The effect of moderate exercise on the elevation of Bax/Bcl-2 ratio in oral squamous epithelial cells induced by benzopyrene

Irmawati, Anis; Jasmin, Nadira; Sidarningsih,

doi:10.14202/vetworld.2018.177-180

Cited by 7 publications

(10 citation statements)

References 24 publications

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“…In theory, Bcl‐2 prevents apoptosis, while Bax plays a role in promoting apoptosis . In addition, the ratio of Bax/Bcl‐2 is believed to be a reliable indicator of apoptosis . Interestingly, a previous study showed that the knockdown of NLRP3 protects cells from apoptosis .…”

Section: Discussionmentioning

confidence: 99%

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Ginsenoside Rg1 attenuates cardiomyocyte apoptosis and inflammation via the TLR4/NF‐kB/NLRP3 pathway

Luo

Yan

Sun

et al. 2019

J of Cellular Biochemistry

152

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Sepsis‐induced myocardial dysfunction (SIMD) causes high mortality in seriously ill patients. Ginsenoside Rg1 has been proven to have effective anti‐inflammatory and antiapoptotic properties. However, the specific role of Rg1 in SIMD and the molecular mechanism remain unclear. Hence, we aimed to investigate the latent effects of ginsenoside Rg1 against SIMD and explore its underlying mechanisms. Male C57BL/6J mice and neonatal rat cardiomyocytes (NRCMs) were used as in vivo and in vitro models, respectively. Western blot analysis was used to detect the level of protein expression, and reverse transcription polymerase chain reaction was conducted to determine the messenger RNA expression of inflammatory factors. The terminal deoxynucleotidyl transferase‐mediated nick end labeling assay and flow cytometry were used to determine the apoptosis rate. Echocardiography was performed to assess cardiac function. The results showed that Rg1 improved cardiac function and attenuated lipopolysaccharide (LPS)‐induced apoptosis and inflammation in mice. In addition, in NRCMs, Rg1 downregulated the expression of LPS‐induced inflammatory cytokines and reversed the increased expression of Toll‐like receptor 4 (TLR4), nuclear factor‐κB (NF‐κB), and NOD‐like receptor 3 (NLRP3). In addition, treatment with TLR4 small interfering RNA (siRNA), a p‐NF‐κB inhibitor, or NLRP3 siRNA suppressed LPS‐induced apoptosis and inflammation. In conclusion, Rg1 can attenuate LPS‐induced inflammation and apoptosis both in NRCMs and septic mice and restore impaired cardiac function. Moreover, Rg1 may exert its effect via blocking the TLR4/NF‐κB/NLRP3 pathway.

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Section: Discussionmentioning

confidence: 99%

“…37 In addition, the ratio of Bax/Bcl-2 is believed to be a reliable indicator of apoptosis. 38 Interestingly, a previous study showed that the knockdown of NLRP3 protects cells from apoptosis. 10 This finding suggests that Rg1 can inhibit LPS-induced apoptosis via the TLR4/NF-κB/NLRP3 axis.…”

mentioning

confidence: 99%

Ginsenoside Rg1 attenuates cardiomyocyte apoptosis and inflammation via the TLR4/NF‐kB/NLRP3 pathway

Luo

Yan

Sun

et al. 2019

J of Cellular Biochemistry

152

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“…In Mus musculus test subjects, moderate-intensity exercise (swimming) significantly increased the ratio of Bax/Bcl-2 (n = 6, p = 0.00) and caspase-3 expression (n = 18, p = 0.00) in oral squamous epithelial cells that had been injected with benzopyrene, a carcinogenic substance commonly found in cigarettes. 43,44 The Bax/Bcl-2 ratio, as well as caspase-3, are components in cells that, when their expression increases, receive a signal to perform apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Exercise as a method to reduce the risk of oral cancer: A narrative review

et al. 2022

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Background: Cancer is a major cause of death worldwide. One of the most common forms of cancer is oral cancer, which can occur due to exposure to carcinogenic factors, such as tobacco cigarettes, alcohol, betel-nut chewing, ultraviolet rays or human papillomavirus infection. Physical exercise is known to have many benefits and can contribute to reducing the risk of cancer, minimising the side-effects of treatment and increasing the curative effect of cancer treatment. Purpose: This study aimed to explain the role of exercise as a method to reduce oral cancer risk. Reviews: Studies examining the impact of exercise on reducing oral cancer risk are currently limited due to a lack of research on this subject. However, according to several laboratory experimental research studies on Mus musculus test subjects, moderate-intensity exercise contributes to suppressing the proliferation and development of oral squamous epithelial cells, which can subsequently become cancer cells. Exercise can also increase intracellular proteins that can induce apoptosis in cells (e.g. wild protein p53, the ratio of Bax/Bcl-2, and caspase-3), and can also decrease p53 mutant expression and transformed cells that can trigger cancer. Exercise must be optimally performed to prevent or control cancer symptoms, although the exact duration and intensity of exercise required to reduce cancer risk in humans have not been established. Conclusion: Exercise plays a role in reducing oral cancer risk by inducing apoptosis and preventing the development of transformed cells that can lead to developing cancer.

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“…B[α]P-contaminated foods include vegetables (13 ng/kg), cereals (262 ng/kg), smoked fish (800-13900 ng/kg), and dairy products (11-78 ng/kg) ( 4 ). After entering into the host’s body, B[α]P would bind to the aryl-hydrocarbon receptor and be activated by cytochrome P-4501A1 (CYP1A1) to produce immense size DNA adducts and ROS ( 2 , 5 ), which would inactivate tumor suppressor genes or activate oncogenes, resulting in DNA damage, mutation, and cancer ( 6 , 7 ).…”

Section: Introductionmentioning

confidence: 99%

The Protection of Lactiplantibacillus plantarum CCFM8661 Against Benzopyrene-Induced Toxicity via Regulation of the Gut Microbiota

Zhang

Duan

et al. 2021

Front. Immunol.

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The present study evaluated the protection of Lactiplantibacillus plantarum CCFM8661, a candidate probiotic with excellent benzopyrene (B[a]P)-binding capacity in vitro, against B[a]P-induced toxicity in the colon and brain of mice. Mice that received B[a]P alone served as the model group. Each mouse in the L. plantarum treatment groups were administered 2×109 colony forming unit (CFU) of L. plantarum strains once daily, followed by an oral dose of B[a]P at 50 mg/kg body weight. Behavior, biochemical indicators in the colon and brain tissue, and the gut microbiota composition and short-chain fatty acid (SCFA) levels in the gut were investigated. Compared to the treatment in the model group, CCFM8661 treatment effectively reduced oxidative stress in the brain, improved behavioral performance, increased intestinal barrier integrity, and alleviated histopathological changes in mice. Moreover, CCFM8661 increased the gut microbiota diversity and abundance of Ruminococcus and Lachnospiraceae and reduced the abundance of pro-inflammatory Turicibacter spp. Additionally, the production of SCFAs was significantly increased by L. plantarum CCFM8661. Our results suggest that CCFM8661 is effective against acute B[a]P-induced toxicity in mice and that it can be considered as an effective and easy dietary intervention against B[a]P toxicity.

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The effect of moderate exercise on the elevation of Bax/Bcl-2 ratio in oral squamous epithelial cells induced by benzopyrene

Cited by 7 publications

References 24 publications

Ginsenoside Rg1 attenuates cardiomyocyte apoptosis and inflammation via the TLR4/NF‐kB/NLRP3 pathway

Ginsenoside Rg1 attenuates cardiomyocyte apoptosis and inflammation via the TLR4/NF‐kB/NLRP3 pathway

Exercise as a method to reduce the risk of oral cancer: A narrative review

The Protection of Lactiplantibacillus plantarum CCFM8661 Against Benzopyrene-Induced Toxicity via Regulation of the Gut Microbiota

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