulting pellicle was washed and incubated with caseinoglycomacropeptide (CGMP) and/or caseinophosphopeptide (CPP) labeled with 17-and 12-nm gold particles. All samples were prepared for electron microscopy by high-pressure freezing followed by freeze-substitution. It was demonstrated by high-resolution scanning electron microscopy with back-scattered electron imaging, as well as by transmission electron microscopy, that both peptides were incorporated into the pellicle in exchange for albumin, confirming previous findings. This protein was identified with a mouse anti-human serum albumin followed by goat anti-mouse IgG labeled with 25-nm gold particles. Incorporation of CGMP and/or CPP into salivary pellicles reduced the adherence of both S. sobrinus and S. mutans significantly. It is suggested that the calcium and phosphate-rich micellar casein or caseinopeptides are incorporated into the pellicle. The resulting ecological shifts, together with the increased remineralization potential of this biofilm, may explain its modified cariogenic potential.

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The effect of milk and casein proteins on the adherence of Streptococcus mutans to saliva-coated hydroxyapatite

Cited by 92 publications

References 35 publications

Inhibition of adhesion of Streptococcus mutans to hydroxylapatite by commercial dairy powders and individual milk proteins

Inhibition of adhesion of Streptococcus mutans to hydroxylapatite by commercial dairy powders and individual milk proteins

Anti-caries DNA vaccine-induced secretory immunoglobulin A antibodies inhibit formation of Streptococcus mutans biofilms in vitro

Incorporation of Caseinoglycomacropeptide and Caseinophosphopeptide into the Salivary Pellicle Inhibits Adherence of Mutans Streptococci

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