The Effect of Monooxygenase and Glutathione S-Transferase Inhibitors on the Metabolism of Diclofop-methyl and Fenoxaprop-ethyl in Barley and Wheat

Romano, M.; Stephenson, G. R.; Tal, A.; Hall, J. Christopher

doi:10.1006/pest.1993.1049

Cited by 40 publications

(33 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition to the compounds listed in Table 1, P450s have been implicated in the metabolism of several herbicides by in vivo studies in which the changes in herbicidal activity and/or the rate of herbicide metabolism were measured in response to various P450 inhibitors. Unconfirmed by in vitro data, the involvement of P450s are suggested by in vivo results in the metabolism of chlortoluron in wheat (Canivenc et al 1989;Mougin et al 1992), corn and cotton (Cole and Owen 1987); bentazon in soybean (Sterling and Balke 1990); pyrazosulfuron ethyl in rice (Yun et al 2001); clodinafop in wheat, barley and corn (Kreuz et al 1991); phenoxaprop-p-ethyl in barley (Romano et al 1993); clomazone in cotton (Ferhatoglu et al 2005); thiazopyr in several weedy species (Feng et al 1995) and EPTC in some cereals (Barta and Dutka 1991). (Additional examples are discussed under ''P450-Endowed Herbicide Resistance in Weeds'').…”

Section: Several Herbicides Are Metabolized By P450s In Plantsmentioning

confidence: 78%

Plant cytochrome P450-mediated herbicide metabolism

2006

View full text Add to dashboard Cite

In the last two decades it has become apparent that enzymes of the P450 monooxygenase (P450) superfamily are responsible for the Phase I metabolism of numerous herbicides representing several classes of organic compounds. The majority of experimental evidence for P450 involvement in herbicide metabolism has been derived from in vitro studies in which the catalytic activity of plant microsomes towards herbicidal substrates was measured in the presence of various P450 inhibitors and activators. While the studies with microsomes elicited much appreciation for the pivotal roles of plant P450s in herbicide metabolism, detailed characterization of these enzymes only became possible after the isolation of genes encoding specific isoforms responsible for herbicide conversion. Several lines of evidence suggest that the development of herbicide resistance in weeds by enhanced detoxification is frequently associated with elevated levels of P450 activity. Enhanced detoxification-based herbicide resistance is particularly difficult to control, because it can involve resistance to multiple, chemically unrelated classes of herbicides. Continued research efforts are aimed at elucidating the role of P450s in the metabolic fates of herbicides in plants and the development of herbicide resistance in weeds. Recent advances made in the isolation and genetic manipulation of P450 enzymes have created new opportunities for their application in engineering herbicide tolerance and bioremediation.

show abstract

Section: Several Herbicides Are Metabolized By P450s In Plantsmentioning

confidence: 78%

Plant cytochrome P450-mediated herbicide metabolism

2006

View full text Add to dashboard Cite

show abstract

“…De fato, as fitointoxicações provocadas por herbicidas podem causar inibição do transporte de fotoassimilados das folhas para os outros órgãos da planta (Royuela et al, 2000;Zabalza et al, 2004). A depleção fotossintética provocada pelo mesotrione nos tecidos aéreos das plantas, até o quinto DAA, deve levar a uma redução do conteúdo de açúcares e de outros compostos orgânicos a serem transportados para os tecidos não clorofilados, afetando principalmente as reações biossintéticas nas raízes (Rajcan & Tollenar, 1999).…”

Section: Resultsunclassified

Análise do perfil eletroforético de proteínas citoplasmáticas para verificação do processo de desintoxicação do herbicida mesotrione em plantas de Zea mays

et al. 2014

View full text Add to dashboard Cite

RESUMO -As plantas tolerantes a herbicidas apresentam rotas bioquímicas eficientes na desintoxicação dessas moléculas no interior da célula, e muitas enzimas citoplasmáticas participam desse processo. No presente trabalho, o perfil eletroforético de proteínas citoplasmáticas foi avaliado em folhas, caules e raízes de plantas de milho, durante o processo de desintoxicação, após tratamento com o herbicida mesotrione. Aos 15 dias após o plantio, foram aplicados 192 gramas por hectare (g ha -1) do mesotrione, em pós-emergência; três e sete dias após a aplicação (DAA), foram coletados os tecidos para a realização de fracionamento celular e isolamento das proteínas solúveis do citoplasma. A atividade fotossintética foi analisada como marcador fisiológico do nível de fitointoxicação em diferentes estádios (1, 2, 3, 5 e 7 DAA). Enquanto a fotossíntese foi inibida nos primeiros 3 DAA, não se observou alteração significativa a partir do quinto dia. Medidas biométricas foram realizadas aos 7 DAA, não apresentando diferenças significativas. A análise dos perfis eletroforéticos das proteínas citoplasmáticas indicou maior expressão proteica em regiões de baixa massa molecular (~ de 21 a 65 kDa) nos tecidos de folhas e caules aos 3 DAA do mesotrione. Contudo, aos sete dias observou-se recuperação de perfis semelhantes aos tecidos de plantas não tratadas com o herbicida. Nas raízes, houve redução na biossíntese de proteínas sob tratamento com herbicida, tanto aos 3 quanto aos 7 DAA. Os resultados sugerem que as alterações do perfil eletroforético das proteínas citoplasmáticas das plantas de milho refletem bem o estádio de desintoxicação de seus tecidos e que, mesmo após o processo haver se estabelecido na parte aérea, as raízes continuaram a apresentar alterações, que indicam um processo mais prolongado de desintoxicação do mesotrione sobre o sistema radicular.Palavras-chave: fitotoxicidade, milho, proteína, controle de plantas daninhas, metabolização de herbicida. ABSTRACT -Herbicide-tolerant plants have efficient biochemical pathways for detoxification of these molecules within the cell, with many cytoplasmic enzymes being involved in this process. In this study, the electrophoretic profiles of cytoplasmic proteins were evaluated in the leaves, stems, and roots of corn plants during the detoxification process after treatment with the herbicide mesotrione. At 15 days after planting, 192 grams per hectare (g ha -1) of mesotrione were administered postemergence. Three or seven days after application (DAA), tissues were collected to perform cell fractionation and isolation of soluble proteins from the cytoplasm. Photosynthetic activity was analyzed as a physiological marker of the level of intoxication at different stages (1, 2, 3, 5, and 7 DAA). While photosynthesis was inhibited in the first three days after spraying, there was no significant change on the fifth day. Biometric measurements were performed at seven DAA, and no significant differences were observed. Analysis of electrophoretic profiles of cytoplasmic proteins indi...

show abstract

“…It has been demonstrated in wheat that the metabolism of diclofop, which has the same mechanism of action as fenoxaprop-pethyl, may be mediated by different enzymes, possibly P450-dependent monooxygenases, and the role of GST in diclofop metabolism in wheat has not been demonstrated (Romano et al, 1993). However, the selectivity of the aryloxyphenoxy propionate herbicide class can be increased with the addition of specific safeners.…”

Section: Resultsmentioning

confidence: 99%

Mefenpyr-diethyl action on fenoxaprop-p-ethyl detoxification in wheat varieties

Catâneo

Ferreira

Mischan³

et al. 2013

Planta daninha

View full text Add to dashboard Cite

-Safeners protect crops against herbicide injury. The aim of this study was to examine the differential susceptibility of five wheat (Triticum aestivum) ). Plants were evaluated visually for injury at 7 and 14 days after treatment (DAT). Glutathione S-transferase (GST) activity was assayed in aerial parts at 7 DAT, and lipid content was measured at 14 DAT. Varieties CEP 24, IAPAR 78 and Rubi were more tolerant to fenoxaprop-p-ethyl than BRS 49, and CD 104 rapidly recovering from the slight phytotoxicity symptoms produced by the herbicide. Mefenpyr-diethyl prevented crop injury associated with the herbicide. GST activity did not correlate directly with fenoxaprop-p-ethyl detoxification. However, lipid content was related to the susceptibility of wheat to fenoxaprop-p-ethyl treatment.Keywords: safener, Triticum aestivum, selectivity, glutathione S-transferase, lipid content.RESUMO -Protetores ou safeners protegem culturas contra a injúria de herbicidas. O propósito deste estudo foi investigar a suscetibilidade diferencial de cinco variedades de trigo (Triticum aestivum) ao herbicida fenoxaprop-p-ethyl e o efeito do protetor mefenpyr-diethyl na minimização da injúria do herbicida e sobre o conteúdo de lipídios. As variedades BRS 49, CD 104, CEP 24, IAPAR 78 e Rubi foram pulverizadas com fenoxaprop-p-ethyl (69 g ha -1 ), fenoxaprop-p-ethyl + mefenpyr-diethyl (69 g + 18,75 g ha 1 ) ou mefenpyr-diethyl (18,75 g ha 1 ). A injúria das plantas foi avaliada visualmente aos 7 e 14 dias após o tratamento (DAT). A atividade da glutationa S-transferase (GST) foi analisada na parte aérea aos 7 DAT, e o conteúdo de lipídios, aos 14 DAT. As variedades CEP 24, IAPAR 78 e Rubi foram mais tolerantes ao fenoxaprop-p-ethyl do que a BRS 49, e a variedade CD 104 rapidamente se recuperou dos sintomas de intoxicação produzidos pelo herbicida. Mefenpyrdiethyl preveniu a injúria da cultura pelo herbicida. A atividade da GST não foi correlacionada diretamente com a desintoxicação do fenoxaprop-p-ethyl. Contudo, o conteúdo de lipídios esteve relacionado à suscetibilidade de trigo ao tratamento de fenoxaprop-p-ethyl.Palavras-chave: safener, Triticum aestivum, seletividade, glutationa S-transferase, conteúdo de lipídios.CATANEO, A.C. et al.

show abstract

The Effect of Monooxygenase and Glutathione S-Transferase Inhibitors on the Metabolism of Diclofop-methyl and Fenoxaprop-ethyl in Barley and Wheat

Cited by 40 publications

References 0 publications

Plant cytochrome P450-mediated herbicide metabolism

Plant cytochrome P450-mediated herbicide metabolism

Análise do perfil eletroforético de proteínas citoplasmáticas para verificação do processo de desintoxicação do herbicida mesotrione em plantas de Zea mays

Mefenpyr-diethyl action on fenoxaprop-p-ethyl detoxification in wheat varieties

Contact Info

Product

Resources

About