The effect of modifying carbohydrate metabolism on seed protein gene expression in peas

Casey, R.; Domoney, Claire; Forster, C.; Hedley, C. L.; Hitchin, Ed; Wang, Trevor

doi:10.1016/s0176-1617(98)80023-0

Cited by 23 publications

(28 citation statements)

References 30 publications

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“…; Casey et al . ). The importance of this lack of SAAs relates to whether or not pea is consumed as part of a balanced diet, as cereal crops provide SAAs lacking in pulses if consumed concurrently and pulses provide lysine, an amino acid which is lacking in cereals (Shewry & Tatham ).…”

Section: Proteinmentioning

confidence: 97%

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Improving pulse crops as a source of protein, starch and micronutrients

2019

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Pulse crops have been known for a long time to have beneficial nutritional profiles for human diets but have been neglected in terms of cultivation, consumption and scientific research in many parts of the world. Broad dietary shifts will be required if anthropogenic climate change is to be mitigated in the future, and pulse crops should be an important component of this change by providing an environmentally sustainable source of protein, resistant starch and micronutrients. Further enhancement of the nutritional composition of pulse crops could benefit human health, helping to alleviate micronutrient deficiencies and reduce risk of chronic diseases such as type 2 diabetes. This paper reviews current knowledge regarding the nutritional content of pea (Pisum sativum L.) and faba bean (Vicia faba L.), two major UK pulse crops, and discusses the potential for their genetic improvement.

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Section: Proteinmentioning

confidence: 97%

“…Mutations at genetic loci in pea, resulting in decreased starch content (Casey et al . ), also alter legumin: vicilin ratios, total protein concentrations and the relative proportion of the albumin fraction of seed protein (Hughes et al . ).…”

Section: Biofortification Of Pea and Faba Beanmentioning

confidence: 99%

Improving pulse crops as a source of protein, starch and micronutrients

2019

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“…On the other hand, in wheat endosperm the rate and duration of protein deposition seem to be independent of starch biosynthesis (Jenner et al 1991), suggesting that the pathways of starch and protein biosynthesis are not directly connected (Barneix et al 1992). However, in mutant seeds with deletions in the starch-formation pathway, storage-protein accumulation is also affected (Casey et al 1998). Wrinkled pea seed mutants have reduced starch and increased protein contents (Perez et al 1993;Boutin et al 1998), indicating altered partitioning between starch and protein.…”

Section: Introductionmentioning

confidence: 99%

Antisense-inhibition of ADP-glucose pyrophosphorylase in Vicia narbonensis seeds increases soluble sugars and leads to higher water and nitrogen uptake

Rolletschek

Hajirezaei

Wobus

et al. 2002

Planta

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We previously reported on Vicia narbonensis seeds with largely decreased alpha- D-glucose-1-phosphate adenyltransferase (AGP; EC 2.7.7.27) due to antisense inhibition [H. Weber et al. (2000) Plant J 24:33-43]. In an extended biochemical analysis we show here that in transgenic seeds both AGP activity and ADP-glucose levels were strongly decreased but starch was only moderately reduced and contained less amylose. The flux control coefficient of AGP to starch accumulation was as low as 0.08, i.e. AGP exerts low control on starch biosynthesis in Vicia seeds. Mature cotyledons of antisense seeds had increased contents of lipids, nitrogen and sulfur. The protein content was higher due, in particular, to increased sulfur-rich albumins. Globulin fractions of storage proteins had a lower ratio of legumin to vicilin. Isolated cotyledons partitioned less [14C]sucrose into starch and more into soluble sugars with no change in the protein fraction. Respiration of isolated cotyledons and activities of the major glycolytic and carbohydrate-metabolizing enzymes were not affected. Sucrose and the hexose-phosphate pool were increased but UDP-glucose, 3-phosphoglyceric acid, phospho enolpyruvate, pyruvate, ATP and ADP were unchanged or even lower, indicating that carbon partitioning changed from starch to sucrose without affecting the glycolytic and respiratory pathways. Soluble compounds were increased but osmolality remained unchanged, indicating compensatory water influx resulting in higher water contents. Developmental patterns of water and nitrogen accumulation suggest a coupled uptake of amino acids and water into cotyledons. We conclude that, due to higher water uptake, transgenic cotyledons take up more amino acids, which become available for protein biosynthesis leading to a higher protein content. Obviously, a substantial part of amino acid uptake into Vicia seeds occurs passively and is osmotically controlled and driven by water influx.

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“…Globulin proteins have higher amount of glutamine aspartic acid, arginine and lysine (Dahl et al 2012). Albumins usually have physiological role present in low amount than globulins constituting only up to 15-25% of total seed protein (Casey et al 1998;Machuca 2000). The albumins have higher amount of cystine, methionine and lysine contents as compared to globulin fractions in beans (Marquez and Lajolo 1981).…”

mentioning

confidence: 99%