The effect of modified polysialic acid based hydrogels on the adhesion and viability of primary neurons and glial cells

Haile, Yohannes; Berski, Silke; Dräger, Gerald; Nobre, André; Stummeyer, Katharina; Gerardy‐Schahn, Rita; Grothe, Claudia

doi:10.1016/j.biomaterials.2007.12.030

Cited by 45 publications

(22 citation statements)

References 39 publications

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“…14,15 Further, we already demonstrated that soluble polySia as well as first chemically modified derivates provided favorable substrates and that polySia degradation products were not toxic for glial and neuronal cells in vitro. 16,17 Here we present in vivo data on the complementation of synthetic nerve transplants with exogenous soluble polySia, isolated from Escherichia coli K1 (K1-polySia) capsules (degree of polymerization >60 18 ). Regeneration across sciatic nerve gaps in rat was studied after reconnecting the transected nerve stumps with differentially filled silicon tubes (absence or presence of SC and K1-polySia).…”

Section: Introductionmentioning

confidence: 99%

In Vivo Evaluation of Polysialic Acid as Part of Tissue-Engineered Nerve Transplants

Haastert‐Talini

Schaper-Rinkel²,

Schmitte³

et al. 2010

Tissue Engineering Part A

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With the aim to develop new biomaterials for peripheral nerve grafts, the current study used bioidentical polysialic acid (polySia) as complement in synthetic conduits. polySia provides an important guidance cue during nervous system development and regeneration. First in vivo results on the use of cell-free and Schwann cell-containing synthetic peripheral nerve grafts complemented with soluble exogenous K1-polySia are presented. Reconstructing 10 mm rat sciatic nerve gaps, K1-polySia complementation significantly improved structural nerve regeneration in comparison to cell-free and K1-polySia-free grafts. Subsequently, long nerve gaps (13 mm) were reconstructed by Schwann cell transplants plus K1-polySia and compared to nerve autotransplantation. Structural but also functional regeneration could be observed using K1-polySia transplants; however, autotransplantation was still significantly more successful. Overall, the current study demonstrates that exogenous K1-polySia has no negative but rather regeneration promoting effects. This is important novel evidence on the applicability of exogenous polySia in vivo. Further studies are required to develop solid three-dimensional polySia-based scaffolds for nerve tissue engineering. Biocompatible and assessable biodegrading materials will ensure long-lasting presence of polySia to allow its applicability and prolonged efficacy in the slow regenerating scenario of human peripheral nerve reconstruction.

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Section: Introductionmentioning

confidence: 99%

In Vivo Evaluation of Polysialic Acid as Part of Tissue-Engineered Nerve Transplants

Haastert‐Talini

Schaper-Rinkel²,

Schmitte³

et al. 2010

Tissue Engineering Part A

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“…For example, cellulose (e.g., CA used in this study) is an excellent structural material (indeed, is one of the most abundant structural biomacromolecules used throughout nature and industry) but lacks amino groups found in most mammalian glycosoaminoglycans (GAGs) and other polysaccharides such as polysialic acid (PSA). PSA in particular is an intriguing amino-bearing polysaccharide shown to have benefits for neural guidance in tissue engineering applications (Haile et al, 2006), but PSA fibers have poor stability in aqueous milieu limiting their use to hydrogel formats (Haile et al, 2008) or immobilization on glass (Steinhaus et al, 2010). Similarly other GAGs, without substantial chemical modification such as the introduction of stabilizing crosslinkers, are not suitable for nanofiber construction.…”

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of chitosan, cellulose acetate, and polyethersulfone nanofiber scaffolds for neural differentiation

Tan

Kim

et al. 2014

Carbohydrate Polymers

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Based on accumulating evidence that the 3D topography and the chemical features of a growth surface influence neuronal differentiation, we combined these two features by evaluating the cytotoxicity, proliferation, and differentiation of the rat PC12 line and human neural stem cells (hNSCs) on chitosan (CS), cellulose acetate (CA), and polyethersulfone (PES)-derived electrospun nanofibers that had similar diameters, centered in the 200 to 500 nm range. None of the nanofibrous materials were cytotoxic compared to 2D (e.g., flat surface) controls; however, proliferation generally was inhibited on the nanofibrous scaffolds although to a lesser extent on the polysaccharide-derived materials compared to PES. In an exception to the trend towards slower growth on the 3D substrates, hNSCs differentiated on the CS nanofibers proliferated faster than the 2D controls and both cell types showed enhanced indication of neuronal differentiation on the CS scaffolds. Together, these results demonstrate beneficial attributes of CS for neural tissue engineering when this polysaccharide is used in the context of the defined 3D topography found in electrospun nanofibers.

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“…EndoNF has been established as a unique tool to degrade specifically artificial polysialic acid hydrogels and other polysialic acid derivatives in a biocompatible and induced manner [179][180][181][182]. In a mouse femoral nerve regeneration model it has been found that if endosialidase is injected into the lesion site polysialic acid reexpression in the regenerating axons is abolished.…”

Section: Applications Of Active Endosialidase For the Study Of Ncammentioning

confidence: 99%

“…At the same time, the regeneration accuracy is hampered, which indicates a role of polysialic acid in nerve regeneration [183]. In future practical applications of polysialic acid as scaffold of neural regeneration, endosialidase may become useful as an agent of specific degradation of the scaffold to avoid second surgery [180].…”

Section: Applications Of Active Endosialidase For the Study Of Ncammentioning

confidence: 99%

Endosialidases: Versatile Tools for the Study of Polysialic Acid

Jakobsson

Schwarzer

Jokilammi

et al. 2012

Topics in Current Chemistry

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Polysialic acid is an α2,8-linked N-acetylneuraminic acid polymer found on the surface of both bacterial and eukaryotic cells. Endosialidases are bacteriophage-borne glycosyl hydrolases that specifically cleave polysialic acid. The crystal structure of an endosialidase reveals a trimeric mushroom-shaped molecule which, in addition to the active site, harbors two additional polysialic acid binding sites. Folding of the protein crucially depends on an intramolecular C-terminal chaperone domain that is proteolytically released in an intramolecular reaction. Based on structural data and previous considerations, an updated catalytic mechanism is discussed. Endosialidases degrade polysialic acid in a processive mode of action, and a model for its mechanism is suggested. The review summarizes the structural and biochemical elucidations of the last decade and the importance of endosialidases in biochemical and medical applications. Active endosialidases are important tools in studies on the biological roles of polysialic acid, such as the pathogenesis of septicemia and meningitis by polysialic acid-encapsulated bacteria, or its role as a modulator of the adhesion and interactions of neural and other cells. Endosialidase mutants that have lost their polysialic acid cleaving activity while retaining their polysialic acid binding capability have been fused to green fluorescent protein to provide an efficient tool for the specific detection of polysialic acid.

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The effect of modified polysialic acid based hydrogels on the adhesion and viability of primary neurons and glial cells

Cited by 45 publications

References 39 publications

In Vivo Evaluation of Polysialic Acid as Part of Tissue-Engineered Nerve Transplants

In Vivo Evaluation of Polysialic Acid as Part of Tissue-Engineered Nerve Transplants

Comparative evaluation of chitosan, cellulose acetate, and polyethersulfone nanofiber scaffolds for neural differentiation

Endosialidases: Versatile Tools for the Study of Polysialic Acid

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