The effect of moderate to severe endometriosis on expression of growth differentiation factor‐9 mRNA in human granulosa cells under controlled ovarian hyperstimulation

Kawabe, Sachiko; Yamashita, Yoshiki; Saito, Natsuho; Kokunai, Kana; Hayashi, Akira; Hayashi, Masami; Miyazaki, Kazunori; Ohmichi, Masahide

doi:10.1007/s12522-015-0208-6

Cited by 7 publications

(5 citation statements)

References 30 publications

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“…This may be expected as these are oocyte paracrine factors that are exclusively secreted by oocytes and therefore appear in the circulation in very low concentrations and thus cannot always be readily detected using existing ELISAs. These findings are consistent with recent studies [15,21] and may explain differences with other studies which reported an association between these markers and endometriosis in follicular fluid using semi-quantitative western blotting, such as Kawabe et al reporting significant reduction in GDF9 mRNA expression in granulosa cells of women with moderate to severe endometriosis [19,20]. It is also possible that the assays are not detecting all forms of the circulating proteins, although the assays target the mature regions of GDF9 and BMP15, and the mature dimers are the forms in which these proteins bind to their signalling receptors [32].…”

Section: Discussionsupporting

confidence: 91%

“…A previous study reported a lower concentration of GDF9 in the follicular fluid of patients with severe endometriosis [19]. Kawabe and colleagues [20] also showed lower GDF9 mRNA expression in granulosa cells of patients with endometriosis compared to controls. However, they did not find differences in GDF9 concentration in follicular fluid from patients with endometriosis.…”

Section: Discussionmentioning

confidence: 89%

“…Mutations that reduce or disrupt the production of these oocyte-secreted factors have been observed in conditions associated with subfertility [14] including polycystic ovary syndrome (PCOS), primary ovarian insufficiency (POI) and Turners syndrome [16][17][18]. An indication that aberrant GDF9 function may be associated with endometriosis arises from reports of lower concentrations of GDF9 protein and mRNA in follicular fluid of patients with severe endometriosis [19,20]. However, until recently [15], there have not been assay methods available to reliably measure GDF9 or BMP15 in human sera and thus assess if concentrations are altered in patients with endometriosis.…”

Section: Introductionmentioning

confidence: 99%

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Oocyte-Secreted Serum Biomarkers GDF9 and BMP15 in Women with Endometriosis

et al. 2022

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Oocyte-secreted growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) are critical paracrine regulators of female fertility. Recent studies demonstrated that serum concentrations are associated with the number of oocytes retrieved during IVF, and therefore potential clinical use as biomarkers. However, it is unknown if the presence of endometriosis affects serum GDF9 or BMP15. An exploratory case–control study was prospectively performed on 60 women who underwent laparoscopy between April 2017 and August 2018 at two hospitals. GDF9 and BMP15 were measured by validated immunoassays in pre-operative serum samples. Data were analysed relative to laparoscopic assessment of endometriosis and staging. There were 35 women with confirmed laparoscopic diagnosis of endometriosis and 25 controls with no evidence of endometriosis at laparoscopy. GDF9 was detectable in 40% of controls and 48% of cases. There was no difference in median GDF9 concentrations between controls (20.0 pg/ml, range 20.0–2504 pg/ml) and cases (20.0 pg/ml, range 20.0–2963 pg/ml). BMP15 was detectable in 48% of controls and 58% of cases, with no difference in median concentrations between controls (26.5 pg/ml, range 24.0–1499 pg/ml) and cases (24.0 pg/ml, range 24.0–796 pg/ml). Furthermore, there were no significant differences in the proportion of detectable samples or concentrations of GDF9 or BMP15 with differing severities of endometriosis. In conclusion, serum concentrations of oocyte-secreted factors, GDF9 and BMP15 did not differ between control patients and patients with endometriosis. For clinical application in reproductive medicine, GDF9 and BMP15 serum biomarker quantitation is unlikely to be aberrant in the presence of endometriosis.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

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Oocyte-Secreted Serum Biomarkers GDF9 and BMP15 in Women with Endometriosis

et al. 2022

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“…Follicular fluids (FFs) surround the granulosa cell-oocyte complex and mediate factors in the communication between the cells within the follicle [ 19 ]. Recently, different studies have investigated the changes in the FF composition of women with endometriosis, such as gonadal hormones [ 20 ], growth factors [ 21 ], and cytokines [ 22 ], and accumulating evidence suggests that the hormone microenvironment contributes to the oocyte maturation, oocyte quality, and even the subsequent embryonic development. However, there is conflicting data in the literatures concerning the level of the key FF hormones.…”

Section: Discussionmentioning

confidence: 99%

The Mechanism Exploration of Follicular Fluids on Granulose Cell Apoptosis in Endometriosis-Associated Infertility

Chen

Zhang³

et al. 2021

BioMed Research International

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Objective. To explore the mechanisms of follicular fluids (FFs) on granulose cell (GC) apoptosis in endometriosis-associated infertility. Materials and Methods. 60 infertile women were enrolled. The FFs from 30 endometriosis-associated infertility (EI) patients were collected and processed by ELISA hormone assay and proteomic profiling. The ovary GCs collected from 30 tubal-associated infertility (TI) patients were cultured in follicular fluids of endometriosis-associated infertility patients (EI-FFs), and the apoptosis mechanisms were explored by flow cytometry assay, real-time PCR, Western blotting, and protein–protein interaction (PPI) network analysis. Results. Our results showed that the expression of 22 specific proteins was significantly different in the FFs from EI and TI patients, and the level of testosterone and anti-Müllerian hormone was not obviously different between the two groups. EI-FFs could accelerate the apoptosis process of granulose cells of tubal-associated infertility patients (TI-GCs) by regulating the expression of 5 apoptosis-related proteins including BCL2, BAX, CASP3, CASP9, and TP53. The correlation of these 22 specific proteins and 5 apoptosis-related proteins was analyzed by PPI, and 5 protein biomarkers (INS, CXCL10, ICAM1, WIF1, and TNFRSF13C) and 5 signaling pathways (cytokine-cytokine receptor interaction, apoptosis, regulation of actin cytoskeleton, MAPK, and p53 signaling pathway) were predicted. Conclusion. This research clarified the effect and explored the mechanisms of EI-FFs on the apoptosis of TI-GCs and indicated the protein biomarkers and signaling pathways for further study.

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“…In the following error was published on page 181 under the heading “Patients’ characteristics” of “Results”.…”

mentioning

confidence: 99%