The Effect of Mofettes on Oxidative Stress/Antioxidant Balance in Experimental Myocardial Ischemia

Abstract

Background/Aim: Natural mofettes are gases resulting from post-volcanic emanations. This study aimed to examine the effect of mofette therapy on plasma oxidative stress and antioxidant parameters in rats after experimental induction of myocardial ischemia, as well as on structural changes in myocardial tissue. Materials and Methods: White Wistar-Bratislava rats were divided into three groups. In groups 2 and 3, myocardial ischemia was induced by isoproterenol. Rats in group 3 were additionally exposed to high levels mofettes. Oxidative stress and antioxidant parameters were determined in plasma. The structural changes of the myocardium were observed in paraffin embedded slices contrasted using Goldner's trichrome staining. Results: A statistically significant change in serum oxidative stress biomarkers, including nitric oxide, malondialdehyde, total oxidant status, as well as in the tested antioxidant molecules and total antioxidant capacity were observed in group 3 compared to group 2. Also, rats of group 3 showed an obvious improvement in inflammatory infiltration and repair of necrotic areas through collagen proliferation (proliferation of fibrous connective tissue) compared to group 2. Conclusion: Mofette had a beneficial effect on the balance between oxidative stress and antioxidant status following experimentally induced myocardial ischemia. Natural mofettes are gases resulting from natural postvolcanic emanations (1) that are found in certain areas of Romania, e.g. in the Harghita volcanic massif, from which carbon dioxide, present in a 95-98% proportion, is used as a therapeutic factor (2, 3). Along with carbon dioxide, mofette gas contains small amounts of other volcanic emission gases: ammonium, sulfur, helium and radon (4, 5), which induce pulverization of CO 2 molecules, increasing the penetration power of CO 2 (1-3). Mofettes also contain positive and negative air ions, 2,000-15,000 ions/cm 3 , and have a radioactivity of 0.3 μCi/l, without cancer risk, and a radon concentration lower than 1% (4). In Romania, there are natural mofettes in the spa resorts Covasna,

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The Rectus Sheath Block (RSB) Analgesia Following Laparotomy Could Affect Malonidialdehyde (MDA) Concentrations in Benign Disease and Cancer

“…Peroxidation of PUFAs on cell membranes alters cell membrane transport mechanism, membrane channel function and membrane receptor interactions (1). In earlier studies MDA biomarker has been used to show the connection between oxidative stress and chronic obstructive pulmonary disease (COPD) (19), lumbar disc degeneration (LDD) (20), myocardial ischemia (21) and diabetes mellitus (DM) (22). A review by Sardaro et al (6) concluded that association between the most frequent inflammatory, potentially malignant diseases is well established.…”

“…Lu et al 2018 (19) studied the effect of oligomeric proanthocyanidin (OPC) (23) on the oxidative stress and lung function in COPD patients. The results showed that OPC supplementation significantly reduced the plasma SOD and MDA biomarker concentrations and the OPC supplementation was effective in increasing the antioxidant (21) studied the effect of mofettes which are gases resulting from natural post-volcanic emnations that are found in certain areas of Romania. They used Wistar-Bratislava rats divided into three groups: 1, control group; 2, isoproterenolinduced myocardial ischemia group; 3, isoproterenol-induced myocardial ischemia+mofette group.…”

Therapeutic gases used in balneotherapy and rehabilitation medicine - scientific relevance in the last ten years (2011 – 2020) - Synthetic literature review

“…During therapy, CO2 inhaled and dissolved in plasma, and absorbed into the skin, has many effects on the body; thus, at the level of peripheral circulation, it induces skin hyperemia by direct action on metarterioles, with an increase in skin temperature by 1.5-4 degrees C and a 44% increase in skeletal muscle blood flow. There are also effects on cardiac hemodynamics by causing arterial vasodilation, a decrease in the pre-ejection period, an increase in the ejection period, and a reduction in blood pressure with consequences on peripheral oxygen use, which explains the improvement of cardiac performance parameters as well as the exercise capacity in coronary patients and patients with myocardial infarction sequelae (12). The mofettes used for therapeutic purposes in Romania, in the Hospital of Rehabilitation in Cardiovascular diseases Covasna, are unique in the world.…”