The effect of mixtures of Bacillus thuringiensis-based insecticide and multiple nucleopolyhedrovirus of Lymantria dispar L. in combination with an optical brightener on L. dispar larvae

Akhanaev, Yuriy; Pavlushin, Sergey V.; Polenogova, Olga; Klementeva, Tatyana N.; Lebedeva, D. I.; Okhlopkova, Olesya V.; Kolosov, Alexey; Martemyanov, V. V.

doi:10.1007/s10526-022-10137-7

Cited by 11 publications

(8 citation statements)

References 59 publications

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“…This effect was pronounced at the 1st and 5th instar stages in larvae exposed to NPV in combination with low‐concentration Btk. Our results were consistent with those of the previous study that reported antagonistic effects of the combined application of Btk and LdMNPV in L. dispar (Akhanaev et al, 2022). Similarly, antagonistic effects of Btk and NPV combinations were reported in Helicoverpa armigera and Spodoptera littoralis larvae (Hatem et al, 2012; Liu et al, 2006).…”

Section: Discussionsupporting

confidence: 94%

Effects of Lymantria dispar multiple nucleopolyhedrovirus and Bacillus thuringiensis var. kurstaki on different larval instars of Lymantria dispar asiatica

Hwang

Acharya

Lucas

et al. 2023

Arch Insect Biochem Physiol

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Outbreaks of Lymantria dispar asiatica (the Asian spongy moth; Lepidoptera: Erebidae) occur sporadically, causing widespread damage to forest and fruit trees. Owing to the development of pesticide resistance and environmental contamination, biopesticides, including L. dispar multiple nucleopolyhedrovirus (LdMNPV) and Bacillus thuringiensis var. kurstaki (Btk), can significantly contribute to controlling overall larval stage of this species. Although both pathogens are highly effective at the larval stage, their effects on different instar stages have not been investigated. In this study, we analyzed the mortality and lethality in different L. dispar asiatica instars exposed to single or combined pathogen treatments. Treatments with low or medium LdMNPV concentrations induced lower mortality and had higher LT50 values at the 4th and 5th instars compared with other instars, whereas high LdMNPV treatments induced high mortality in all instars, with higher LT50 values at later instars. Treatment with Btk induced a rapid 100% mortality in all instars, with higher LT50 values for the later instars. The combination of LdMNPV and Btk delayed the killing time compared with the effects of single treatments, with the effect being more pronounced in the 1st and 5th instar stage than at other stages at low Btk concentrations. Our findings indicate that the pathogenic effects of LdMNPV and Btk on L. dispar asiatica differ according to larval stage, thereby providing novel insights into enhancing the biological control efficacy of these agents against L. dispar asiatica in the field.

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Section: Discussionsupporting

confidence: 94%

Effects of Lymantria dispar multiple nucleopolyhedrovirus and Bacillus thuringiensis var. kurstaki on different larval instars of Lymantria dispar asiatica

Hwang

Acharya

Lucas

et al. 2023

Arch Insect Biochem Physiol

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“…Most larvae were dead 7 days after treatment when B. thuringiensis was used in combination with Spodoptera litura NPV showed synergetic activity (Yalashetti et al 2020). As a complement to this observation, when large concentrations of B. thuringiensis kurstaki commercial product Lepidocide were tested with the baculovirus LdMNPV against Lymantria dispar Linnaeus, 1758, larvae, antagonistic effect was observed between both pathogens (Akhanaev et al 2022). This might suggest that sublethal concentrations of B. thuringiensis might increase susceptibility of larvae to virus, while lethal concentrations of the bacterium obstruct, somehow, pathogenic effect of the virus.…”

Section: Discussionmentioning

confidence: 95%

Antagonism between a Baculovirus and Bacillus thuringiensis against Plutella xyllostella 1 Larvae at Laboratory and Field Conditions

Carrasco-Baeza,

Tamayo-Mejía,

Ibarra

et al. 2023

Southwestern Entomologist

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“…1 One solution for reducing inconsistency and increasing competitiveness could be to combine microbial control agents, exploiting their potential complementarity. [6][7][8] Diversity studies with other natural enemies have shown additive or synergistic interactions, through either resource partitioning 9,10 or interspecific facilitation, where the presence of one natural enemy species increases prey susceptibility to another species. 6,11 However, when combining biological control agents, one needs to be aware of potential intraguild interactions which lead to competition, interference or exclusion of one or more natural enemies and can severely limit the benefits of increasing diversity.…”

Section: Introductionmentioning

confidence: 99%

“…However, their relatively slow speed of kill, sometimes limited host range and susceptibility to environmental conditions have impacted their efficacy and reliability in the field, which can be costly for growers 1 . One solution for reducing inconsistency and increasing competitiveness could be to combine microbial control agents, exploiting their potential complementarity 6–8 . Diversity studies with other natural enemies have shown additive or synergistic interactions, through either resource partitioning 9,10 or interspecific facilitation, where the presence of one natural enemy species increases prey susceptibility to another species 6,11 .…”

Section: Introductionmentioning

confidence: 99%

Compatibility of the fungus Beauveria bassiana and Trichoplusia ni SNPV against the cabbage looper Trichoplusia ni: crop plant matters

Deschodt,

Cory

2024

Pest Management Science

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BACKGROUNDMicrobial insecticides are an important weapon in insect pest management, but their use is still relatively limited. One approach for increasing their efficacy and use could be to combine different pathogens to increase pest mortality. However, little is known about whether increasing pathogen diversity will improve pest management. Here, we investigated the compatibility of two pathogens for the management of the cabbage looper, Trichoplusia ni, T. ni nucleopolyhedrovirus (TniSNPV) and the entomopathogenic fungus Beauveria bassiana, on two crops, tomato and broccoli. The pathogens were applied to individual plants using ultra low volume sprays, alone or in combination, either synchronously or asynchronously. Healthy third‐instar T. ni larvae were introduced to the plants before application and collected by destructive sampling 24 h after the last pathogen application.RESULTSCombined applications did not result in an increase in larval mortality compared to TniSNPV alone, although mortality was generally high. B. bassiana was considerably less effective on broccoli compared to tomato. In both the combined treatments, virus‐induced mortality was approximately 50% lower when applied together with the fungus, while fungus‐induced mortality was not affected by the virus, even when the virus was introduced 24 h before the fungus.CONCLUSIONWhile our results suggest that applying this combination of entomopathogens would not be beneficial for pest management, this study illustrates the need to consider the target crop as an important driver of the efficacy of both single and mixed pathogen applications in the field. © 2024 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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The effect of mixtures of Bacillus thuringiensis-based insecticide and multiple nucleopolyhedrovirus of Lymantria dispar L. in combination with an optical brightener on L. dispar larvae

Cited by 11 publications

References 59 publications

Effects of Lymantria dispar multiple nucleopolyhedrovirus and Bacillus thuringiensis var. kurstaki on different larval instars of Lymantria dispar asiatica

Effects of Lymantria dispar multiple nucleopolyhedrovirus and Bacillus thuringiensis var. kurstaki on different larval instars of Lymantria dispar asiatica

Antagonism between a Baculovirus and Bacillus thuringiensis against Plutella xyllostella 1 Larvae at Laboratory and Field Conditions

Compatibility of the fungus Beauveria bassiana and Trichoplusia ni SNPV against the cabbage looper Trichoplusia ni: crop plant matters

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